LOCUS       sequence-495860-        9462 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for human GPR124 lacking the ICD, N-terminal 
            3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: p3xFLAG-CMV-9-GPR124 Delta-ICD
FEATURES             Location/Qualifiers
     source          1..9462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(93..110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1023..1814,1815..1883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1646..1665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1884..1988)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2102..2121)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2329..2350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2613..2635
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2627..2644
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2628..2644
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        2805..3184
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3185..3388
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3339..3359
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3419..3463
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             3464..3529
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    6690..7312
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6889..6908)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(7336..7356)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7354..7652
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7706..8500
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7760..7779)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8370..8389
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9152..9286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9189..9208)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9243..9262
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9291..9310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9292..9310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9324..9340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9324..9340)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9337..9359)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9348..9364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9372..9378,9379..9396,9397..9402))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9417..9438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
       61 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      241 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1141 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1981 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     2041 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2101 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2161 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2221 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2281 cctttgacgt tggagtccac gttctttaag agtccactat taaagaacgt ggactcttgt
     2341 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     2401 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2461 ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga
     2521 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     2581 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     2641 cagtgccaag ctgatctata cattgaatca atattggcaa ttagccatat tagtcattgg
     2701 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
     2761 tatgtacatt tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac
     2821 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     2881 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2941 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3001 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3061 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3121 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3181 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
     3241 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3301 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
     3361 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagaatt aattcaccat
     3421 gtctgcactt ctgatcctag ctcttgttgg agctgcagtt gctgactaca aagaccatga
     3481 cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagc ttgcggccgc
     3541 gaattcagcg cccggctgcc cgctatccat ccgcagctgc aagtgctcgg gggagcggcc
     3601 caaggggctg agcggcggcg tccctggccc ggctcggcgg agggtggtgt gcagcggcgg
     3661 ggacctcccg gagcctcccg agcccggcct tctgcctaac ggcaccgtta ccctgctctt
     3721 gagcaataac aagatcacgg ggctccgcaa tggctccttc ctgggactgt cactgctgga
     3781 gaagctggac ctgaggaaca acatcatcag cacagtgcag ccgggcgcct tcctgggcct
     3841 gggggagctg aagcgtttag atctctccaa caaccggatt ggctgtctca cctccgagac
     3901 cttccagggc ctccccaggc ttctccgact aaacatatct ggaaacatct tctccagtct
     3961 gcaacctggg gtctttgatg agctgccagc ccttaaggtt gtggacttgg gcaccgagtt
     4021 cctgacctgt gactgccacc tgcgctggct gctgccctgg gcccagaatc gctccctgca
     4081 gctgtcggaa cacacgctct gtgcttaccc cagtgccctg catgctcagg ccctgggcag
     4141 cctccaggag gcccagctct gctgcgaggg ggccctggag ctgcacacac accacctcat
     4201 cccgtcccta cgccaagtgg tgttccaggg ggatcggctg cccttccagt gctctgccag
     4261 ctacctgggc aacgacaccc gcatccgctg gtaccacaac cgagcccctg tggagggtga
     4321 tgagcaggcg ggcatcctcc tggccgagag cctcatccac gactgcacct tcatcaccag
     4381 tgagctgacg ctgtctcaca tcggcgtgtg ggcctcaggc gagtgggagt gcaccgtgtc
     4441 catggcccaa ggcaacgcca gcaagaaggt ggagatcgtg gtgctggaga cctctgcctc
     4501 ctactgcccc gccgagcgtg ttgccaacaa ccgcggggac ttcaggtggc cccgaactct
     4561 ggctggcatc acagcctacc agtcctgcct gcagtatccc ttcacctcag tgcccctggg
