LOCUS       sequence-495532-        3375 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part Lotus Ubiquitin promoter, GGAG - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3375)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL12027
FEATURES             Location/Qualifiers
     source          1..3375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2002..2020)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2088..2192
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2414..3205
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 ggagagagga ttttgaggaa ataattaatt gaattacttg tattattgat aaagtaattt
      901 agataagttg ttagtacaac ttattgcaac tatggataga caaaaatcac ttattttaag
      961 gggagaagta tataacaact tattataaaa tttctgagca gccggcctcc tccaatcaat
     1021 catagagagt ggagcccatt ctggaaaacc aaggaacccc caacttgcac tggtgcggtg
     1081 gcccaattca aaaaaaacgg ggcccagcaa agtaacgccg ttgtaacgat ttatcaatcc
     1141 aaactcaaaa ggcggcgaga tgcgtttaac tccgtgaaat taacaaaccg ccaacaactt
     1201 gcaatttgca actaccgttt ccaagaagaa ctcaaccaca caacgtatcc tatcccaaac
     1261 cacacgcaac tagtgacgcg tcataaggac acgtgtcaca atttgactgg ttaataattt
     1321 caccgctttt gctataaatt acctccaatc cccttagctt cttcacaatt cagttcccaa
     1381 ccctaacaat tcttgttcat atcgcttctc tctactttca aggtatgatc caatttctct
     1441 cttctttctc tgtaatcctt tcgttgagtt ttgtttccga tcaatcatag gtagttttct
     1501 tgtttcgaag catgagatct aggaattttt tgtgattttc caaaattgag atcggtttga
     1561 aattgaattt tacagcttga atctcagatc ttgttttatc aatgttttcg atggcttgcg
     1621 atgtagatct atgataattg tggttcagtt ttgttaggaa tcgatttcgg tttagcaatt
     1681 gcagattaat tagggtttcc aattgaattc ttcagatccg ttatggaatt atgtcaaata
     1741 atttattcaa attggaaatt attgttagat ccactcttaa tctgtttgat ccaagcttca
     1801 attagggttt tcacttgttt caatttcttg ttatggattc tgatttatct gttgatgtta
     1861 gatccactct taatctgttt gatccaagcg ttaattaggg ttttcacttg tttcaatttc
     1921 gtgtgttgga ttctgattta tctgttgttg atgtgattac agaatgtgag accacgaagt
     1981 ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2041 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2101 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2161 aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct
     2221 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2281 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     2341 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     2401 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     2461 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     2521 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
     2581 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     2641 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     2701 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     2761 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     2821 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     2881 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     2941 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     3001 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     3061 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     3121 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     3181 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     3241 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3301 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3361 gatcaaagga tcttc
//
