LOCUS       sequence-495718-       10569 bp    DNA     circular SYN 18-DEC-2025
DEFINITION  Fluorescent reporter gene with miRNA-124 target sequence to repress 
            transgene expression in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard 
            G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, 
            Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, 
            du Pasquier R, Deglon N
  TITLE     The Self-Inactivating KamiCas9 System for the Editing of CNS Disease
            Genes.
  JOURNAL   Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 
            10.1016/j.celrep.2017.08.075.
  PUBMED    28930690
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-19- 19"; volume: "20"; issue: "12"; pages: "2980-2991"
COMMENT     Sequence Label: SIN-cPPT-G1B3-GFP-WPRE-miR124T
FEATURES             Location/Qualifiers
     source          1..10569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3344..3368
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3436..3445
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3442..3444,3445..3447,3448..4158)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3442..3444,3445..3447,3448..4158)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3487..3508)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3748..3767)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4095..4116
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4235..4251)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4262..4286)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4309..4325)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4339..4927
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4392..4412)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4810..4821)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5131..5364
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5389..5407)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5390..5407)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5697..5716)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5930..5949)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6049..6071
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6109..6127)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6195..6299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6300..6368,6369..7160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7331..7919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7820..7839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8073..8090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8160..8180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8165..8494
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8345..8480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9628..9693
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9823..9843
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10268..10402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10305..10324)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10359..10378
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatccg atgtacgggc
     2161 cagatatacg cgttgacatt gattattgac taggatccgg taccgaattc gagctcggta
     2221 ccagatctaa catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc
     2281 ctgagctggc tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac
     2341 ctggcagcat tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca
     2401 ccttggcaca gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc
     2461 ccccctcaaa tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct
     2521 gacagcctgg catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt
     2581 ggcgccaccg gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag
     2641 gggatgccca ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca
     2701 gaagtccaag gacacaaatg ggtgagggga gagctctccc catagctggg ctgcggccca
     2761 accccacccc ctcaggctat gccagggggt gttgccaggg gcacccgatc ttcctcttca
     2821 tgcccagtga atgactcacc ttggcacaga cacaatgttc ggggtgggca cagtgcctgc
     2881 ttcccgccgc accccagccc ccctcaaatg ccttccgaga agcccattga gcaggcccga
     2941 tcttcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg
     3001 cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt
     3061 gagcaggccc gatcttcctc ttcatgccca gtgaatgact caccttggca cagacacaat
     3121 gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca gcccccctca aatgccttcc
     3181 gagaagccca ttgagcaggc ccgggcatcg ccagtctagc ccactccttc ataaagccct
     3241 cgcatcccag gagcgagcag agccagagca ggttggagag gagacgcatc acctccgctg
     3301 ctcgcgggga tcctagtgga tcccccgggc tgcaggaatt atcacaagtt tgtacaaaaa
     3361 agcaggctcc accatgggaa ccaattcagt cgactggatc caggcctaag cttacgcgtc
     3421 ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3481 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3541 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3601 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3661 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3721 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3781 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3841 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3901 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     3961 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4021 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4081 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4141 atggacgagc tgtacaagtc cggactcaga tctcgactag ctagtagcta gctagctagt
     4201 cgagctcaag cttcgaattc gatatcaagc ttatcgatac cgtcgacctc gagatatcta
     4261 gacccagctt tcttgtacaa agtggtgata attcgatatc aagcttatcg ataccgtcga
     4321 cctcgaggga attccgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4381 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4441 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4501 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4561 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4621 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4681 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     4741 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4801 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4861 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4921 tccccgcatc gggaattcga gctcggtacc taatggcatt caccgcgtgc cttaattcga
     4981 atggcattca ccgcgtgcct taaacgcgtt ggcattcacc gcgtgcctta aatgcattgg
     5041 cattcaccgc gtgccttaag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5101 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5161 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5221 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5281 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5341 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
     5401 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     5461 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     5521 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     5581 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     5641 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     5701 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     5761 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     5821 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     5881 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     5941 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     6001 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6061 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     6121 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7441 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7981 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8041 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8101 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     8161 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8221 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8281 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8341 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8401 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8461 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     8521 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     8581 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     8641 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     8701 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     8761 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     8821 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     8881 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     8941 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     9001 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     9061 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     9121 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     9181 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     9241 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     9301 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     9361 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     9421 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     9481 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     9541 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     9601 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     9661 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     9721 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     9781 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     9841 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     9901 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     9961 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
    10021 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
    10081 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
    10141 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
    10201 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    10261 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10321 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10381 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    10441 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    10501 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10561 cttagtagt
//
