LOCUS       sequence-495715-       23030 bp    DNA     circular SYN 18-DEC-2025
DEFINITION  Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23030)
  AUTHORS   Chen Z, Debernardi JM, Dubcovsky J, Gallavotti A
  TITLE     The combination of morphogenic regulators BABY BOOM and GRF-GIF 
            improves maize transformation efficiency
  JOURNAL   bioRxiv 2022.09.02.506370
REFERENCE   2  (bases 1 to 23030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.09.02.506370"
COMMENT     Sequence Label: pBUE411-GGB
FEATURES             Location/Qualifiers
     source          1..23030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(60..312)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(346..435)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    464..488
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2397..2406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2403..2427)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     4630..4652
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4644..4661
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4645..4661
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(4712..4964)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4978..5002
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5043..5052
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             6189..6200
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(7154..7178)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8307..10299
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             10318..10383
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             10390..10410
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10435..14535
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             10435..14535
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             14448..14456
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             14536..14583
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(15240..15256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15240..15256)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15253..15275)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15264..15280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15288..15294,15295..15312,15313..15318))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15333..15354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        15545..15890
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     15833..15853
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             15941..16492
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    16499..16673
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    16751..16775
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             17200..17994
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      18081..18669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18570..18589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     18823..18840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    18855..18995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(18921..18940)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     19081..19103
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      19339..19533
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(19599..20672)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(21101..21730)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc tgcaggcgcg ctaattcccg
       61 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
      121 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      181 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
      241 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      301 gtttgaacga tcggggaaat tcgagctcta agcgctgcac tgagcagcgt aatctggaac
      361 gtcatatgga taggatcctg catagtccgg gacgtcatag ggatagcccg catagtcagg
      421 aacatcgtat gggtaaaaga tgttaattaa cccgctgtta tcaaccactt tgtacaagaa
      481 agctgaacgc tagcttcctt cctcctcggt gcccttgagg tactccccgt cgccgctgtg
      541 cgcgccagag ttggcgccgc gagcgtcggc cgacagcgag gtcgacccct ccttggcgcc
      601 gctgccgccg cggccatagt ccgagaagac gctggcgttc atcatgctgt tgccagtggc
      661 gccgccgccg ccgcccatgc tggcctcacc gtgcaggagg ttgaacccgg tggtcgcgcc
      721 ggaggacatc cccagctggc cgtgcgcggc cgcctggtgc tgctgctgct gctgctgctg
      781 ctggagcggc gacatggcct gctgcgcgta catcatcgac gaccgcgccg ccatcagcga
