LOCUS       sequence-49560-e         546 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Kwon GS, Hadjantonakis AK
  TITLE     Transthyretin mouse transgenes direct RFP expression or Cre-mediated
            recombination throughout the visceral endoderm.
  JOURNAL   Genesis. 2009 Jul;47(7):447-55.
  PUBMED    19415627
REFERENCE   2  (bases 1 to 546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis."; 
            date: "2009-07"; volume: "47(7)"; pages: "447-55"
COMMENT     Sequence Label: Ttr:mRFP1
FEATURES             Location/Qualifiers
     source          1..546
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    243..377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(280..299)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     334..353
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(411..429)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(412..429)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 acgagcgcgc cgagggccgc cactccaccg gcgcctaaga attcctaatg tcctctgatg
       61 gtcaaagtcc tggatgctgt ccgaggcagc cctgctgtag acgtggctgt aaaagtgttc
      121 aaaaagacct ctgagggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      181 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      241 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      301 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      361 cttatcatgt ctggatcctg ggagcccttt gcctctgggt aagcttgtat tctatagtgt
      421 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
      481 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
      541 ctctcg
//
