LOCUS       sequence-495719-        8134 bp    DNA     circular SYN 18-DEC-2025
DEFINITION  This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain 
            that contains the M1783T mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Gracia B, Montes P, Gutierrez AM, Arun B, Karras GI
  TITLE     Protein-folding chaperones predict structure-function relationships 
            and cancer risk in BRCA1 mutation carriers.
  JOURNAL   Cell Rep. 2024 Feb 27;43(2):113803. doi: 
            10.1016/j.celrep.2024.113803. Epub 2024 Feb 17.
  PUBMED    38368609
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-02-27"; volume: "43(2):113803. doi"; pages: " 
            10.1016/j.celrep.2024.113803. Epub 2024 Feb 17"
COMMENT     Sequence Label: pKlab264-pcDNA3-BRCA1(1314-end)-M1783T-V5-3xFLAG
FEATURES             Location/Qualifiers
     source          1..8134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_RNA        583..658
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        671..919
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     671..691
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        941..1016
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        1035..1338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1339..1542
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1493..1513
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1587..1606
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1587..1605
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1693..1717
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3371..3395)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3397..3438
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3457..3522
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(3566..3583)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3572..3683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3832..4260
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3919..3938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4129..4150
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4269..4289)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4274..4603
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4454..4589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4516..4535
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4652..5677
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4652..5677
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    5807..5928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5844..5863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5898..5917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5977..5993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5977..5993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5990..6012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6001..6017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6025..6031,6032..6049,6050..6055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6070..6091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6208..6225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6379..6967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6459..6478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7138..7929,7930..7998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7761..7780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7999..8103)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtgaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      481 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      541 cttgtggaaa ggacgaaaca ccgctgatgg tgacatcgtc tggttttaga gctagaaata
      601 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      661 ttttcttaag gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
      721 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
      781 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
      841 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      901 gtggaaagga cgaaacaccg catttctggc gctttgcgga gttttagagc tagaaatagc
      961 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     1021 ttactagtgg tacccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1081 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1141 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1201 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     1261 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1321 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     1381 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1441 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1501 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     1561 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
     1621 tagcgtttaa acttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
     1681 ctgcagatat caacaagttt gtacaaaaaa gttggcatgg atcctttctt gattggttct
     1741 tccaaacaaa tgaggcatca gtctgaaagc cagggagttg gtctgagtga caaggaattg
     1801 gtttcagatg atgaagaaag aggaacgggc ttggaagaaa ataatcaaga agagcaaagc
     1861 atggattcaa acttaggtga agcagcatct gggtgtgaga gtgaaacaag cgtctctgaa
     1921 gactgctcag ggctatcctc tcagagtgac attttaacca ctcagcagag ggataccatg
     1981 caacataacc tgataaagct ccagcaggaa atggctgaac tagaagctgt gttagaacag
     2041 catgggagcc agccttctaa cagctaccct tccatcataa gtgactcctc tgcccttgag
     2101 gacctgcgaa atccagaaca aagcacatca gaaaaagcag tattaacttc acagaaaagt
     2161 agtgaatacc ctataagcca gaatccagaa ggcctttctg ctgacaagtt tgaggtgtct
     2221 gcagatagtt ctaccagtaa aaataaagaa ccaggagtgg aaaggtcatc cccttctaaa
     2281 tgcccatcat tagatgatag gtggtacatg cacagttgct ctgggagtct tcagaataga
     2341 aactacccat ctcaagagga gctcattaag gttgttgatg tggaggagca acagctggaa
     2401 gagtctgggc cacacgattt gacggaaaca tcttacttgc caaggcaaga tctagaggga
     2461 accccttacc tggaatctgg aatcagcctc ttctctgatg accctgaatc tgatccttct
     2521 gaagacagag ccccagagtc agctcgtgtt ggcaacatac catcttcaac ctctgcattg
     2581 aaagttcccc aattgaaagt tgcagaatct gcccagggtc cagctgctgc tcatactact
     2641 gatactgctg ggtataatgc aatggaagaa agtgtgagca gggagaagcc agaattgaca
     2701 gcttcaacag aaagggtcaa caaaagaatg tccatggtgg tgtctggcct gaccccagaa
     2761 gaatttatgc tcgtgtacaa gtttgccaga aaacaccaca tcactttaac taatctaatt
     2821 actgaagaga ctactcatgt tgttatgaaa acagatgctg agtttgtgtg tgaacggaca
     2881 ctgaaatatt ttctaggaat tgtgggagga aaatgggtag ttagctattt ctgggtgacc
     2941 cagtctatta aagaaagaaa aatgctgaat gagcatgatt ttgaagtcag aggagatgtg
     3001 gtcaatggaa gaaaccacca aggtccaaag cgagcaagag aatcccagga cagaaagatc
     3061 ttcagggggc tagaaatctg ttgctatggg cccttcacca acatgcccac agatcaactg
     3121 gaatggatgg tacagctgtg tggtgcttct gtggtgaagg agctttcatc attcaccctt
     3181 ggcacaggtg tccacccaat tgtggttgtg cagccagatg cctggacaga ggacaatggc
     3241 ttccatgcaa ttgggcagat gtgtgaggca cctgtggtga cccgagagtg ggtgttggac
     3301 agtgtagcac tctaccagtg ccaggagctg gacacctacc tgatacccca gatcccccac
     3361 agccactact acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc
     3421 ctcggtctcg attctacagg ctcgggctcg acctcggact acaaagacca tgacggtgat
     3481 tataaagatc atgacatcga ctacaaggat gacgatgaca agtgataact cgagtctaga
     3541 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3601 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3661 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3721 ggggtggggc aggacagcaa ggggaagaca atagcaggca tgctggggat gcggtgggct
     3781 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     3841 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3901 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3961 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4021 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4081 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4141 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4201 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4261 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4321 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4381 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4441 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4501 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4561 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4621 tatatccatt ttcggatctg atcagcacgt gatgaaaaag cctgaactca ccgcgacgtc
     4681 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     4741 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     4801 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     4861 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     4921 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     4981 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5041 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5101 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5161 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5221 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5281 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5341 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5401 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5461 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5521 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     5581 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     5641 ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtgctacgag atttcgattc
     5701 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5761 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5821 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5881 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5941 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6001 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6061 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6121 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6781 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8101 gcgcacattt ccccgaaaag tgccacctga cgtc
//