      841 ctgcggcgac atcatctgcg ccgactgctg ctgcatgtac cgcggcccgg actgcatcat
      901 caggttggac gggtactgag acagcgatgc cgtctgcgga ggctggctgt cggcgatggc
      961 ggcgaggtac atgaggttct gctggagctt agcttggttc cgtgcgcact cctccacctt
     1021 gccgttgttc tggttgtcga ggatggccag gatcagctgc ttgttctcat ccaggtactg
     1081 ctgaatgaga tcagtggtgg cagtggtaga ggaagcgtag ccccccatca tgctctggtt
     1141 catctgcatc aggtgttgct gctgcatggc agcggccgcg tacatttcgc cggcgaacag
     1201 catgccgttg ggcgactggg agctggcggc ggagaagtcg gaggacgtca tgggtatcga
     1261 gatcgacagc tgggtggccg agaaggaggc gacgttcgag ttgtcgtcgg tcaggtccga
     1321 ccacgagtcc ctcgtcttcg gccactcgtc gaagaacggc cgcagcgtct ggttctcctg
     1381 cttcgcggag tcgccgctgc cgaagtcgcc gcaccccggg aaggagagcg gctgctgctg
     1441 ctgctgtccc tcggccttga cgccgctgtt gttgttgctg ctgttgttct gacccaggtc
     1501 actcgttgct ccaagctgag gagagtagct tgagagcggg aatgtagtcg tttgagaaga
     1561 tggcggcagg cgccatgggt tctccacgga ggtgttcatg gcttctgtca tgagctgact
     1621 ctgctcgtct gccaaagatc ttattccata tgcggtatac ctgaaatctt tgcatgttcc
     1681 ggcgccgccg agagccgcgt agggagaggc ggcattgttg cccatgtgct gctgaggcgg
     1741 ccccagcgcg gaggagaacg tgccgggcat ggacatgccg ttgttccccc cgcctccacc
     1801 accgttggtg ccgccgatgg cggggtagag ggagtggttg tggaagccgg tggcgagggg
     1861 cggcacgacg gaggcggccg gcggctggga gtgcgagacg agctgcgttt ccacaggctt
     1921 tcttgaacgg ttgcggccgc ggtgcatgtg gcgctcgcaa tacttggagt cggaggcggc
     1981 ctccttggcg caccgccact tcttgccgtc cgtgcgccgg caccggcccg gctccggatc
     2041 caccttcttg cccaggtacg atccgtaccc gatggcgagg gggttgtggt agaagcgggc
     2101 ggcgagggac tcgatgccgc ggcggatggg gagcacgaga tccggcggga cggacacgcc
     2161 ggccaccagg tacttgtata tgagcgcctg gtgctccagc tcctcgtact gcgccgccgt
     2221 gaagggcacc ggcctcgcca tccacctccc gtccatccgc gcctcctccc ccatgccgcc
     2281 attgccgccg gcggagaagg gggaggagcg gcagaagggg aggagggagg cggtggcggt
     2341 ggcggccggg gaggagcggc ggtcgcctgc cggggaaaga gaggcatacg gcatcgccat
     2401 ggtggcagct tttttgtaca aacttgttga taactctagt ctgcagaagt aacaccaaac
     2461 aacagggtga gcatcgacaa aagaaacagt accaagcaaa taaatagcgt atgaaggcag
     2521 ggctaaaaaa atccacatat agctgctgca tatgccatca tccaagtata tcaagatcaa
     2581 aataattata aaacatactt gtttattata atagataggt actcaaggtt agagcatatg
     2641 aatagatgct gcatatgcca tcatgtatat gcatcagtaa aacccacatc aacatgtata
     2701 cctatcctag atcgatattt ccatccatct taaactcgta actatgaaga tgtatgacac
     2761 acacatacag ttccaaaatt aataaataca ccaggtagtt tgaaacagta ttctactccg
     2821 atctagaacg aatgaacgac cgcccaacca caccacatca tcacaaccaa gcgaacaaaa
     2881 agcatctctg tatatgcatc agtaaaaccc gcatcaacat gtatacctat cctagatcga
     2941 tatttccatc catcatcttc aattcgtaac tatgaatatg tatggcacac acatacagat
     3001 ccaaaattaa taaatccacc aggtagtttg aaacagaatt ctactccgat ctagaacgac
     3061 cgcccaacca gaccacatca tcacaaccaa gacaaaaaaa agcatgaaaa gatgacccga
     3121 caaacaagtg cacggcatat attgaaataa aggaaaaggg caaaccaaac cctatgcaac
     3181 gaaacaaaaa aaatcatgaa atcgatcccg tctgcggaac ggctagagcc atcccaggat
     3241 tccccaaaga gaaacactgg caagttagca atcagaacgt gtctgacgta caggtcgcat
     3301 ccgtgtacga acgctagcag cacggatcta acacaaacac ggatctaaca caaacatgaa
     3361 cagaagtaga actaccgggc cctaaccatg gaccggaacg ccgatctaga gaaggtagag
     3421 aggggggggg gggaggacga gcggcgtacc ttgaagcgga ggtgccgacg ggtggatttg
     3481 ggggagatct ggttgtgtgt gtgtgcgctc cgaacaacac gaggttgggg aaagagggtg
     3541 tggagggggt gtctatttat tacggcgggc gaggaaggga aagcgaagga gcggtgggaa
     3601 aggaatcccc cgtagctgcc ggtgccgtga gaggaggagg aggccgcctg ccgtgccggc
     3661 tcacgtctgc cgctccgcca cgcaatttct ggatgccgac agcggagcaa gtccaacggt
     3721 ggagcggaac tctcgagagg ggtccagagg cagcgacaga gatgccgtgc cgtctgcttc
     3781 gcttggcccg acgcgacgct gctggttcgc tggttggtgt ccgttagact cgtcgacggc
     3841 gtttaacagg ctggcattat ctactcgaaa caagaaaaat gtttccttag tttttttaat
     3901 ttcttaaagg gtatttgttt aatttttagt cactttattt tattctattt tatatctaaa
     3961 ttattaaata aaaaaactaa aatagagttt tagttttctt aatttagagg ctaaaataga
     4021 ataaaataga tgtactaaaa aaattagtct ataaaaacca ttaaccctaa accctaaatg
     4081 gatgtactaa taaaatggat gaagtattat ataggtgaag ctatttgcaa aaaaaaagga
     4141 gaacacatgc acactaaaaa gataaaactg tagagtcctg ttgtcaaaat actcaattgt
     4201 cctttagacc atgtctaact gttcatttat atgattctct aaaacactga tattattgta
     4261 gtactataga ttatattatt cgtagagtaa agtttaaata tatgtataaa gatagataaa
