LOCUS       sequence-495547-        2400 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2400)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30048
FEATURES             Location/Qualifiers
     source          1..2400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             139..162
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(241..258)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(412..1000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(492..511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1171..1962)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2184..2288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2356..2374
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggatgtatt caagaaagga ttttcaatag ccgatgaggg agttgtggga
       61 gctgttgaga aaaccgatca gggtgtgaca gaagcagcag aaaagacaaa agagggtgtt
      121 atgagcagcg gtagcggtct ggaagttctg tttcagggcc caatgtgaga ccacgagtga
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      841 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1141 aagtatatat gagtaaactt ggtctgacag ttatttgccg actaccttgg tgatctcgcc
     1201 ttttacgtaa tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
     1261 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
     1321 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
     1381 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
     1441 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
     1501 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
     1561 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
     1621 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
     1681 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
     1741 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
     1801 caccgtaacc agcaaatcaa tatcgctgtg tggcttcagg ccgccatcca ctgcggagcc
     1861 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
     1921 tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta actttgtttt
     1981 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     2041 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaaaacag
     2101 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     2161 ctggaccagt tgcgtgagcg catactcttc ctttttcaat attattgaag catttatcag
     2221 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2281 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2341 acattaacct ataaaaatag gcgtatcacg aggccctttc gtccactgaa gagccactgt
//
