LOCUS       sequence-495576-        4261 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4261)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50039
FEATURES             Location/Qualifiers
     source          1..4261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2061..2079)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2147..2251
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2473..3264
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3435..4023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3924..3943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4177..4194
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gggtcagtcc cttatgttac gtcctgtaga aaccccaacc cgtgaaatca
       61 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
      121 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
      181 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
      241 tctttatacc gaaaggtaag tcttactctc tcttttttgg tctgtatttt taattttttg
      301 aagtatacta tttgtactga cgctaataat cttttttcag gttgggcagg ccagcgtatc
      361 gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt gggtcaataa tcaggaagtg
      421 atggagcatc agggcggcta tacgccattt gaagccgatg tcacgccgta tgttattgcc
      481 gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg
      541 ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc
      601 tttaactatg ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg
      661 gacgatatca ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg
      721 caggtacttc atgcttcaac gtgtaactta agagatactg tgtgaaattt tatatttcca
      781 tacatttgct tgacctttgc tttttgtcaa tttttttccc cttacaggtg gtggccaatg
      841 gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca
      901 ctagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct
      961 atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg cttcgcgtcg
     1021 gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa ccgttctact
     1081 ttactggctt tggtcgtcat gaagatgcgg acttgcgtgg caaaggattc gataacgtgc
     1141 tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac cgtacctcgc
     1201 attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc gtggtgattg
     1261 atgaaactgc tgctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca
     1321 agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa gcgcacttac
     1381 aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta
     1441 ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg ccactggcgg
     1501 aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg
     1561 acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg
     1621 gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaacttc
     1681 tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc gtggatacgt
     1741 tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc
     1801 tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga
     1861 atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga
     1921 tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg
     1981 gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgagctttg agaccacgtg
     2041 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     2101 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2161 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2221 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
     2281 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     2341 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     2401 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     2461 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     2521 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2581 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     2641 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     2701 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2761 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     2821 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     2881 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     2941 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     3001 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     3061 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     3121 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     3181 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     3241 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     3301 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3361 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3421 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3481 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3541 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3601 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3661 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3721 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3781 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3841 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3901 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3961 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4021 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4081 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4141 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4201 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactg
     4261 t
//