     4321 ctgcacttca aacaagtgtg acaaaaaaaa tatgtggtaa ttttttataa cttagacatg
     4381 caatgctcat tatctctaga gaggggcacg accgggtcac gctgcacgcg cctgcagatc
     4441 cccgggtacc gagctcaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc
     4501 tctagcattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     4561 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     4621 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagcta tggtgagacc
     4681 tcggtctcgc tccactagtt cggtacccgg ggatctagta acatagatga caccgcgcgc
     4741 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
     4801 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
     4861 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
     4921 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcttaatt aaatcaaacc
     4981 actttgtaca agaaagctgg gcccatgagg ccagtgtcgt tccagacact gaagagctgc
     5041 gcgccgccat ggccgacgtc ggcggcaatg ttgtcgttgc tgcttgctgc tgccgccgcg
     5101 gctgcagaga cgacggtccc ccatgcagac atccttccgc caccattgtt ctccgcgttc
     5161 accaggtagc tctcgtaccc catctgagca gcctgcttgg cttcgtcgta ggcccgtgca
     5221 tgcatctgct cgtggctcac cattgccgat gtagtggttg ctccggcagc gctcatcggc
     5281 atcatgtagc caccgccgct gccaacggcg ctggcgccgt tgctatcgcc gaccccgccg
     5341 ttgtagacga cggagttgga gccggtgctg tgctcgagcg acgcactgtc gatgctagcc
     5401 aggccgtgca tcgcagctgc ggcggcggcc tgctgccctg ccgagaaaaa gtcgtgcgcg
     5461 ccggccgcgc ccaggttcaa gtggtggagg tcctgcaggc tgtgcgcggc cgcgatcacc
     5521 gcgtggtcct gctcctgctt gcaccacccg ccgccgcgca ttggctgctg cgcgtacggg
     5581 tggtacaggc ctgtggtggc ggcgcccggc tggaacgcga tggtcggcca ggcggcgccg
     5641 tggtagtgcg cgccgtacgc cgccgctagg gctccgccgt cgccgagctg cgaggcgatg
     5701 cggccgacgt cgtagctcac cacgccggcg tggtggtgct gcgcggacgc tgcggcctcg
     5761 gcctccttga gacgcttggc ggcgctgccg atggggaggg cgctgctgtc caggatgctc
     5821 ttcacgtcgt agcggctcat gtcgaagttg gtgacggcgt tgaggccgcg gaacttgatc
     5881 gccgcgatgt cgtacgcctc cgctgcctcc tcctgggtgc tgaaggtgcc caagtaaaga
     5941 tccttgttcc ctgcaactcg tccaatccgt gcttgccatc ttccatgttg gtgatgccta
     6001 gtcactcccc tgtaaatgga tgcacctctg gagaaaccac tgctcttcct tctcagagac
     6061 gctacaaact cctgccttgt catgtgcttc atgtcctcga gctccttttc gtagttactc
     6121 actggaaaat ttgttgttgt tgtggcaccc cagtacttca gagcagcaag atcataagcc
     6181 ctagcagctt tctcctcttt atcatagcca cctaaataga cttgacgacc cttacgagtt
     6241 tgtccttccc ttctgcaact gttatcccaa agatgtgcct catatctccc agtccatcta
     6301 tgccttgtca cgccacggta aatcgacgtg cgctgcccga acgtgtccac cgtcttcctt
     6361 gccgtgttgt cagccgacgc gccgccgctg ccacccgtgt ccgtgctcac ggctactaga
     6421 gcaccggcaa caccgctgcc accgctatcc tccttcggcg ccgtgacgac gacggcacca
     6481 ccctgtgctg acgtcgatac actctcgggc gcccgtgcgc gctctccagc gagaagtggc
     6541 atgccagcag cgccttgggt cgtccccgcc atgttcatgg acaaagagag cccctgcgcg
     6601 ccctcagccg ccgccgccct cggctgcatg ggcgccggtt ggctccgcag ccagttcttg
     6661 atcatggaca gcccgatgcc gccgccgttg gcactggcag cgccagcgac accgttagcg
     6721 gcggccgcgc tgagcatgga accgccgtcg tggacaccgg ccgaggaggc caccatgacg
     6781 gagtccgcga agtggagcgc ggagctggtg ggctggtaca gctggtgatg gtagccggtg
     6841 ctagcagctg agctcgcgta gcaaactgtg ctgctagtgc tgggtatcaa gttgcagttg
     6901 gacttgtgat gctgctcgga gaaggagatg ccgccgagga agtcctccag cttcggctcc
     6961 gcgacgagcg ccgaaagctc tgatcccctc atgctccaat cttgggggat gttgaagcag
     7021 acatcgccgg agacatggtc ggcggtggcg gccgagatga gcgtggagtc cgtcgtctgg
     7081 gagggcggca gctcctgcgg ggagagggag aaagcgagcc agttgttcac agtggccatt
     7141 ggccttgacg gcccttctgc ttttttgtac aaacttgttt gatggatccg ttccttgagg
     7201 agggaggtgc ctgccgctct gctagctcgt cgggctgttt ggaatgcggc ggtcggtaga
     7261 tggcaagtgc tttgctagag ctagctggtg atgagtggtg aggagagttg cagtgggatg
     7321 tcgctcctta aataggcctg tgtacgtgtg atcggtggaa aatggaaata atggtggatg
     7381 gatggatgca cgcgtatcgg cgtatggatg caagcttgca tggtggatgt tccgtcgtcg
     7441 tcgtcgtctt cttccttgga tggcatcgat ccgtgtccaa gttattaaga gccgagcagg
     7501 aggtggttgg gtgttggagg gaggcatgta cagtatatat gcagagttaa ttcatcatca
     7561 gcgcagtagt agtatactaa ctggctagac tagatactcc tatgtagctg gcgccgccta
     7621 acgcgacttt cgagcagggt gatggcggca gcctgagccg gccggcagac gtcgtatacg
     7681 tacgtggctc atctgcggcg gcagtcgtcg gatgtctctc tttctctcta tcagtgtgtc
     7741 agtgtggagg tagtttgcgg ttggatctga gtgcttatcg cgcaattaac acgtacatcc
     7801 tccggccacc tacattaaga gacaccacat ccatcatcca tggacgatgc ttccagtagt
