LOCUS       sequence-495534-        3223 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part pAt5: XET (At5g65730) promoter, GGAG - 
            AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3223)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL12029
FEATURES             Location/Qualifiers
     source          1..3223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1850..1868)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1936..2040
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2262..3053
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 atgtcggcga gtctcactaa gatgttgaag taacaaaaca tgaaatgaga aatgaaatta
      901 atgttcttac cttcatgtta agttgaagta gatcataatt atcccatgtg atttggtacc
      961 aaagtttttt ttttcagatc tacgtacact cgagtcgacc tttgctatga actcaaatat
     1021 gatccaataa aattttatat tcttctgtgt tttttttttt tgtattcaaa tttggataga
     1081 aacaagtatt tagtaggtcg taggtgccat aggtggccct tgggaaacat ccaatggata
     1141 caaacctcat attataaatt ctctatgcca aaataattag gaccataggt tatgttatgt
     1201 acctcaacca tcctttgatt gagacattaa attccttcac gtttgtaaac cacactttcg
     1261 tatacatttt cttccctaac acttcttggc actgtttctt ttattaattt gatttttgaa
     1321 acatatgtca aaagaaaaga aaagaatcga aacatttaat taattaatgt ttgtaattgc
     1381 acgttgtaca ttcattactt ttataaagag actgatcaaa tgatgattga taatcctttt
     1441 agtgtatatt aggcgtgtca atggtgtata tattatatag tgtctatatg tcataagaaa
     1501 cgtcataaat tttatcaaag gaacaacaat ttaagtctga ttaatctata attctcatcc
     1561 ttacattttc attggcatca aatttaggta atttttagaa tatagcttaa taaatgagta
     1621 ggccaagagg acggctcgca attagctgtg tggaaagcta aacgatgtct cctaccgtcc
     1681 acgtcagcgt cttaaatcct caaatcccat tggcaacaaa aaaaacattg tgtatataaa
     1741 tacatgcata cttatcactc tctactcact tttacaacta actcataagt aaaacacaga
     1801 cagtttttag aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata
     1861 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1921 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1981 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2041 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     2101 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat
     2161 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2221 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     2281 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2341 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg
     2401 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2461 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2521 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2581 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2641 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2701 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2761 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2821 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2881 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2941 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3001 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag
     3061 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3121 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3181 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
