LOCUS       sequence-495862-        8920 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for secreted human RECK, C-terminal 6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: pcDNA3.1-Ig-kappa SP-sRECK-His
FEATURES             Location/Qualifiers
     source          1..8920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      899..908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     905..964
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             3743..3760
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3775..4470
                     /codon_start=1
                     /product="CH2 and CH3 domains of the mouse IgG2a heavy 
                     chain plus the hinge region"
                     /label=Fc tag
                     /translation="PRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVT
                     CVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF
                     KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIY
                     VEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHT
                     TKSFSRTPGK"
     primer_bind     complement(4511..4528)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4517..4741
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4787..5215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4874..4893)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5084..5105
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5224..5244)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5229..5558
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5409..5544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5471..5490
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5625..6419
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5679..5698)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6289..6308
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6593..6714
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6630..6649)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6684..6703
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6763..6779)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6763..6779)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6776..6798)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6787..6803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6811..6817,6818..6835,6836..6841))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6856..6877
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6994..7011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7165..7753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7245..7264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7924..8715,8716..8784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8547..8566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8785..8889)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
      961 tggtgacgcg gcccagccgg ccgaggtggc agggggcctg gctccgggca gtgcgggtgc
     1021 attgtgttgt aatcattcaa aggataacca aatgtgccgt gatgtatgtg aacagatttt
     1081 ctcctcaaaa agtgaatccc gactaaaaca tctgttgcag cgagccccag attattgccc
     1141 agagacaatg gttgaaattt ggaattgtat gaattcatct ttgccaggtg tgtttaagaa
     1201 gtctgatggc tgggttggct taggctgctg tgaactggct attgccttgg agtgtcgaca
     1261 ggcatgcaag caggcatctt caaagaatga tatttccaaa gtttgcagaa aagaatatga
     1321 gaatgctctt ttcagttgca ttagcagaaa tgaaatgggc tcggtttgtt gcagttatgc
     1381 aggtcatcac acaaactgcc gagaatactg tcaagccatt tttcgaacag actcttctcc
     1441 tggtccatct cagataaaag cagtggaaaa ttattgcgcc tctattagtc cacaattaat
     1501 acattgtgtg aacaattata ctcaatctta tccaatgagg aacccaacgg atagtttata
     1561 ttgctgtgac agagctgaag accatgcttg ccaaaatgcc tgcaagagaa tcctgatgtc
     1621 caagaaaacg gaaatggaga ttgttgatgg tctcatcgag ggttgtaaga cccagccctt
     1681 gcctcaagat cctctttggc aatgttttct tgaaagctca caatctgttc accctggagt
     1741 cactgtacac cctcctccct ctacaggcct cgatggggct aaattgcatt gttgttctaa
     1801 agcaaacact tcaacatgta gggaactctg cactaaactt tacagcatga gctggggcaa
     1861 tacacagagt tggcaagagt ttgatcgctt ttgtgaatat aatccagtgg aagtgtccat
     1921 gttgacctgt ttagcggatg tccgggaacc ttgccagttg ggctgtagaa accttactta
     1981 ctgtactaat tttaacaaca ggccaacaga acttttcagg agttgtaatg cacagtcaga
     2041 tcaaggagcc atgaatgaca tgaagttgtg ggagaaagga agcataaaga tgccatttat
     2101 caatatacct gttcttgata ttaaaaagtg ccagccagag atgtggaaag caatagcttg
     2161 ttcactgcag attaaacctt gtcatagtaa atctcgggga agtattattt gcaaatcaga
     2221 ttgtgtggag attcttaaaa aatgtggaga ccagaacaaa ttccctgaag accacacagc
     2281 tgaaagtatt tgtgagcttc tgtcacctac agatgatctg aagaattgta tacctttgga
     2341 tacatacctc aggccaagta ctttaggtaa cattgtagaa gaagtgactc atccctgtaa
     2401 cccaaatcct tgccctgcca atgagctctg tgaagtaaac cgaaaaggat gtccatctgg
     2461 agatccctgt cttccatact tttgtgttca aggttgcaaa ctgggagaag cttctgattt
     2521 cattgtccgt caagggacac taatccaggt gccatcatct gcaggggaag ttggttgtta
     2581 taaaatctgt tcatgtggac aaagtggact cttagaaaac tgtatggaaa tgcactgtat
     2641 agacctccag aagtcttgta ttgttggagg aaaaagaaaa agtcatggaa catcctttag
     2701 tattgactgc aatgtctgtt cttgttttgc tggcaatttg gtgtgctcta cccgcctttg
     2761 cctcagtgag cacagttcag aagatgaccg tcgtaccttc acaggtctgc cctgtaactg
     2821 tgcagatcag tttgtccctg tatgtgggca gaatgggcgc acttacccca gtgcctgcat
     2881 tgctcgctgt gtgggcctcc aagaccatca gtttgagttt ggatcatgca tgtcaaagga
     2941 tccatgtaat cctaatccct gccaaaaaaa ccaaaggtgc atacccaaac cacaggtctg
     3001 cctgacgact tttgataaat ttggatgtag ccagtatgag tgtgtaccaa gacagctcgc
     3061 gtgtgaccag gtccaagatc ctgtttgtga cacagaccac atggagcaca acaatctctg
     3121 cactttatac caaagaggaa aaagcctctc ttacaaaggt ccctgccagc ccttttgcag
     3181 agcaaccgag cccgtatgtg ggcacaatgg tgagacctac agcagtgtgt gtgctgccta
