LOCUS       sequence-495529-        3666 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part Act1 (OsAct) promoter, GGAG - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL12014
FEATURES             Location/Qualifiers
     source          1..3666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        850..1692
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             1695..1712
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2293..2311)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2379..2483
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2705..3496
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 ctagcatact cgaggtcatt catatgcttg agaagagagt cgggatagtc caaaataaaa
      901 caaaggtaag attacctggt caaaagtgaa aacatcagtt aaaaggtggt ataaagtaaa
      961 atatcggtaa taaaaggtgg cccaaagtga aatttactct tttctactat tataaaaatt
     1021 gaggatgttt ttgtcggtac tttgatacgt catttttgta tgaattggtt tttaagttta
     1081 ttcgcttttg gaaatgcata tctgtatttg agtcgggttt taagttcgtt tgcttttgta
     1141 aatacagagg gatttgtata agaaatatct ttaaaaaaac ccatatgcta atttgacata
     1201 atttttgaga aaaatatata ttcaggcgaa ttctcacaat gaacaataat aagattaaaa
     1261 tagctttccc ccgttgcagc gcatgggtat tttttctagt aaaaataaaa gataaactta
     1321 gactcaaaac atttacaaaa acaaccccta aagttcctaa agcccaaagt gctatccacg
     1381 atccatagca agcccagccc aacccaaccc aacccaaccc accccagtcc agccaactgg
     1441 acaatagtct ccacaccccc ccactatcac cgtgagttgt ccgcacgcac cgcacgtctc
     1501 gcagccaaaa aaaaaaaaag aaagaaaaaa aagaaaaaga aaaaacagca ggtgggtccg
     1561 ggtcgtgggg gccggaaacg cgaggaggat cgcgagccag cgacgaggcc ggccctccct
     1621 ccgcttccaa agaaacgccc cccatcgcca ctatatacat acccccccct ctcctcccat
     1681 ccccccaacc ctaccaccac caccaccacc acctccacct cctcccccct cgctgccgga
     1741 cgacgagctc ctcccccctc cccctccgcc gccgccgcgc cggtaaccac cccgcccctc
     1801 tcctctttct ttctccgttt tttttttccg tctcggacac gatctttggc cttggtagtt
     1861 tgggtgggcg agaggcggct tcgtgcgcgc ccagatcggt gcgcgggagg ggcgggatct
     1921 cgcggctggg gctctcgccg gcgtggatcc ggcccggatc tcgcggggaa tggggctctc
     1981 ggatgtagat ctgcgatccg ccgttgttgg gggagatgat ggggggttta aaatttccgc
     2041 catgctaaac aagatcagga agaggggaaa agggcactat ggtttatatt tttatatatt
     2101 tctgctgctt cgtcaggctt agatgtgcta gatctttctt tcttcttttt gtgggtagaa
     2161 tttgaatccc tcagcattgt tcatcggtag tttttctttt catgatttgt gacaaatgca
     2221 gcctcgtgcg gagctttttt gtaggtagaa gccaatgtga gaccacgaag tggctcttca
     2281 gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     2341 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2401 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2461 aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2521 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg
     2581 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2641 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     2701 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     2761 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2821 tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga
     2881 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     2941 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     3001 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3061 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3121 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3181 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3241 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3301 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3361 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3421 ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa
     3481 ggtagtcggc aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3541 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3601 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3661 atcttc
//
