LOCUS       sequence-495602-        9142 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Lentiviral expression vector for BMP4 with V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Marohl T, Atkins KA, Wang L, Janes KA
  TITLE     PCSK5M452I is a recessive hypomorph exclusive to MCF10DCIS.com 
            cells.
  JOURNAL   Mol Cancer Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211.
  PUBMED    41148168
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211."
COMMENT     Sequence Label: pLX302 BMP4-V5 puro
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..510
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    635..752
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        869..1172
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1173..1376
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1327..1347
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1399..1423
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2648..2672)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2674..2715
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2757..3345
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2810..2830)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3228..3239)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3417..3650
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3728..3849
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3765..3784)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3819..3838
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3889..4024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4044..4063)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4045..4063)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4073..4090)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4073..4089)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4082..4104)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4231..4686
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4318..4337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4528..4549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4712..4816
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4817..4885,4886..5677)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5035..5054)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5848..6436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6337..6356
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6590..6607
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6724..6745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6760..6765,6766..6783,6784..6790)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6798..6814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6803..6825
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6822..6838
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6822..6838
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6857..6877
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6859..6877
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6905..7131
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7132..7312
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7359..7484
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7977..8210
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8395..8439
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8614..9114
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9033..9051
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(9136..9142,1..593)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(join(9136..9142,1..13))
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
       61 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
      121 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
      181 aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg
      241 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
      301 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
      361 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
      421 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
      481 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
      541 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagaattct
      601 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
      661 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      721 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      781 cactttggcg ccggctcgag ggggcccggg gttattaata gtaatcaatt acggggtcat
      841 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      901 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      961 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     1021 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1081 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1141 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     1201 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1261 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     1321 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     1381 ggctaactgt cgggatcaac aagtttgtac aaaaaagttg gcatgattcc tggtaaccga
     1441 atgctgatgg tcgttttatt atgccaagtc ctgctaggag gcgcgagcca tgctagtttg
     1501 atacctgaga cggggaagaa aaaagtcgcc gagattcagg gccacgcggg aggacgccgc
     1561 tcagggcaga gccatgagct cctgcgggac ttcgaggcga cacttctgca gatgtttggg
     1621 ctgcgccgcc gcccgcagcc tagcaagagt gccgtcattc cggactacat gcgggatctt
     1681 taccggcttc agtctgggga ggaggaggaa gagcagatcc acagcactgg tcttgagtat
     1741 cctgagcgcc cggccagccg ggccaacacc gtgaggagct tccaccacga agaacatctg
     1801 gagaacatcc cagggaccag tgaaaactct gcttttcgtt tcctctttaa cctcagcagc
     1861 atccctgaga acgaggcgat ctcctctgca gagcttcggc tcttccggga gcaggtggac
     1921 cagggccctg attgggaaag gggcttccac cgtataaaca tttatgaggt tatgaagccc
     1981 ccagcagaag tggtgcctgg gcacctcatc acacgactac tggacacgag actggtccac
     2041 cacaatgtga cacggtggga aacttttgat gtgagccctg cggtccttcg ctggacccgg
     2101 gagaagcagc caaactatgg gctagccatt gaggtgactc acctccatca gactcggacc
     2161 caccagggcc agcatgtcag gattagccga tcgttacctc aagggagtgg gaattgggcc
     2221 cagctccggc ccctcctggt cacctttggc catgatggcc ggggccatgc cttgacccga
     2281 cgccggaggg ccaagcgtag ccctaagcat cactcacagc gggccaggaa gaagaataag
     2341 aactgccggc gccactcgct ctatgtggac ttcagcgatg tgggctggaa tgactggatt
     2401 gtggccccac caggctacca ggccttctac tgccatgggg actgcccctt tccactggct
     2461 gaccacctca actcaaccaa ccatgccatt gtgcagaccc tggtcaattc tgtcaattcc
     2521 agtatcccca aagcctgttg tgtgcccact gaactgagtg ccatctccat gctgtacctg
     2581 gatgagtatg ataaggtggt actgaaaaat tatcaggaga tggtagtaga gggatgtggg
     2641 tgccgctgcc caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc
     2701 ggtctcgatt ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc
     2761 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2821 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2881 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2941 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3001 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3061 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3121 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     3181 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3241 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3301 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     3361 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     3421 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     3481 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3541 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3601 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     3661 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     3721 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3781 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3841 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     3901 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3961 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4021 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     4081 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4141 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4201 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     4261 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4321 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4381 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4441 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4501 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4561 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4621 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4681 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4741 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4801 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4861 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4921 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4981 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5041 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5101 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5161 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5221 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5281 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5341 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5401 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5461 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5521 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5581 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5641 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5701 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5761 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5821 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5881 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5941 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6001 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6061 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6121 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6181 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6241 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6301 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6361 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6421 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6481 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6541 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6601 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6661 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6721 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6781 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     6841 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     6901 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     6961 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     7021 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     7081 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     7141 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7201 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7261 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     7321 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     7381 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     7441 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     7501 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     7561 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     7621 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     7681 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     7741 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     7801 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     7861 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     7921 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     7981 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     8041 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     8101 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     8161 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     8221 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8281 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     8341 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8401 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8461 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8521 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8581 tttcagaccc acctcccaac cccgagggga cccttgcgcc ttttccaagg cagccctggg
     8641 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     8701 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     8761 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     8821 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     8881 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     8941 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     9001 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     9061 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta
     9121 ccaccggagc ttaccatgac cg
//
