LOCUS       sequence-495639-        5077 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5077)
  TITLE     PKC plasmids
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC19 PKC alpha
FEATURES             Location/Qualifiers
     source          1..5077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2478..2494)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2478..2494)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2491..2513)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2502..2518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2526..2532,2533..2550,2551..2556))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2571..2592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2709..2726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2880..3468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2960..2979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3639..4430,4431..4499))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4262..4281
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4500..4604)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4672..4690
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4728..4750)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4850..4869
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(5063..5077,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(5077,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttcgagctcg gtacccgggg atcctctaga gtcaattccg
       61 ggccgcgagt aggaagcgcg agcgccgggc gccgggctgt cagtgagcgt ggggccagcc
      121 agcgagccag agagccggag agagccagag agagcggctc ggctcccagc tccgagtagc
      181 gcagcgcccg cccggctctc ccgggccacc gccgccacca ccgcacctca tcagcaccgc
      241 cacctcggcc gccgcccccg cccaccccgg ccctccccgg ctgctgctcc ccggcggagg
      301 caagaggtgg ttggggggaa ccatggctga cgtttacccg gccaacgact ccacggcgtc
      361 tcaggacgtg gccaaccgct tcgcccgcaa aggggcgctg aggcagaaga acgtgcatga
      421 ggtgaaagac cacaaattca tcgcccgctt cttcaagcaa cccaccttct gcagccactg
      481 caccgacttc atctgggggt ttgggaaaca aggcttccag tgccaagttt gctgttttgt
      541 ggttcataag aggtgccatg agttcgttac gttctcttgt ccgggtgcgg ataagggacc
      601 tgacactgac gaccccagga gcaagcacaa gttcaaaatc cacacatacg gaagccctac
      661 cttctgtgat cactgtgggt ccctgctcta tggacttatc caccaaggga tgaaatgtga
      721 cacctgcgac atgaatgttc acaagcagtg tgtgatcaat gtccctagcc tctgcggaat
      781 ggatcacaca gagaagaggg ggcggattta tctgaaggct gaggtcactg atgaaaagct
      841 ccacgtcacg gtacgagatg caaaaaatct aatccctatg gatccaaatg ggctttcgga
      901 tccttatgtg aagctgaaac ttatccctga ccccaagaat gagagcaaac agaaaaccaa
      961 aaccatccgc tccacactga atcctcagtg gaatgagtcc ttcacgttca aattaaaacc
     1021 ttcagacaaa gaccggcgac tgtctgtaga aatctgggac tgggatcgga cgactcggaa
     1081 tgacttcatg ggatcccttt cctttggtgt ctcagagcta atgaagatgc cggccagtgg
     1141 atggtataaa ctgctcaacc aagaagaggg cgaatattac aatgtgccca ttccagaagg
     1201 agatgaagaa ggcaacatgg aactcaggca gaagtttgag aaagccaagc taggccctgc
     1261 tggtaacaaa gtcatcagcc cttcagaaga cagaaagcaa ccatccaaca acctggacag
     1321 agtgaaactc acagacttca acttcctcat ggtgctgggg aaggggagtt ttgggaaggt
     1381 gatgcttgct gacaggaagg gaacggagga actgtacgcc atcaagatcc tgaagaagga
     1441 cgtggtgatc caggacgacg acgtggagtg caccatggtg gagaagcgcg tgctggccct
     1501 gctggacaag ccgccatttc tgacacagct gcactcctgc ttccagacag tggaccggct
     1561 gtacttcgtc atggaatacg tcaacggcgg ggacctcatg taccacattc agcaagtcgg
     1621 gaaatttaag gagccacaag cagtattcta cgcagccgag atctccatcg gactgttctt
     1681 ccttcataaa agagggatca tttacaggga tctgaagctg gacaatgtca tgctggactc
     1741 agaagggcac atcaaaatcg ccgacttcgg gatgtgcaag gaacacatga tggatggagt
     1801 cacgaccagg accttctgtg ggactccgga ctacattgcc ccagagataa tcgcttacca
     1861 gccgtacggg aagtctgtag attggtgggc gtacggtgtg ctgctgtacg agatgctagc
     1921 cgggcagcct ccgtttgatg gtgaagatga agatgaactg tttcagtcta taatggagca
     1981 caacgtgtcc taccccaaat ccttgtccaa ggaagccgtc tccatctgca aaggacttat
     2041 gaccaaacac cctgccaagc ggctgggctg cgggcccgag ggagagaggg atgtcagaga
     2101 gcatgccttc ttcaggagga tcgactggga gaaactggag aacagggaga tccaaccacc
     2161 attcaagccc aaagtgtgtg gcaaaggagc agaaaacttt gacaagttct tcacgcgagg
     2221 acagcctgtc ttaacaccac cagatcagct ggtcattgct aacatagacc aatctgattt
     2281 tgaagggttc tcgtatgtca acccccagtt tgtgcaccca atcttgcaaa gtgcagtatg
     2341 aaactcaaaa atgaaagatc aaatgcctcc ccagccccca gcatccccag cagtgggaag
     2401 tgatccttaa ccataaaaat ttaaggctaa aaaaaaccgg aattgacctg caggcatgca
     2461 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2521 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2581 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2641 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2701 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2761 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2821 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2881 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2941 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3001 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3061 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3121 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3181 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3241 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3301 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3361 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3421 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3481 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3541 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3601 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3661 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3721 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3781 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3841 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3901 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3961 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4021 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4081 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4141 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4201 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4261 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4321 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4381 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4441 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4501 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4561 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4621 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     4681 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     4741 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     4801 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     4861 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     4921 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4981 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     5041 agttgggtaa cgccagggtt ttcccagtca cgacgtt
//
