LOCUS       sequence-495583-        3390 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3390)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50056
FEATURES             Location/Qualifiers
     source          1..3390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1109..1150
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(1190..1208)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1276..1380
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1602..2393
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2564..3152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3053..3072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3306..3323
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gatggctcgg gatccaccgg tcgccaccat gaaagacaat actgtgcctc
       61 tgaagctgat cgctctcctg gctaatggcg agttccatag tggcgaacag ctgggagaaa
      121 ccctgggcat gtccagggcc gctatcaaca agcacattca gactctgcgc gactggggcg
      181 tggacgtgtt caccgtgccc ggaaagggct actctctgcc cgagcctatc ccgctgctga
      241 acgctaaaca gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg
      301 actccaccaa tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca
      361 ttgcagaata tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg
      421 gagctaacct gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc
      481 tgggcccggt catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg
      541 tacttcatgc ttcaacgtgt aacttaagag atactgtgtg aaattttata tttccataca
      601 tttgcttgac ctttgctttt tgtcaatttt tttcccctta caggtgcgag tcaaatggcc
      661 caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat
      721 aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt
      781 ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga
      841 caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg aactgttcga
      901 gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact tcatcaatag
      961 acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa
     1021 acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat
     1081 cagtctgcgg tctgccgaaa aggctagcgg caagcccatc cccaaccccc tgctgggcct
     1141 ggacagcacc taagctttga gaccacgtgg ctcttcagtg gacgaaaggg cctcgtgata
     1201 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1261 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1321 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1381 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     1441 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat
     1501 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1561 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     1621 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     1681 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg
     1741 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     1801 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     1861 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     1921 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     1981 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2041 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2101 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2161 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2221 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2281 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2341 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag
     2401 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2461 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2521 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2581 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2641 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2701 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2761 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2821 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2881 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2941 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3001 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3061 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3121 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3181 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3241 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3301 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3361 ccgcgcgttg gccgattcat taatcactgt
//
