LOCUS       sequence-495568-        2358 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2358)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30072
FEATURES             Location/Qualifiers
     source          1..2358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             97..120
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(199..216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(370..958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(450..469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1129..1920)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2142..2246)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2314..2332
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggcacacca tcaccaccat cacagcagcg gtggactgaa tgatatattc
       61 gaggctcaga agattgaata ccacgagagc agcggtctgg aagttctgtt tcagggccca
      121 atgtgagacc acgagtgatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      181 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      241 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      301 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      361 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      421 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      481 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      541 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      601 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      661 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      721 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      781 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      841 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      901 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      961 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1021 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1081 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt atttgccgac
     1141 taccttggtg atctcgcctt ttacgtaatg gacaaattct tccaactgat ctgcgcgcga
     1201 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     1261 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     1321 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     1381 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     1441 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     1501 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     1561 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     1621 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     1681 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     1741 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcgctgtgtg gcttcaggcc
     1801 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     1861 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
     1921 gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
     1981 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
     2041 taccccaaaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     2101 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tactcttcct ttttcaatat
     2161 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2221 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2281 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     2341 ccactgaaga gccactgt
//
