LOCUS       sequence-495549-        3580 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part Ubi10 promoter, GGAG - CCAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3580)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL13005
FEATURES             Location/Qualifiers
     source          1..3580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2207..2225)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2293..2397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2619..3410
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 gtcgagctgc aggtcaacgg atcaggatat tcttgtttaa gatgttgaac tctatggagg
      901 tttgtatgaa ctgatgatct aggaccggat aagttccctt cttcatagcg aacttattca
      961 aagaatgttt tgtgtatcat tcttgttaca ttgttattaa tgaaaaaata ttattggtca
     1021 ttggactgaa cacgagtgtt aaatatggac caggccccaa ataagatcca ttgatatatg
     1081 aattaaataa caagaataaa tcgagtcacc aaaccacttg ccttttttaa cgagacttgt
     1141 tcaccaactt gatacaaaag tcattatcct atgcaaatca ataatcatac aaaaatatcc
     1201 aataacacta aaaaattaaa agaaatggat aatttcacaa tatgttatac gataaagaag
     1261 ttacttttcc aagaaattca ctgattttat aagcccactt gcattagata aatggcaaaa
     1321 aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta gaaaatacga aatacgcttc
     1381 aatgcagtgg gacccacggt tcaattattg ccaattttca gctccaccgt atatttaaaa
     1441 aataaaacga taatgctaaa aaaatataaa tcgtaacgat cgttaaatct caacggctgg
     1501 atcttatgac gaccgttaga aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa
     1561 gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct
     1621 caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca
     1681 cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc
     1741 ctcgtctttt cttcttcttc ttctataaaa caatacccaa agagctcttc ttcttcacaa
     1801 ttcagatttc aatttctcaa aatcttaaaa actttctctc aattctctct accgtgatca
     1861 aggtaaattt ctgtgttcct tattctctca aaatcttcga ttttgttttc gttcgatccc
     1921 aatttcgtat atgttctttg gtttagattc tgttaatctt agatcgaaca cgattttctg
     1981 ggtttgatcg ttagatatca tcttaattct cgattagggt ttcatagata tcatccgatt
     2041 tgttcaaata atttgagttt tgtcgaataa ttactcttcg atttgtgatt tctatctaga
     2101 tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag tttttctgat
     2161 taacaggcca ttgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc
     2221 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2401 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2461 gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca
     2521 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     2581 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg
     2641 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     2701 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata
     2761 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     2821 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     2881 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     2941 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     3001 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     3061 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     3121 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     3181 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     3241 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     3301 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     3361 aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc
     3421 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3481 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3541 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