     7861 cttcgtcctc gtcccttcct cccactttcc tccttagagc gagtttaatt aataataata
     7921 ataatagagc ctatatatta ttagctctaa ctccttacca tatcatctaa agccaacaat
     7981 aaacacattc atataataaa gttggctctt agataaatct actctcttcc tgtttcatta
     8041 ttttgtcttg aagctctttt agcaccgggc cgtccgccgt tgcatgagaa ccactctctc
     8101 taatttttat atgtctcttc cacaataaaa aatgtcatgt aagcagactt aaaacctatt
     8161 atcatacata ctcttgcact gttacacagg ctacaaccgc aagacagaga ttgatggggg
     8221 acgcgtacag cgcaggagct aggttacatg cacattcaaa aaattcaagt agcagcagta
     8281 gcagcgtggc gcgccagctt gcatgcctgc agtgcagcgt gacccggtcg tgcccctctc
     8341 tagagataat gagcattgca tgtctaagtt ataaaaaatt accacatatt ttttttgtca
     8401 cacttgtttg aagtgcagtt tatctatctt tatacatata tttaaacttt actctacgaa
     8461 taatataatc tatagtacta caataatatc agtgttttag agaatcatat aaatgaacag
     8521 ttagacatgg tctaaaggac aattgagtat tttgacaaca ggactctaca gttttatctt
     8581 tttagtgtgc atgtgttctc cttttttttt gcaaatagct tcacctatat aatacttcat
     8641 ccattttatt agtacatcca tttagggttt agggttaatg gtttttatag actaattttt
     8701 ttagtacatc tattttattc tattttagcc tctaaattaa gaaaactaaa actctatttt
     8761 agttttttta tttaataatt tagatataaa atagaataaa ataaagtgac taaaaattaa
     8821 acaaataccc tttaagaaat taaaaaaact aaggaaacat ttttcttgtt tcgagtagat
     8881 aatgccagcc tgttaaacgc cgtcgacgag tctaacggac accaaccagc gaaccagcag
     8941 cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc tctggacccc
     9001 tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag aaattgcgtg
     9061 gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg
     9121 cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat
     9181 aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac
     9241 acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc
     9301 gtcctccccc cccccccctc tctaccttct ctagatcggc gttccggtcc atggttaggg
     9361 cccggtagtt ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc
     9421 tgctagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt gctaacttgc
     9481 cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac gggatcgatt
     9541 tcatgatttt ttttgtttcg ttgcataggg tttggtttgc ccttttcctt tatttcaata
     9601 tatgccgtgc acttgtttgt cgggtcatct tttcatgctt ttttttgtct tggttgtgat
     9661 gatgtggtct ggttgggcgg tcgttctaga tcggagtaga attctgtttc aaactacctg
     9721 gtggatttat taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg
     9781 aagatgatgg atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg
     9841 atgcatatac agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg
     9901 tcgttcattc gttctagatc ggagtagaat actgtttcaa actacctggt gtatttatta
     9961 attttggaac tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga
    10021 aatatcgatc taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat
    10081 ggcatatgca gcatctattc atatgctcta accttgagta cctatctatt ataataaaca
    10141 agtatgtttt ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta
    10201 tatgtggatt tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt
    10261 tgtcgatgct caccctgttg tttggtgtta cttctgcagc cctaggccta ctagatggat
    10321 tacaaggacc acgacgggga ttacaaggac cacgacattg attacaagga tgatgatgac
    10381 aagatggctc cgaagaagaa gaggaaggtt ggcatccacg gggtgccagc tgctgacaag
    10441 aagtactcga tcggcctcga tattgggact aactctgttg gctgggccgt gatcaccgac
    10501 gagtacaagg tgccctcaaa gaagttcaag gtcctgggca acaccgatcg gcattccatc
    10561 aagaagaatc tcattggcgc tctcctgttc gacagcggcg agacggctga ggctacgcgg
    10621 ctcaagcgca ccgcccgcag gcggtacacg cgcaggaaga atcgcatctg ctacctgcag
    10681 gagattttct ccaacgagat ggcgaaggtt gacgattctt tcttccacag gctggaggag
    10741 tcattcctcg tggaggagga taagaagcac gagcggcatc caatcttcgg caacattgtc
    10801 gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg
    10861 gactccacag ataaggcgga cctccgcctg atctacctcg ctctggccca catgattaag
    10921 ttcaggggcc atttcctgat cgagggggat ctcaacccgg acaatagcga tgttgacaag
    10981 ctgttcatcc agctcgtgca gacgtacaac cagctcttcg aggagaaccc cattaatgcg
    11041 tcaggcgtcg acgcgaaggc tatcctgtcc gctaggctct cgaagtctcg gcgcctcgag
    11101 aacctgatcg cccagctgcc gggcgagaag aagaacggcc tgttcgggaa tctcattgcg
    11161 ctcagcctgg ggctcacgcc caacttcaag tcgaatttcg atctcgctga ggacgccaag
    11221 ctgcagctct ccaaggacac atacgacgat gacctggata acctcctggc ccagatcggc
    11281 gatcagtacg cggacctgtt cctcgctgcc aagaatctgt cggacgccat cctcctgtct
    11341 gatattctca gggtgaacac cgagattacg aaggctccgc tctcagcctc catgatcaag
