LOCUS       sequence-49564-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Perez-Pinera P, Ousterout DG, Brown MT, Gersbach CA
  TITLE     Gene targeting to the ROSA26 locus directed by engineered zinc 
            finger nucleases.
  JOURNAL   Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14.
  PUBMED    22169954
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-04-1"; volume: "40(8)"; pages: "3741-52. 
            Epub 2011 Dec 14"
COMMENT     Sequence Label: pCMV-RosaL6 ELD mutations
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        186..204
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     186..203
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     217..236
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             283..309
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             337..357
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttgacctcc ntagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt
       61 ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt ctataggccc
      121 acccccttgg cttcgttaga acgcggctac aattaataca taaccttatg tatcatacac
      181 atacgattta ggtgacacta tagaataaca tccactttgc ctttctctcc acaggtgtcc
      241 actcccaggt ccaactgcac ctcggttcta tcgattgaca tgtacccata cgatgtccca
      301 gactacgcga attcccctgg gatcccaggc atggggccca aaaagaaacg caaagttggg
      361 cgcctcgagc ccggggaaaa accctataaa tgccccgagt gtggtaagtc attctctcaa
      421 agcggggatt taagaagaca ccagagaacc cacaccgggg aaaagcccta caagtgtcct
      481 gagtgcggaa agtctttctc cactagcggt tcattagtaa gacaccagag gacacacacc
      541 ggggagaaac cttataaatg cccagaatgc gggaaatcgt tcagtcaaag agcacattta
      601 gaaagacatc aacggaccca caccggggaa aaaccataca agtgccccga gtgtggcaag
      661 agctttagta cccacctcga cctgattaga caccagcgca cccacaccgg ggaaaagcca
      721 tataagtgcc ctgagtgtgg aaagagcttc agtaggaagg ataaccttaa aaaccaccaa
      781 agaacccaca ccggggaaaa gccctataag tgcccagagt gcgggaaatc attctc
//
