LOCUS       sequence-495624-       10232 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Lac12 for IPTG import.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10232)
  TITLE     Stelling Lab Plasmids 2025
REFERENCE   2  (bases 1 to 10232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P2883
FEATURES             Location/Qualifiers
     source          1..10232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(372..4550)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(4551..4790)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     primer_bind     4892..4914
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4906..4923
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4907..4923
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4930..4949
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4930..4948
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      7439..7688
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7455..7473)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(7851..7869)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7890..7906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7890..7906)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7903..7925)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7914..7930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8304..8321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8475..9063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8555..8574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9242..10033,10034..10102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9865..9884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10103..10207)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaatt ggcgcgcctg tgaagatcac aaggttttgc attgaggata gtatagaagc
      121 aagaatcatt gaattacagg aaaaaaaggc aaatatgatt catgctacaa taaaccaaga
      181 tgaagctgcc attagcagac taacgccagc cgatttacag ttcttattca ataactaata
      241 ttttattctc ttattatata ttattctcgg agtttttaag tgacatcacc cgaaaagaag
      301 ctaagtcttt ctcctaattc atatttaatt attgtacatg gacatatgca tatcatacgt
      361 aatgctcaac cttaagctgc tgcggagctt ccacgagcac cagcacctga agcaactaga
      421 cttatttgcg cttgagttag ttctatactt ggcagtggaa gtttgtcatt atgagttggt
      481 ggaggtaaaa atccaacctt gtttaaaaat gcaatatata taccaacctc ttctggagta
      541 acacctattc cattagacca atcaacacct gtccatgcgg tgtctttctt taaagatgcc
      601 acggcgttcc tatcgtctag ttccggagct ttggtacttt caggtaagtt gtctaagacc
      661 atgtgtagca aaggatacaa cgcattttct tcattcctgt caataacaga cgcctccaat
      721 gatttcttcc atttagaata gctttcgatt tcgacatcgt aaccataatc gtgtaaagta
      781 tacaagtagt ctttgaataa tattcttggg tgacccgtta cttgagcaac ggccaattca
      841 ttttctttgg gaggattcaa agacgtagca acaacaacac gagcaacatg atctactgga
      901 accatattca cggaattttc gatgtctgga atcttaccta attggactga acctttcaaa
      961 aatctcaata agaaatcatc tgtgtttgaa gaaccattgg cagaggcacc tgttacgtaa
     1021 cctggtctga caatacaccc acgtaggccc ctttcacctg cacgtctaat gatgtactca
     1081 gcagcccatt tggactgacc atatccacca gtgagcccgc ttgcagagtt cattaaatcg
     1141 tctgattcta aaatgcctgg cttcccttcg ctaacaagtt tatctgacaa attaaagtag
     1201 tattcagtgt caagagtaga agtggaggaa acaaagtcaa agaactttgg cttgccgacg
     1261 gcggctaagc tcataacatt gatagttgaa ataacatttg gatctctcaa tttggcgtat
     1321 ggataaaccc agtgaactaa cgcaccatta tggataatta tatcaactgt gtttgccaaa
     1381 tccatccact tctcatctga aagaccaaat tggcttttag ataaatcgcc taatacaact
