LOCUS       sequence-495564-        3015 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part TrpC terminator, GCTT - CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3015)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60013
FEATURES             Location/Qualifiers
     source          1..3015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1642..1660)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1728..1832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2054..2845
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 cgggggatga aatcatcaaa cagcttgacg aatctggata taagatcgtt ggtgtcgatg
      901 tcagctccgg agttgagaca aatggtgttc aggatctcga taagatacgt tcatttgtcc
      961 aagcagcaaa gagtgccttc tagtgattta atagctccat gtcaacaaga ataaaacgcg
     1021 tttcgggttt acctcttcca gatacagctc atctgcaatg cattaatgca ttggacctcg
     1081 caaccctagt acgcccttca ggctccggcg aagcagaaga atagcttagc agagtctatt
     1141 ttcattttcg ggagacgaga tcaagcagat caacggtcgt caagagaact acgagactga
     1201 ggaatccgct cttggctcca cgcgactata tatttgtctc taattgtact ttgacatgct
     1261 cctcttcttt actctgatag cttgactatg aaaattccgt caccagcccc tgggttcgca
     1321 aagataattg cactgtttct tccttgaact ctcaagccta caggacacac attcatcgta
     1381 ggtataaacc tcgaaaatca ttcctactaa gatgggtata caatagtaac catgcatggt
     1441 tgcctagtga atgctccgta acacccaata cgccggccga aactttttta caactctcct
     1501 atgagtcgtt tacccagaat gcacaggtac acttgtttag aggtaatcct tctttctaga
     1561 agtcctcgtg tactgtgtaa gcgcccactc cacatctcca ctcgcttgag accacgaagt
     1621 ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1681 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1801 aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct
     1861 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     1921 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     1981 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     2041 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     2101 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     2161 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
     2221 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     2281 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     2341 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     2401 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     2461 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     2521 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     2581 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     2641 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     2701 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     2761 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     2821 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     2881 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2941 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3001 gatcaaagga tcttc
//
