LOCUS       sequence-49576-e         334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Perez-Pinera P, Ousterout DG, Brown MT, Gersbach CA
  TITLE     Gene targeting to the ROSA26 locus directed by engineered zinc 
            finger nucleases.
  JOURNAL   Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14.
  PUBMED    22169954
REFERENCE   2  (bases 1 to 334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-04-1"; volume: "40(8)"; pages: "3741-52. 
            Epub 2011 Dec 14"
COMMENT     Sequence Label: pCMV-RosaR4 KKR mutations
FEATURES             Location/Qualifiers
     source          1..334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        184..202
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     184..201
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     215..234
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             281..307
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg
       61 aacgcggatt ccccgtgcca agagtgacgt aagtaccgcc tatagagtct ataggcccac
      121 ccccttggct tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat
      181 acgatttagg tgacactata gaataacatc cactttgcct ttctctccac aggtgtccac
      241 tcccaggtcc aactgcacct cggttctatc gattgacatg tacccatacg atgtcccaga
      301 ctacgcgaat tcccctggga tcccaggcat gggg
//