     1441 ttaatatttg aggcaaattt ttcgttccaa gtaccatagg tgatacctgc cttttgtaat
     1501 cttgcaaatg cagcttcttc atccttggcc ctgacgtggg caaacacttt gaaactgtag
     1561 ttctttggag aacgtcctaa caaatctgca aggatgtagg agcccagaaa tcctgtgaca
     1621 ccagtaacaa acacattaat tgttgttttt ccttcggcac tattaggttc aacaaaatat
     1681 tcacgagagg ggtacgaact tggtagcgtc tcaaccaatt tcttggcgtc ttccgcataa
     1741 tttgcagtga cattttcgac gacctcacct tgagatgatc cacccgatga tttaattctg
     1801 tcaatttccg cggcaaaggc ctttatcgtt ggatacttga aaattgtgcc caatggtaaa
     1861 tcaacttgca gctttttctt taaggtaaaa atcattttgg tagccaagat agaatgacca
     1921 cctaaatcga aaaacgaatc atctggtgat acagatgctg gcttggtagg taatatactt
     1981 aaccataagt ctctaacctc gcgctcaaca ttggtaaact gagagtcgtc agtttcagaa
     2041 actgtatttt cagctaccaa atttaattgc ttgggagttg ggaattgaag tttaggctta
     2101 tcaactttac catttggatt caatggtagt ttatccataa ccacaatcaa ggaaggcata
     2161 gcatagctag ccaatctttt ctttaagaaa gtcctgatgt ccttggataa aagatggtaa
     2221 ccgattaagc ccttaactat agggtcagtt tcaacctcct ttggaacatc actttggaac
     2281 ttagacaagt catctggctt gtcaaatctt gggaccataa atgtgatcaa tgttggctca
     2341 ttgtcggcat ttttgcgaac taaagtaatg ttttctctta ccaatggatg ttgggaaatg
     2401 tgcgtatcta tttctcctaa ttcgattctg aacccacgaa ttttaacttg atcatcagcc
     2461 ctaccgcaac attcacagtc accgtttggt agataacgac ctaaatcacc cgttctgtac
     2521 aatctatctc ttggacctaa ccagaattgt ctccaaggtt caccattatc cttatccaaa
     2581 taattccagt gatctttttc aacaaaccag ttgttcacaa atttttcttt attcaattct
     2641 ggtaatcctc tataaccttc ggccaaacca cctgcacgaa cataaatctc acctatttcg
     2701 ccaataccac atatttgagt acggtcgttc ctgttaacaa ctaatagctg aacgttcaac
     2761 atacctttac cagcaggcat gacatctttc aattttttca aaaagtttgg atcgtcattt
     2821 tttgatttaa cttcgaaata agaaactgca cgctgtgttt cagtagtacc gtacatatta
     2881 acaatacggc aattttctgc caaggtttgt aacctcagac aatcacgttt tgttaaaatg
     2941 tcacccacaa agaacgcatg atgtaactta gggaatggtg tagtagcttg ggcagtaagt
     3001 aattgaccca tggcaggtgt taaatgggta actgtgcaac catacttact catccattcc
     3061 gctaaacggc ccggtgtacc aatatcatct tgagtaggga catacaattg ggcacctaaa
     3121 aataatggtg taaacatatc tctttgaatt ggatcatgtg caataccgct cagcattgtg
     3181 aatttatcat tttctgttaa gttgaacctt ttggacatcc aattgaaata ataagccaag
     3241 gaaaaatgtc taccaagaac acccttagga ataccttcgg aaccagatgt gaaagatagg
     3301 gttgggttgg aatctggtcc aactacaaca cctgttctgg tgtctttgta gtgatcgtat
     3361 ggagccaaaa catcctcgcc attgtccaat ttgccacctt caatagtacc attttcttga
     3421 atagcgatgg aattgattct tgaaacaatc tccaattcat cattgatgta atcttctact
     3481 aattgatcca attgtccagc agctctaata acaatcaacc cacgtggttt agcaacacct
     3541 aagtaaatgg tttgtctggc tgggggatat gcagggtcga taactgaaaa ggttgcgcca
     3601 gctttcaaga cacccatcac acataccatc aaatccacac ccctagaaga atagatcatc
     3661 actacatcac ctcttttgat acctgtttta atcaaataat gggcaactat gttagaagtg
     3721 cggttgatgt cgcgataagt gaaagaacgg gacttgtcgg aatttagtgt tggagtctcc
     3781 acaacacagg ttctctctgg gaaggcttca gcattgtcct ggaaaatgtc gtgaatacac
     3841 cccacgaaat cgcaccagcc caagttctta gttggatcag gtaaactatc cttggatgat
     3901 gcggtgatca gagagatttt agttatgcaa ttggatggat cgcttagcgc agcagtcaaa
     3961 tattgagtaa attggtccgc aacaatggtt actctttcat tcgaatacag taagctgtta
     4021 taaattaagt tcaaaacatg cgcattatta ctggtatcca aattcaaagc aaagtccact
     4081 aaatgatgct tgaactcgtc taatttgaaa tcttggtttt ccaaaaaggc caaacggaac
     4141 aactgagggg tcctttccaa gtcttggcaa ctttgaattt tttcagctag ctcgtcaaag
     4201 gaaaaattgg cctcaataga attgagcttg ttcaactcat tgttaattgt agaatacagc
