LOCUS       sequence-495575-        3130 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3130)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50038
FEATURES             Location/Qualifiers
     source          1..3130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             48..80
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             99..869
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     primer_bind     complement(930..948)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1016..1120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1342..2133
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2304..2892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2793..2812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3046..3063
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gggaggcggt tcaggtggcg gatcgggcgg tggatcaatg gctagcatga
       61 ctggtggaca gcaaatgggt cgcggatccg aattcatgat cccgctgctg aacgctaaac
      121 agattctggg acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca
      181 atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat
      241 atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc
      301 tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg
      361 tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca
      421 aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg
      481 ccggaataac aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga
      541 ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta
      601 acctggacag gaatactctg gccgctatgc tgatccgaga gctgcgggca gccctggaac
      661 tgttcgagca ggaaggcctg gctccatatc tgtcacggtg ggagaagctg gataacttca
      721 tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga
      781 ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg
      841 gagaaatcag tctgcggtct gccgaaaaga agcttgcggc cgcactcgag tgagctttga
      901 gaccacgtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      961 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1021 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1081 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1141 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1201 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1261 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1321 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1381 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1441 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     1501 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     1561 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     1621 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     1681 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     1741 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     1801 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     1861 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     1921 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     1981 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2041 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     2101 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     2161 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2221 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2281 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2341 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2401 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2461 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2521 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2581 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2641 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2701 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2761 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2821 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2881 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2941 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3001 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3061 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3121 taatcactgt
//
