LOCUS       sequence-495563-        2934 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part HSP18 terminator, GCTT - CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2934)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60008
FEATURES             Location/Qualifiers
     source          1..2934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      844..1092
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(1561..1579)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1647..1751
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1973..2764
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 gaatatgaag atgaagatga aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt
      901 ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa tattaaatga
      961 atgtaagatc tcattataat gaataaacaa atgtttctat aatccattgt gaatgttttg
     1021 ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact atggaatatg
     1081 attaaagata agatgggctc atagagtaaa acgaggcgag ggacctataa acctcccttc
     1141 atcatgctat ttcatgatct attttataaa ataaagatgt agaaaaaagt aagcgtaata
     1201 accgcaaaac aaatgattta aaacatggca cataatgagg agattaagtt cggtttacgt
     1261 ttattttagt actaattgta acgtgagact acgtatcggg aatcgcctaa ttaaagcatt
     1321 aatgcgaacc tgattagatt caccgaccct cctatcgtgt cgacctttct gtttcttaga
     1381 attttttggt agtctatgta ctaataatgt cagcttcgta tttatttcat aagcaatttg
     1441 catttgcaat ttgtttttta cttttatttt tattgtattg tggaatgtgg actcgtacca
     1501 acatgaagtt atataccacc acgcttgaga ccacgaagtg gctcttcagt ggacgaaagg
     1561 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1621 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1681 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1741 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1801 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat
     1861 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     1921 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
     1981 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     2041 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     2101 gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     2161 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     2221 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     2281 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     2341 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     2401 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     2461 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     2521 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     2581 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     2641 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     2701 agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa
     2761 ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2821 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2881 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
