LOCUS       sequence-495536-        2295 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2295)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30023
FEATURES             Location/Qualifiers
     source          1..2295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..57
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(136..153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(307..895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(387..406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1066..1857)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2079..2183)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2251..2269
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tgaaagaaac cgctgctgct aaattcgaac gccagcacat ggactcaatg
       61 tgagaccacg agtgattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      121 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      181 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      241 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      301 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      361 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      421 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      481 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      541 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      601 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      661 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      721 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      781 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      841 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      901 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      961 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1021 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttatt tgccgactac
     1081 cttggtgatc tcgcctttta cgtaatggac aaattcttcc aactgatctg cgcgcgaggc
     1141 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
     1201 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     1261 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     1321 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     1381 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     1441 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     1501 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     1561 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     1621 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     1681 atcaagcctt acggtcaccg taaccagcaa atcaatatcg ctgtgtggct tcaggccgcc
     1741 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     1801 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgat
     1861 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
     1921 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
     1981 cccaaaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     2041 cgttcggtca aggttctgga ccagttgcgt gagcgcatac tcttcctttt tcaatattat
     2101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     2221 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtcca
     2281 ctgaagagcc actgt
//
