LOCUS       sequence-495573-        2296 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2296)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50029
FEATURES             Location/Qualifiers
     source          1..2296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..56
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(96..114)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        182..286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             508..1299
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1470..2058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1959..1978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2212..2229
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gaaagaaacc gctgctgcta aattcgaacg ccagcacatg gacagctagg
       61 ctttgagacc acgtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
      121 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      181 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      241 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
      301 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      361 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
      421 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
      481 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
      541 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
      601 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
      661 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
      721 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
      781 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
      841 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
      901 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
      961 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     1021 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     1081 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     1141 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     1201 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat
     1261 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca
     1321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1381 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2161 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2221 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2281 attcattaat cactgt
//
