LOCUS       sequence-495550-        3677 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part Long 35s gene cauliflower mosaic virus and 
            5 prime untranslated leader tobacco mosaic virus promoter, GGAG - 
            CCAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3677)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL13008
FEATURES             Location/Qualifiers
     source          1..3677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1830..2175
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2118..2138
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(2304..2322)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2390..2494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2716..3507
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 gaattccaat cccacaaaaa tctgagctta acagcacagt tgctcctctc agagcagaat
      901 cgggtattca acaccctcat atcaactact acgttgtgta taacggtcca catgccggta
      961 tatacgatga ctggggttgt acaaaggcgg caacaaacgg cgttcccgga gttgcacaca
     1021 agaaatttgc cactattaca gaggcaagag cagcagctga cgcgtacaca acaagtcagc
     1081 aaacagacag gttgaacttc atccccaaag gagaagctca actcaagccc aagagctttg
     1141 ctaaggccct aacaagccca ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa
     1201 ggcccagcag tgatccagcc ccaaaagaga tctcctttgc cccggagatt acaatggacg
     1261 atttcctcta tctttacgat ctaggaagga agttcgaagg tgaaggtgac gacactatgt
     1321 tcaccactga taatgagaag gttagcctct tcaatttcag aaagaatgct gacccacaga
     1381 tggttagaga ggcctacgca gcaagtctca tcaagacgat ctacccgagt aacaatctcc
     1441 aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaatt
     1501 gcatcaagaa cacagagaaa gacatatttc tcaagatcag aagtactatt ccagtatgga
     1561 cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaaaaagg
     1621 tagttcctac tgaatctaag gccatgcatg gagtctaaga ttcaaatcga ggatctaaca
     1681 gaactcgccg tcaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag
     1741 aagaaaatct tcgtcaacat ggtggagcac gacactctgg tctactccaa aaatgtcaaa
     1801 gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat aatttcggga
     1861 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
     1921 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatctc
     1981 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     2041 gaggttccaa ccacgtctac aaagcaagtg gattgatgtg acatctccac tgacgtaagg
     2101 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     2161 catttggaga ggacacgctc gagtataaga gctcattttt acaacaatta ccaacaacaa
     2221 caaacaacaa acaacattac aattacattt acaattatcg atacccattg agaccacgaa
     2281 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     2341 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2401 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2461 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
     2521 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2581 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2641 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2701 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2761 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2821 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
     2881 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2941 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     3001 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     3061 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     3121 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     3181 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     3241 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     3301 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     3361 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     3421 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     3481 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     3541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3661 aagatcaaag gatcttc
//
