LOCUS       sequence-495535-        2553 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2553)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30020
FEATURES             Location/Qualifiers
     source          1..2553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             202..219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(394..411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(565..1153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(645..664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1324..2115)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2337..2441)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2509..2527
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tgccgaatcc tgggggcatt accaatgttc cattcccaaa cctcccccag
       61 ttcaaagatc ttgctcgttt tgctgttcaa gattataata agaaagagaa tgctcatttg
      121 gagtttgtag aaaatttgaa tgtgaaggaa caagttgttg ctggaataat atactatata
      181 acgcttgtgg caactgatgc tcatcaccat caccatcacg gaaagaagaa aatatatgaa
      241 accaagattt tggtgaaggg atgggagaat ttcaaggaag ttcaagagtt caagcttgtt
      301 ggtgatgcca ctaaaatgtg agaccacgag tgattaatga atcggccaac gcgcggggag
      361 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      421 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      481 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      541 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      601 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      661 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      721 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      781 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      841 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      901 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      961 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1021 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1081 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1141 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1201 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1261 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1321 cagttatttg ccgactacct tggtgatctc gccttttacg taatggacaa attcttccaa
     1381 ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc
     1441 aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc
     1501 cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac
     1561 atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag
     1621 cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac
     1681 caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt
     1741 ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc
     1801 gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac
     1861 agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa
     1921 agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcgct
     1981 gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg
     2041 ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat
     2101 caccgcttcc ctcatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc
     2161 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     2221 ccgaggcata gactgtaccc caaaaaaaaa cagtcataac aagccatgaa aaccgccact
     2281 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatactc
     2341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2461 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2521 acgaggccct ttcgtccact gaagagccac tgt
//