     4261 tcattaaatg accacgttgg ttgaatattg aatcttaaga ttttgttatt cgcaatataa
     4321 agaacaatat catcgtcacc agttactcta tatatcaatg cagcccatac actcaaagcg
     4381 acagcgtatt tgttagaaaa actatcatga ggcacatcga gctgaggtag ctgtaacgaa
     4441 tatgtagctt gtttcgtata aggttcttgt tgtgggcgta aaaagtcatg tggtaacact
     4501 gaaagagttg gattatccaa cttctctatc cagacctttt cgttagtcat tagtgagtaa
     4561 ctctgtgata tgtctctata attagcagtt tttcactgaa attccaggaa aggtaataaa
     4621 ctcagatttt tttttatact attggctgct tgttacttat atatcttgaa cttctcccag
     4681 cgggtcttca aatacatttg ggcgatgttc atgttcatta ggcaggtatt tcgacattga
     4741 gtcacacgcg aaaaaccgcc ggaatttttt atgtaattgc aagtgcaatt ccgctggcaa
     4801 aactattgaa gagttttcct cgctaaaact gtgcgatgcc tctcgatcgg atgtgctgca
     4861 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     4921 agtgaattgt aatacgactc actatagggc gaattggaga aacagaataa tatataaacc
     4981 tgtataatat aaccttgaag actatatttc ttttcgcggc cgcctatagg gcgaattgga
     5041 gctccaccgc ggtggcggcc gctctagaga agcgggtaag ctgccacagc aattaatgca
     5101 caacatttaa cctacattct tccttatcgg atcgtcaaaa cccttaaaaa gatatgcctc
     5161 accctaacat attttccaat taaccctcaa tatttctctg tcacccggcc tctattttcc
     5221 attttcttct ttacccgcca cgcgtttttt tctttcaaat ttttttcttc cttcttcttt
     5281 ttcttccacg tcctcttgca taaataaata aaccgttttg aaaccaaact cgcctctctc
     5341 tctccttttt gaaatatttt tgggtttgtt tgatcctttc cttcccaatc tctcttgttt
     5401 aatatatatt catttatatc acgctctctt tttatcttcc tttttttcct ctctcttgta
     5461 ttcttccttc ccctttctac tcaaaccaag aagaaaaaga aaaggtcaat ctttgttaaa
     5521 gaataggatc ttctactaca tcagctttta gatttttcac gcttactgct tttttcttcc
     5581 caagatcgaa aatttactga attaacaact agtatggcag atcattcgag cagctcatct
     5641 tcgctgcaga agaagccaat taatactatc gagcataaag acactttggg caatgatcgg
     5701 gatcacaagg aagccttgaa cagtgataat gataatactt ctggattgaa aatcaatggt
     5761 gtccccatcg aggacgctag agaggaagtg ctcttaccag gtttcttgtc gaagcaatat
     5821 tacaaattgt acggtttatg ttttgtaaca tatctgtgtg ctactatgca aggttatgat
     5881 ggggctttaa tgggttctat ctataccgaa aatgaatatt tgaaatacta ccatttggat
     5941 attaactcat cctctggtac tggtctagtg ttctctattt tcaacgttgg tcaaatttgc
     6001 ggtgcattct ttgttcctct tatggattgg aaaggtagaa aacctgctat tttaattggg
     6061 tgtctgggtg ttgttattgg tgctattatt tcgtctttaa caacaacaaa gagtgcatta
     6121 attggtggta gatggttctt ggcctttttc gctacaatcg ctaattcagc agctccaaca
     6181 tactgtgcag aagtggctcc agctcactta agaggtaagg ttgcaggtct ttataacacc
     6241 ctttggtctg tcggttccat tgttgctgcc tttaccactt acggtactaa caaaaacttc
     6301 cctaactcct ccaaggcttt taagattcca ttatacttac aaatgatgtt cccaggtctt
     6361 gtgtgtatat ttggttggtt aatcccagaa tctccaagat ggttggttgg tgttggccgt
     6421 gaggaagaag ctcgtgaatt tattatcaaa taccacttaa atggcgatag aactcatcca
     6481 ttattggata tggagatggc agaaataata gaatctttcc atggtacaga tttatcaaac
     6541 ccactagaaa tgttagatgt aaggagctta ttcagaacga gatcggatag gtacagagca
     6601 atgttggtta tacttatggc ttggttcggt caattttccg gtaacaatgt gtgttcgtac
     6661 tatttgccta ccatgttgag aaatgttggt atgaagagtg tctcattgaa tgtgttaatg
     6721 aatggtgttt attccatcgt ctcttggatt tcttcaattt gcggtgcatt ctttattgat
     6781 aagattggta gaagggaagg tttccttggt tctatctcag gtgctgcatt agcattgaca
     6841 ggtctatcta tctgtactgc tcgttatgag aagactaaga agaagagtgc ttccaatggt
     6901 gcattggtgt tcatttatct ctttggtgct atcttttctt ttgctttcac tccaatgcaa
     6961 tccatgtact caacagaagt gtctacaaac ttgacgagat ccaaggccca actcctcaac