    11401 cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtcag gcagcagctc
    11461 cccgagaagt acaaggagat cttcttcgat cagtcgaaga acggctacgc tgggtacatt
    11521 gacggcgggg cctctcagga ggagttctac aagttcatca agccgattct ggagaagatg
    11581 gacggcacgg aggagctgct ggtgaagctc aatcgcgagg acctcctgag gaagcagcgg
    11641 acattcgata acggcagcat cccacaccag attcatctcg gggagctgca cgctatcctg
    11701 aggaggcagg aggacttcta ccctttcctc aaggataacc gcgagaagat cgagaagatt
    11761 ctgactttca ggatcccgta ctacgtcggc ccactcgcta ggggcaactc ccgcttcgct
    11821 tggatgaccc gcaagtcaga ggagacgatc acgccgtgga acttcgagga ggtggtcgac
    11881 aagggcgcta gcgctcagtc gttcatcgag aggatgacga atttcgacaa gaacctgcca
    11941 aatgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcacagt ctacaacgag
    12001 ctgactaagg tgaagtatgt gaccgagggc atgaggaagc cggctttcct gtctggggag
    12061 cagaagaagg ccatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcag
    12121 ctcaaggagg actacttcaa gaagattgag tgcttcgatt cggtcgagat ctctggcgtt
    12181 gaggaccgct tcaacgcctc cctggggacc taccacgatc tcctgaagat cattaaggat
    12241 aaggacttcc tggacaacga ggagaatgag gatatcctcg aggacattgt gctgacactc
    12301 actctgttcg aggaccggga gatgatcgag gagcgcctga agacttacgc ccatctcttc
    12361 gatgacaagg tcatgaagca gctcaagagg aggaggtaca ccggctgggg gaggctgagc
    12421 aggaagctca tcaacggcat tcgggacaag cagtccggga agacgatcct cgacttcctg
    12481 aagagcgatg gcttcgcgaa ccgcaatttc atgcagctga ttcacgatga cagcctcaca
    12541 ttcaaggagg atatccagaa ggctcaggtg agcggccagg gggactcgct gcacgagcat
    12601 atcgcgaacc tcgctggctc gccagctatc aagaagggga ttctgcagac cgtgaaggtt
    12661 gtggacgagc tggtgaaggt catgggcagg cacaagcctg agaacatcgt cattgagatg
    12721 gcccgggaga atcagaccac gcagaagggc cagaagaact cacgcgagag gatgaagagg
    12781 atcgaggagg gcattaagga gctggggtcc cagatcctca aggagcaccc ggtggagaac
    12841 acgcagctgc agaatgagaa gctctacctg tactacctcc agaatggccg cgatatgtat
    12901 gtggaccagg agctggatat taacaggctc agcgattacg acgtcgatca tatcgttcca
    12961 cagtcattcc tgaaggatga ctccattgac aacaaggtcc tcaccaggtc ggacaagaac
    13021 cggggcaagt ctgataatgt tccttcagag gaggtcgtta agaagatgaa gaactactgg
    13081 cgccagctcc tgaatgccaa gctgatcacg cagcggaagt tcgataacct cacaaaggct
    13141 gagaggggcg ggctctctga gctggacaag gcgggcttca tcaagaggca gctggtcgag
    13201 acacggcaga tcactaagca cgttgcgcag attctcgact cacggatgaa cactaagtac
    13261 gatgagaatg acaagctgat ccgcgaggtg aaggtcatca ccctgaagtc aaagctcgtc
    13321 tccgacttca ggaaggattt ccagttctac aaggttcggg agatcaacaa ttaccaccat
    13381 gcccatgacg cgtacctgaa cgcggtggtc ggcacagctc tgatcaagaa gtacccaaag
    13441 ctcgagagcg agttcgtgta cggggactac aaggtttacg atgtgaggaa gatgatcgcc
    13501 aagtcggagc aggagattgg caaggctacc gccaagtact tcttctactc taacattatg
    13561 aatttcttca agacagagat cactctggcc aatggcgaga tccggaagcg ccccctcatc
    13621 gagacgaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc
    13681 aggaaggttc tctccatgcc acaagtgaat atcgtcaaga agacagaggt ccagactggc
    13741 gggttctcta aggagtcaat tctgcctaag cggaacagcg acaagctcat cgcccgcaag
    13801 aaggactggg atccgaagaa gtacggcggg ttcgacagcc ccactgtggc ctactcggtc
    13861 ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctg
    13921 ctggggatca cgattatgga gcgctccagc ttcgagaaga acccgatcga tttcctggag
    13981 gcgaagggct acaaggaggt gaagaaggac ctgatcatta agctccccaa gtactcactc
    14041 ttcgagctgg agaacggcag gaagcggatg ctggcttccg ctggcgagct gcagaagggg
    14101 aacgagctgg ctctgccgtc caagtatgtg aacttcctct acctggcctc ccactacgag
    14161 aagctcaagg gcagccccga ggacaacgag cagaagcagc tgttcgtcga gcagcacaag
    14221 cattacctcg acgagatcat tgagcagatt tccgagttct ccaagcgcgt gatcctggcc
    14281 gacgcgaatc tggataaggt cctctccgcg tacaacaagc accgcgacaa gccaatcagg
    14341 gagcaggctg agaatatcat tcatctcttc accctgacga acctcggcgc ccctgctgct
    14401 ttcaagtact tcgacacaac tatcgatcgc aagaggtaca caagcactaa ggaggtcctg
    14461 gacgcgaccc tcatccacca gtcgattacc ggcctctacg agacgcgcat cgacctgtct
    14521 cagctcgggg gcgacaagcg gccagcggcg acgaagaagg cggggcaggc gaagaagaag
    14581 aagtgagctc agagctttcg ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg
    14641 catcagtttc attgcgcaca caccagaatc ctactgagtt tgagtattat ggcattggga
    14701 aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt
    14761 tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt
    14821 ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg
