LOCUS       sequence-495569-        2547 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2547)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30073
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..105
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             115..276
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             286..309
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(388..405)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(559..1147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(639..658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1318..2109)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2331..2435)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2503..2521
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tgcatcatca ccaccatcac agcagcggtg gactgaatga tatattcgag
       61 gctcagaaga ttgaatacca cgagagcagc ggcatcgaag gccgcggccg ccagtacaaa
      121 ctgatcctga acggtaaaac cctgaaaggt gaaaccacca ccgaagctgt tgacgctgct
      181 accgcggaaa aagttttcaa acagtacgct aacgacaacg gtgttgacgg tgaatggacc
      241 tacgacgacg ctaccaaaac cttcaccgtt accgaaagca gcggcctgga agttctgttt
      301 cagggcccaa tgtgagacca cgagtgatta atgaatcggc caacgcgcgg ggagaggcgg
      361 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      421 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      481 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      961 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1321 tttgccgact accttggtga tctcgccttt tacgtaatgg acaaattctt ccaactgatc
     1381 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
     1441 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
     1501 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
     1561 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
     1621 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
     1681 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
     1741 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
     1801 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
     1861 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
     1921 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cgctgtgtgg
     1981 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
     2041 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
     2101 ttccctcatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
     2161 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
     2221 catagactgt accccaaaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     2281 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat actcttcctt
     2341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2461 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2521 ccctttcgtc cactgaagag ccactgt
//
