LOCUS       sequence-495561-        2895 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part RbcS terminator, GCTT - CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2895)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60006
FEATURES             Location/Qualifiers
     source          1..2895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      841..1482
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(1522..1540)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1608..1712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1934..2725
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat
      901 tgcgcacaca ccagaatcct actgagtttg agtattatgg cattgggaaa actgtttttc
      961 ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac
     1021 tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc
     1081 aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag
     1141 atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag
     1201 ttaatatgag gagtaaaaca cttgtagttg taccattatg cttattcact aggcaacaaa
     1261 tatattttca gacctagaaa agctgcaaat gttactgaat acaagtatgt cctcttgtgt
     1321 tttagacatt tatgaacttt cctttatgta attttccaga atccttgtca gattctaatc
     1381 attgctttat aattatagtt atactcatgg atttgtagtt gagtatgaaa atatttttta
     1441 atgcatttta tgacttgcca attgattgac aacatgcatc aacgcttgag accacgaagt
     1501 ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1561 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1621 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1681 aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct
     1741 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     1801 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     1861 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     1921 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     1981 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     2041 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
     2101 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     2161 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     2221 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     2281 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     2341 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     2401 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     2461 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     2521 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     2581 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     2641 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     2701 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     2761 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2821 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2881 gatcaaagga tcttc
//
