LOCUS       sequence-495539-        3490 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part SlUbi10 promoter, GGAG - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3490)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL12046
FEATURES             Location/Qualifiers
     source          1..3490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2117..2135)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2203..2307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2529..3320
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 acataaattc tttttttgta atgctcaaat aaatttgagt aaaaaagaat gaaattgagt
      901 gatttttttt taatcataag aaaataaata attaatttca atataataaa acagtaatat
      961 aatttcataa atggaattca atacttacct cttagatata aaaaataaat ataaaaataa
     1021 agtgtttcta ataaacccgc aatttaaata aaatatttaa tattttcaat caaatttaaa
     1081 taattatatt aaaatatcgt agaaaaagag caatatataa tacaagaaag aagatttaag
     1141 tacaattatc aactattatt atactctaat tttgttatat ttaatttctt acggttaagg
     1201 tcatgttcac gataaactca aaatacgctg tatgaggaca tattttaaat tttaaccaat
     1261 aataaaacta agttattttt agtatatttt tttgtttaac gtgacttaat ttttcttttc
     1321 tagaggagcg tgtaagtgtc aacctcattc tcctaatttt cccaaccaca taaaaaaaaa
     1381 ataaaggtag cttttgcgtg ttgatttggt acactacacg tcattattac acgtgttttc
     1441 gtatgattgg ttaatccatg aggcggtttc ctctagagtc ggccatacca tctataaaat
     1501 aaagctttct gcagctcatt ttttcatctt ctatctgatt tctattataa tttctctgaa
     1561 ttgccttcaa atttctcttt caaggttaga atttttctct attttttggt ttttgtttgt
     1621 ttagattctg agtttagtta atcaggtgct gttaaagccc taaattttga gtttttttcg
     1681 gttgttttga tggaaaatac ctaacaattg agttttttca tgttgttttg tcggagaatg
     1741 cctacaattg gagttccttt cgttgttttg atgagaaagc ccctaatttg agtgtttttc
     1801 cgtcgatttg attttaaagg tttatattcg agtttttttc gtcggtttaa tgagaaggcc
     1861 taaaatagga gtttttctgg ttgatttgac taaaaaagcc atggaatttt gtgtttttga
     1921 tgtcgctttg gttctcaagg cctaagatct gagtttctcc ggttgttttg atgaaaaagc
     1981 cctaaaattg gagtttttat cttgtgtttt aggttgtttt aatccttata atttgagttt
     2041 tttcgttgtt ctgattgttg tttttatgaa ttttgcaaat gtgagaccac gaagtggctc
     2101 ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2161 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2221 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2281 cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc
     2341 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     2401 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2461 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     2521 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2581 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2641 tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc
     2701 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2761 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2821 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2881 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2941 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     3001 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     3061 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     3121 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3181 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3241 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca
     3301 ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt
     3361 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3421 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3481 aaggatcttc
//
