LOCUS       sequence-495579-        3286 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3286)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50042
FEATURES             Location/Qualifiers
     source          1..3286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             39..998
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             1005..1046
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(1086..1104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1172..1276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1498..2289
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2460..3048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2949..2968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3202..3219
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gatggctcgg gatccaccgg tcgccaccat gaaagacaat actgtgcctc
       61 tgaagctgat cgctctcctg gctaatggcg agttccatag tggcgaacag ctgggagaaa
      121 ccctgggcat gtccagggcc gctatcaaca agcacattca gactctgcgc gactggggcg
      181 tggacgtgtt caccgtgccc ggaaagggct actctctgcc cgagcctatc ccgctgctga
      241 acgctaaaca gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg
      301 actccaccaa tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca
      361 ttgcagaata tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg
      421 gagctaacct gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc
      481 tgggcccggt catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg
      541 tgcgagtcaa atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg
      601 tggagctggc cggaataaca ggcgatgctg cacagatcgt cattggcgcc gggattaacg
      661 tggctatgag gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag
      721 cagggattaa cctggacagg aatactctgg ccgctacgct gatccgagag ctgcgggcag
      781 ccctggaact gttcgagcag gaaggcctgg ctccatatct gccacggtgg gagaagctgg
      841 ataacttcat caatagaccc gtgaagctga tcattgggga caaagagatt ttcgggatta
      901 gccgggggat tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct
      961 ggatgggcgg agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca
     1021 accccctgct gggcctggac agcacctaag ctttgagacc acgtggctct tcagtggacg
     1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1261 ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     1321 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttt tggggtacag
     1381 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     1441 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     1501 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     1561 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     1621 ctgaagccac acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     1681 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     1741 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     1801 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     1861 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     1921 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     1981 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2041 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2101 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2161 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     2221 cgcgcagatc agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc
     2281 ggcaaataac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2341 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2401 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2461 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2521 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2581 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2641 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2701 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2761 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2821 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2881 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2941 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3001 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3061 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3181 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3241 cgcaaaccgc ctctccccgc gcgttggccg attcattaat cactgt
//
