LOCUS       sequence-495590-        6286 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Expression of Wild Type Human GLP1R, Cre dependent, under hSyn 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Godschall EN, Gungul TB, Sajonia IR, Buyukaksakal AK, Li OD, Ogilvie
            S, Keeler AB, Tian G, Koita O, Shi Y, Deutsch TCJ, Crook M, Zhang Y,
            Conley NJ, Webster AN, Calhan OY, Liu W, Akkoub A, Malik K, West KI,
            Michel-Le S, Karthikeyan A, van Gerven G, Beier KT, Zweifel LS, 
            Patel MK, Campbell JN, Deppmann CD, Guler AD
  TITLE     A Brain Reward Circuit Inhibited By Next-Generation Weight Loss 
            Drugs.
  JOURNAL   bioRxiv [Preprint]. 2025 Jun 14:2024.12.12.628169. doi: 
            10.1101/2024.12.12.628169.
  PUBMED    39763823
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jun 14:2024.12.12.628169. doi: 
            10.1101/2024.12.12.628169."
COMMENT     Sequence Label: AG0053 LGA (DIO-hGLP1R)
FEATURES             Location/Qualifiers
     source          1..6286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    complement(660..693)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(744..777)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2271..2304
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    2355..2388
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2413..3001
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2466..2486)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2884..2895)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3033..3509
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3232..3251)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3549..3689
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3549..3678
                     /label=AAV2 ITR
     rep_origin      3764..4219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3851..3870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4061..4082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4236..4255)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4355..4377
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4415..4433)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4501..4605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4606..4674,4675..5466)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4824..4843)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5637..6225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6126..6145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc ctctagagtc gactccggaa
      661 taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga ttgtagctgc
      721 tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg aagttatggc
      781 gcgccctaag agcaggacgc ctgacaagtg gctgtataca ttgaagagcc ggcggtggct
      841 ccggaactca ggctggaagt gggacattta agtggcttca tagaactatc gcgctgaata
      901 tggaggtgtt caagccgcca ccgttcccat gacttccgaa attcaagttg gacctcattg
      961 ttcacgaagc agtagagaat agccaccatc aatccttgga aactagtgaa agacagctct
     1021 gtgaacagtt tgataaaccg cagtgtgcct ctcgcatgct cgtccatcac gaaggcaaat
     1081 atcacttcat gtgttccgag cagcgggatg agggtgagag tggacttagc cagtctgcac
     1141 ttaatgtcgg ttttgcacat aaggttagct ttcagctttg acacgacgat acagatcact
     1201 ctcacaaata taagaaagtt gactccaatc gcgaagagta tgggcaggcg tatgatgagc
     1261 cagtagttca tgttagagtt tctggtccaa cagccttcat cttcatacag gtacttgacg
     1321 atgccccaag gcaccacgaa cagcagaggc acaccccatc caatggacac gtaaagcctg
     1381 aaaatccact gttcactcag cacggagaat gcgagcagtg tgtaaagata tactccttcg
     1441 accagcagcc aataataatt ggctgccaca cagtactgca taagcagaaa cacgagtcga
     1501 caggaaagag aatcctgata gctgaggagt ccatcccatt ggtgctgttg ggccgcggta
     1561 gagtacatcc acttcagggc agcgtcctta atgaacacac tcaaggcccg cagaataaaa
     1621 cttgcaaaga gattcaagtg gatgtaattt cttgtgcagt gaaggtggcg aaaccccaaa
     1681 aggatggcac tagcgataac cagagcggaa aaggacagcg cgtagccgac ggtgtagata
     1741 atgtacagaa acagcagctg ttcctcgggg gaactgcgct cgcccctctt agattcctcg
     1801 cactcgctaa gatcgcgcca tggaagggaa ctgttatctt tttgcagcca gaggccctca
     1861 gctgtgcaga acctgtagac gtgtccttga ggcacagatg atgcccatgg caggtaccat
     1921 ggacatgaca cattcacgaa tgagccgggc tcgccgtctg gccagcaggc atactcatca
     1981 aatgtcctat tgcaaaacaa gtcggtggct ggaggaggat cttctgtcag gctgcgttga
     2041 cactggcggc gatactcccg ccacttctgg acggtctccc aaagactcac ggtagcgccc
     2101 tgaggtctgg gtccggctcg tccgaccatc cccagaagca gcagtgcgag cctgaggggt
     2161 ccaggggcac ctgccatatc gatgctggcg tcatcatcgt ccttgtagtc ggcgaatacc
     2221 aggcagaaga tgtagctcag ggcgatgatc gtcttcatgg tggcgctagc ataacttcgt
     2281 ataaagtatc ctatacgaag ttatttgcct taacccagaa attatcactg ttattcttta
     2341 gaatggtgca aagaataact tcgtataatg tatgctatac gaagttatga attcgatatc
     2401 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2461 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2521 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2581 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2641 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2701 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2761 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     2821 ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2881 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2941 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3001 catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag
     3061 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     3121 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     3181 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     3241 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     3301 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     3361 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     3421 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     3481 cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga
     3541 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     3601 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     3661 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     3721 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     3781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3841 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3901 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3961 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4021 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4081 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4141 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4201 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4261 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4321 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4381 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4441 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4501 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4561 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4621 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4681 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4741 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4801 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4861 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4921 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4981 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5041 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5101 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5161 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5221 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5281 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5341 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5401 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5461 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5521 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5581 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5641 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5701 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5761 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5821 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5881 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5941 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6001 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6061 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6121 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6181 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6241 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
