LOCUS       sequence-495601-        9109 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Lentiviral expression vector for BMP2 with V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Marohl T, Atkins KA, Wang L, Janes KA
  TITLE     PCSK5M452I is a recessive hypomorph exclusive to MCF10DCIS.com 
            cells.
  JOURNAL   Mol Cancer Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211.
  PUBMED    41148168
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211."
COMMENT     Sequence Label: pLX302 BMP2-V5 puro
FEATURES             Location/Qualifiers
     source          1..9109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..510
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    635..752
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        869..1172
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1173..1376
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1327..1347
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1399..1423
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1424..1433
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2615..2639)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2641..2682
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2724..3312
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2777..2797)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3195..3206)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3384..3617
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3695..3816
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3732..3751)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3786..3805
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3856..3991
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4011..4030)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4012..4030)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4040..4057)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4040..4056)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4049..4071)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4198..4653
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4285..4304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4495..4516
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4679..4783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4784..4852,4853..5644)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5002..5021)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5815..6403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6304..6323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6557..6574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6691..6712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6727..6732,6733..6750,6751..6757)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6765..6781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6770..6792
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6789..6805
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6789..6805
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6824..6844
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6826..6844
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6872..7098
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7099..7279
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7326..7451
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7944..8177
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8362..8406
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8581..9081
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9000..9018
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(9103..9109,1..593)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(join(9103..9109,1..13))
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
       61 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
      121 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
      181 aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg
      241 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
      301 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
      361 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
      421 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
      481 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
      541 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagaattct
      601 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
      661 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      721 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      781 cactttggcg ccggctcgag ggggcccggg gttattaata gtaatcaatt acggggtcat
      841 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      901 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      961 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     1021 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1081 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1141 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     1201 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1261 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     1321 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     1381 ggctaactgt cgggatcaac aagtttgtac aaaaaagttg gcaccatggt ggccgggacc
     1441 cgctgtcttc tagcgttgct gcttccccag gtcctcctgg gcggcgcggc tggcctcgtt
     1501 ccggagctgg gccgcaggaa gttcgcggcg gcgtcgtcgg gccgcccctc atcccagccc
     1561 tctgacgagg tcctgagcga gttcgagttg cggctgctca gcatgttcgg cctgaaacag
     1621 agacccaccc ccagcaggga cgccgtggtg cccccctaca tgctagacct gtatcgcagg
     1681 cactcaggtc agccgggctc acccgcccca gaccaccggt tggagagggc agccagccga
     1741 gccaacactg tgcgcagctt ccaccatgaa gaatctttgg aagaactacc agaaacgagt
     1801 gggaaaacaa cccggagatt cttctttaat ttaagttcta tccccacgga ggagtttatc
     1861 acctcagcag agcttcaggt tttccgagaa cagatgcaag atgctttagg aaacaatagc
     1921 agtttccatc accgaattaa tatttatgaa atcataaaac ctgcaacagc caactcgaaa
     1981 ttccccgtga ccagactttt ggacaccagg ttggtgaatc agaatgcaag caggtgggaa
     2041 agttttgatg tcacccccgc tgtgatgcgg tggactgcac agggacacgc caaccatgga
     2101 ttcgtggtgg aagtggccca cttggaggag aaacaaggtg tctccaagag acatgttagg
     2161 ataagcaggt ctttgcacca agatgaacac agctggtcac agataaggcc attgctagta
     2221 acttttggcc atgatggaaa agggcatcct ctccacaaaa gagaaaaacg tcaagccaaa
     2281 cacaaacagc ggaaacgcct taagtccagc tgtaagagac accctttgta cgtggacttc
     2341 agtgacgtgg ggtggaatga ctggattgtg gctcccccgg ggtatcacgc cttttactgc
     2401 cacggagaat gcccttttcc tctggctgat catctgaact ccactaatca tgccattgtt
     2461 cagacgttgg tcaactctgt taactctaag attcctaagg catgctgtgt cccgacagaa
     2521 ctcagtgcta tctcgatgct gtaccttgac gagaatgaaa aggttgtatt aaagaactat
     2581 caggacatgg ttgtggaggg ttgtgggtgt cgcttgccaa ctttcttgta caaagtggtt
     2641 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct
     2701 aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     2761 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2821 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2881 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2941 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3001 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3061 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3121 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3181 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3241 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3301 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     3361 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     3421 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3481 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     3541 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3601 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     3661 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     3721 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3781 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     3841 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3901 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3961 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     4021 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     4081 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     4141 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     4201 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4261 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4321 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4381 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4441 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4501 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4561 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4621 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     4681 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4741 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4801 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4861 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4921 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4981 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5041 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5101 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5161 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5221 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5281 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5341 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5401 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5461 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5521 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5581 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5641 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5701 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5761 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5821 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5881 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5941 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6001 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6061 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6481 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6541 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6601 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     6661 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6721 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6781 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     6841 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     6901 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     6961 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     7021 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     7081 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7141 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7201 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7261 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     7321 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     7381 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     7441 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     7501 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     7561 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     7621 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     7681 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     7741 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     7801 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     7861 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7921 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     7981 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     8041 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8101 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8161 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8221 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     8281 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     8341 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     8401 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     8461 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     8521 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     8581 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     8641 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     8701 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     8761 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     8821 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     8881 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     8941 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     9001 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     9061 atcaccgacc tctctcccca gggggtacca ccggagctta ccatgaccg
//
