LOCUS       sequence-495559-        2544 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2544)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30060
FEATURES             Location/Qualifiers
     source          1..2544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             28..189
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     primer_bind     complement(385..402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(556..1144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(636..655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1315..2106)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2328..2432)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2500..2518
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggcaaagta tcgccagtac aaactgatcc tgaacggtaa aaccctgaaa
       61 ggtgaaacca ccaccgaagc tgttgacgct gctaccgcgg aaaaagtttt caaacagtac
      121 gctaacgaca acggtgttga cggtgaatgg acctacgacg acgctaccaa aaccttcacc
      181 gttaccgaaa gcagcggcgt gaaaattgtg agccgcaaaa gcctgggcgt gcagaatgtg
      241 tatgatattg gcgtggaaaa agatcataac tttctgctgg cgaatggcct gattgcgagc
      301 aacggaatgt gagaccacga gtgattaatg aatcggccaa cgcgcgggga gaggcggttt
      361 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      421 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      481 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      541 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      601 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      661 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      721 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      781 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      841 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      901 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      961 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1021 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1081 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1141 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1201 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1261 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttattt
     1321 gccgactacc ttggtgatct cgccttttac gtaatggaca aattcttcca actgatctgc
     1381 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac
     1441 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc
     1501 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc
     1561 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa
     1621 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac
     1681 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc
     1741 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc
     1801 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag
     1861 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg
     1921 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcgc tgtgtggctt
     1981 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg
     2041 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc
     2101 cctcatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
     2161 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     2221 agactgtacc ccaaaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2281 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatact cttccttttt
     2341 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2401 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2461 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     2521 tttcgtccac tgaagagcca ctgt
//
