LOCUS       sequence-495554-        4241 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part Ubiquitin (ZmUbi) promoter, GGAG - CCAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4241)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL13016A
FEATURES             Location/Qualifiers
     source          1..4241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2868..2886)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2954..3058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3280..4071
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
      901 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
      961 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
     1021 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
     1081 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
     1141 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
     1201 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
     1261 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
     1321 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
     1381 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
     1441 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
     1501 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
     1561 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
     1621 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
     1681 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
     1741 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
     1801 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     1861 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1921 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1981 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     2041 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     2101 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     2161 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     2221 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     2281 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     2341 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     2401 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     2461 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     2521 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     2581 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     2641 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     2701 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     2761 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     2821 tctgcaggcc attgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg
     2881 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2941 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3001 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3061 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     3121 ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc
     3181 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     3241 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
     3301 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     3361 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat
     3421 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     3481 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     3541 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     3601 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     3661 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     3721 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     3781 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     3841 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     3901 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     3961 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     4021 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
     4081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