     7021 atgttggttt ctggtgttgc ccaatttgtt aatcaatttg ctactccaaa ggcaatgaag
     7081 aatatcaaat attggttcta tgtgttctac gttttcttcg atattttcga atttattgtt
     7141 atctacttct tcttcgttga aactaagggt agatccttag aagaattaga agttgtcttt
     7201 gaagctccaa acccaagaaa ggcatccgtt gatcaagcat tcttggctca agtcagggca
     7261 actttggtcc aacgaaatga cgttagagtt gcaaatgctc aaaatttgaa agagcaagag
     7321 cctctaaaga gcgatgctga tcatgtcgaa aagctatcag aggcagaatc tgttaagctt
     7381 ggtggatccg aattctaaga tatctagctc gagacaggcc ccttttcctt tgtctatatc
     7441 atgtaattag ttatgtcacg cttacattca cgccctcctc ccacatccgc tctaaccgaa
     7501 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttta atagttatgt
     7561 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac aaacgcgtgt
     7621 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt
     7681 taatttgcaa ggttcgcagt ttacactctc atcgtcgctc tcatcatcgc ttccgttgtt
     7741 gttttcctta gtagcgtctg cttggtaccc agcttcggcc gtatttacag ataacgatgt
     7801 cgttattata tatatatata tatattgtca cttgtaccca gcttttgttc cctttagtga
     7861 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7921 ccgctcacaa cagctgagaa gcggtcagca agaagaaaat cgaataatca gtttcaatga
     7981 atatagacgt aacaaaggca caaaaaagaa aacaagtaga tatgtcatac atatatatat
     8041 atttcaggca gcaagtgacc atcaaccagg aagtgtgaaa tagcaaagaa ctgtttactt
     8101 cttcagactt agaaagctct tcccatagga gaaagcaact gctttccttt gtagtaattg
     8161 attgtctttt cagcccttga cgcaacaccg tccgctgtga aatcgaacaa tttgtaaatt
     8221 tcaggcgcgc catcctgcag gcattaatga atcggccaac gcgcggggag aggcggtttg
     8281 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8341 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8401 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8461 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8521 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8581 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8641 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8701 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8761 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8821 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8881 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8941 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9001 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9061 caagaagatc ctttgatctt ttgtttaaac ctacggggtc tgacgctcag tggaacgaaa
     9121 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9181 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9241 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9301 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9361 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9421 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9481 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9541 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9601 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9661 cggttagctc cttcggtcct ccgatggttg tcagaagtaa gttggccgca gtgttatcac
     9721 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9781 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9841 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9901 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9961 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10021 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10081 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10141 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10201 ttccgcgcac atttccccga aaagtgccac ct
//