    14881 aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc
    14941 taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta tgcttattca
    15001 ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga atacaagtat
    15061 gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca gaatccttgt
    15121 cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag ttgagtatga
    15181 aaatattttt taatgcattt tatgacttgc caattgattg acaacgaatt cgtaatcatg
    15241 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    15301 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    15361 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    15421 ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc aacatggtgg
    15481 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
    15541 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
    15601 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
    15661 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
    15721 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
    15781 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
    15841 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc
    15901 accagtctct ctctacaaat ctatctctct cgagtctacc atgagcccag aacgacgccc
    15961 ggccgacatc cgccgtgcca ccgaggcgga catgccggcg gtctgcacca tcgtcaacca
    16021 ctacatcgag acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac
    16081 ggacgacctc gtccgtctgc gggagcgcta tccctggctc gtcgccgagg tggacggcga
    16141 ggtcgccggc atcgcctacg cgggcccctg gaaggcacgc aacgcctacg actggacggc
    16201 cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg ggactgggct ccacgctcta
    16261 cacccacctg ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg ctgtcatcgg
    16321 gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc cccgcggcat
    16381 gctgcgggcg gccggcttca agcacgggaa ctggcatgac gtgggtttct ggcagctgga
    16441 cttcagcctg ccggtaccgc cccgtccggt cctgcccgtc accgagattt gactcgagtt
    16501 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
    16561 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
    16621 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa
    16681 ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
    16741 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
    16801 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
    16861 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
    16921 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
    16981 gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga
    17041 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag
    17101 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg
    17161 tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca
    17221 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
    17281 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
    17341 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
    17401 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
    17461 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
    17521 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
    17581 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
    17641 aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat
    17701 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
    17761 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
    17821 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
    17881 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
    17941 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta
    18001 gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    18061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    18121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    18181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    18241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    18301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    18361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    18421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    18481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    18541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    18601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    18661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    18721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    18781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    18841 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
    18901 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
    18961 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
    19021 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    19081 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
    19141 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
    19201 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
    19261 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
    19321 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
    19381 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
    19441 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
    19501 ggctttgggt tcccaatgta cgtgctatcc acaggaaaca gaccttttcg acctttttcc
    19561 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
    19621 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
    19681 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
    19741 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
    19801 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
    19861 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
    19921 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
    19981 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
    20041 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
    20101 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
    20161 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
    20221 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
    20281 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
    20341 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
    20401 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
    20461 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
    20521 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
    20581 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
    20641 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
    20701 tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg cccgttcctc
    20761 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
    20821 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    20881 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    20941 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    21001 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    21061 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    21121 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    21181 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    21241 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    21301 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    21361 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    21421 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    21481 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    21541 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    21601 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    21661 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    21721 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    21781 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    21841 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    21901 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    21961 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    22021 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    22081 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    22141 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    22201 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    22261 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    22321 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    22381 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    22441 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    22501 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    22561 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    22621 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    22681 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    22741 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    22801 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    22861 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    22921 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt
    22981 cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
//