     3241 ctcggatcgc gtggcagtcg attactatgg ggactgccag gccgtcggag tcctctcaga
     3301 gcacagctcc gtcgccgagt gtgcttctgt caagtgtcct tcgctcttgg cagctggatg
     3361 caaacccatc atcccaccgg gtgcttgttg cccattatgt gctgggatgt taagagtttt
     3421 atttgacaaa gaaaaactgg atactattgc taaggtaaca aataaaaagc caataacagt
     3481 tctggaaata cttcagaaaa tccgcatgca cgtgtctgtc ccacagtgtg atgtgtttgg
     3541 atacttcagc attgaatcag aaattgtgat cctgatcatt cccgtcgatc actatccaaa
     3601 agctctgcag attgaagcct gcaataaaga agcagagaag attgagtccc ttatcaactc
     3661 tgacagcccg actttggcgt cccatgtccc tctctctgcc ctcatcattt cccaggtaca
     3721 ggtctccagc agtgtgccat cgcatcacca tcaccatcac taactcgact agagcccaga
     3781 gggcccacaa tcaagccctg tcctccatgc aaatgcccag cacctaacct cttgggtgga
     3841 ccatccgtct tcatcttccc tccaaagatc aaggatgtac tcatgatctc cctgagcccc
     3901 atagtcacat gtgtggtggt ggatgtgagc gaggatgacc cagatgtcca gatcagctgg
     3961 tttgtgaaca acgtggaagt acacacagct cagacacaaa cccatagaga ggattacaac
     4021 agtactctcc gggtggtcag tgccctcccc atccagcacc aggactggat gagtggcaag
     4081 gagttcaaat gcaaggtcaa caacaaagac ctcccagcgc ccatcgagag aaccatctca
     4141 aaacccaaag ggtcagtaag agctccacag gtatatgtct tgcctccacc agaagaagag
     4201 atgactaaga aacaggtcac tctgacctgc atggtcacag acttcatgcc tgaagacatt
     4261 tacgtggagt ggaccaacaa cgggaaaaca gagctaaact acaagaacac tgaaccagtc
     4321 ctggactctg atggttctta cttcatgtac agcaagctga gagtggaaaa gaagaactgg
     4381 gtggaaagaa atagctactc ctgttcagtg gtccacgagg gtctgcacaa tcaccacacg
     4441 actaagagct tctcccggac tccgggtaaa tgactcgagt ctagagggcc cgtttaaacc
     4501 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4561 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4621 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4681 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4741 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4801 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4861 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4921 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4981 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5041 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5101 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5161 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5221 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5281 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5341 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5401 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5461 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5521 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5581 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5641 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5701 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5761 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     5821 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5881 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5941 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6001 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6061 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6121 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6181 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6241 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6301 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6361 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6421 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6481 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6541 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6601 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6661 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6721 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6781 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6841 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6961 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7021 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7081 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7141 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7201 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7261 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7321 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7381 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7441 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7501 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7561 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7621 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7681 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7741 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7801 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7861 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7921 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7981 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8041 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8101 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8161 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8221 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8281 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8341 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8401 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8461 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8521 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8581 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8641 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8701 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8761 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8821 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8881 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
