LOCUS       sequence-495572-        2254 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2254)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50028
FEATURES             Location/Qualifiers
     source          1..2254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(54..72)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        140..244
                     /gene="bla"
                     /label=AmpR promoter
     CDS             466..1257
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1428..2016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1917..1936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2170..2187
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gtgataggct ttgagaccac gtggctcttc agtggacgaa agggcctcgt
       61 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      121 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      181 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      241 gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
      301 agtggcggtt ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg
      361 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
      421 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg
      481 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
      541 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
      601 agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
      661 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
      721 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
      781 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
      841 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
      901 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
      961 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     1021 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     1081 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     1141 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     1201 ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg
     1261 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1321 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1561 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1681 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1741 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1801 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1861 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1921 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1981 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2041 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2101 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2161 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     2221 ctccccgcgc gttggccgat tcattaatca ctgt
//