     4621 cgggggtgcc ccgggcaccc gagcctcccg ccggtgtgac cgtgccggcc gctgggagcc
     4681 aggggactac tcccactgtc tctacaccaa cgacatcacc agggtgctgt acaccttcgt
     4741 gctgatgccc atcaatgcct ccaatgcgct gaccctggct caccagctgc gcgtgtacac
     4801 agccgaggcc gctagctttt cagacatgat ggatgtagtc tatgtggctc agatgatcca
     4861 gaaatttttg ggttatgtcg accagatcaa agagctggta gaggtgatgg tggacatggc
     4921 cagcaacctg atgctggtgg acgagcacct gctgtggctg gcccagcgcg aggacaaggc
     4981 ctgcagccgc atcgtgggtg ccctggagcg cattgggggg gccgccctca gcccccatgc
     5041 ccagcacatc tcagtgaatg cgaggaacgt ggcattggag gcctacctca tcaagccgca
     5101 cagctacgtg ggcctgacct gcacagcctt ccagaggagg gagggagggg tgccgggcac
     5161 acggccagga agccctggcc agaacccccc acctgagccc gagcccccag ctgaccagca
     5221 gctccgcttc cgctgcacca ccgggaggcc caatgtttct ctgtcgtcct tccacatcaa
     5281 gaacagcgtg gccctggcct ccatccagct gcccccgagt ctattctcat cccttccggc
     5341 tgccctggct cccccggtgc ccccagactg caccctgcaa ctgctcgtct tccgaaatgg
     5401 ccgcctcttc cacagccaca gcaacacctc ccgccctgga gctgctgggc ctggcaagag
     5461 gcgtggcgtg gccacccccg tcatcttcgc aggaaccagt ggctgtggcg tgggaaacct
     5521 gacagagcca gtggccgttt cgctgcggca ctgggctgag ggagccgaac ctgtggccgc
     5581 ttggtggagc caggaggggc ccggggaggc tgggggctgg acctcggagg gctgccagct
     5641 ccgctccagc cagcccaatg tcagcgccct gcactgccag cacttgggca atgtggccgt
     5701 gctcatggag ctgagcgcct ttcccaggga ggtggggggc gccggggcag ggctgcaccc
     5761 cgtggtatac ccctgcacgg ccttgctgct gctctgcctc ttcgccacca tcatcaccta
     5821 catcctcaac cacagctcca tccgtgtgtc ccggaaaggc tggcacatgc tgctgaactt
     5881 gtgcttccac atagccatga cctctgctgt ctttgcgggg ggcatcacac tcaccaacta
     5941 ccagatggtc tgccaggcgg tgggcatcac cctgcactac tcctccctat ccacgctgct
     6001 ctggatgggc gtgaaggcgc gagtgctcca taaggagctc acctggaggg caccccctcc
     6061 gcaagaaggg gaccccgctc tgcctactcc cagtcctatg ctccggttct atttgatcgc
     6121 tggagggatt ccactcatta tctgtggcat cacagctgca gtcaacatcc acaactaccg
     6181 ggaccacagc ccctactgct ggctggtgtg gcgtccaagc cttggcgcct tctacatccc
     6241 tgtggctttg attctgctca tcacctggat ctatttcctg tgcgccgggc tacgcttacg
     6301 gggtcctctg gcacagaacc ccaaggcggg caacagcagg gcctccctgg aggcagggga
     6361 ggagctgagg ggttccacca ggctcagggg cagcggcccc ctcctgagtg actcaggttc
     6421 ccttcttgct actgggagcg cgcgagtggg gacgcccggg cccccggagg atggtgacag
     6481 cctctattct ccgggagtcc agctaggggc gctggtgacc acgcacttcc tgtacttggc
     6541 catgtgggcc tgcggggctc tggcagtgtc ccagcgctgg ctgccccggg tggtgtgcag
     6601 ctgcttgtac ggggtggcag cctccgccct gggcctcttc gtcttcactc accactgtgc
     6661 caggcggagg gacgtgagat aaggatcccg ggtggcatcc ctgtgacccc tccccagtgc
     6721 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
     6781 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
     6841 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
     6901 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
     6961 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca
     7021 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
     7081 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
     7141 gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag caggaggacg
     7201 tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg agttgcttgc
     7261 ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc
     7321 ggccagcttg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7381 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7501 tcccgcccct aactccgccc atcccgcctc cgctaactcc gcccagttcc gcccattctc
     7561 cgccccatgg ctgactaatt ttttttattt atgcaggccg aggccgcctc ggcctctgag
     7621 ctattccaga agtagtgagg aggctttttt ggaggaattg atcagcttgg gatctgatca
     7681 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     7741 ggctgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     7801 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     7861 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     7921 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     7981 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     8041 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     8101 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     8161 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     8221 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     8281 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     8341 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     8401 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     8461 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     8521 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     8581 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8641 ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc gagttggttc
     8701 agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa atccgtcggc
     8761 atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca ggagtgggga
     8821 ggcacgatgg ccgctttggt cgacccggac gggacgctcc tgcgcctgat acagaacgaa
     8881 ttgcttgcag gcatctcatg agtgtgtctt cccgttttcc gcctgaggtc actgcgtgga
     8941 tggagcgctg gcgcctgctg cgcgacggcg agctgctcac cacccactcg ccaagctgga
     9001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgccgatca taatcagcca
     9061 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     9121 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     9181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9241 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaatt ccctatagtg
     9301 agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9361 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9421 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ct
//
