LOCUS       sequence-495545-        2277 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2277)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30044
FEATURES             Location/Qualifiers
     source          1..2277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(118..135)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(289..877)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(369..388)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1048..1839)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2061..2165)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2233..2251
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggagcttgc tcttaagctt gctggactaa tgtgagacca cgagtgatta
       61 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      121 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      181 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      241 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      301 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      361 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      421 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      481 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      541 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      601 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      661 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      721 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      781 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      841 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      901 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      961 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1021 tatatatgag taaacttggt ctgacagtta tttgccgact accttggtga tctcgccttt
     1081 tacgtaatgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     1141 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     1201 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     1261 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     1321 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     1381 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     1441 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     1501 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     1561 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     1621 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     1681 cgtaaccagc aaatcaatat cgctgtgtgg cttcaggccg ccatccactg cggagccgta
     1741 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     1801 tagttgagtc gatacttcgg cgatcaccgc ttccctcatg atgtttaact ttgttttagg
     1861 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     1921 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aaaacagtca
     1981 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     2041 gaccagttgc gtgagcgcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2161 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     2221 ttaacctata aaaataggcg tatcacgagg ccctttcgtc cactgaagag ccactgt
//
