LOCUS       sequence-495864-       10245 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for human GPR124, N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 10245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: p3xFLAG-CMV-9-GPR124
FEATURES             Location/Qualifiers
     source          1..10245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             932..976
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             977..1042
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4986..5608
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5185..5204)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5632..5652)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5650..5948
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6002..6796
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6056..6075)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6666..6685
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7448..7582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7485..7504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7539..7558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7587..7606)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7588..7606)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7620..7636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7620..7636)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7633..7655)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7644..7660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7668..7674,7675..7692,7693..7698))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7713..7734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7851..7868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8022..8610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8102..8121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8781..9572,9573..9641))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9404..9423
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9642..9746)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9860..9879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10087..10108
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaattcac catgtctgca cttctgatcc tagctcttgt
      961 tggagctgca gttgctgact acaaagacca tgacggtgat tataaagatc atgacatcga
     1021 ttacaaggat gacgatgaca agcttgcggc cgcgaattca gcgcccggct gcccgctatc
     1081 catccgcagc tgcaagtgct cgggggagcg gcccaagggg ctgagcggcg gcgtccctgg
     1141 cccggctcgg cggagggtgg tgtgcagcgg cggggacctc ccggagcctc ccgagcccgg
     1201 ccttctgcct aacggcaccg ttaccctgct cttgagcaat aacaagatca cggggctccg
     1261 caatggctcc ttcctgggac tgtcactgct ggagaagctg gacctgagga acaacatcat
     1321 cagcacagtg cagccgggcg ccttcctggg cctgggggag ctgaagcgtt tagatctctc
     1381 caacaaccgg attggctgtc tcacctccga gaccttccag ggcctcccca ggcttctccg
     1441 actaaacata tctggaaaca tcttctccag tctgcaacct ggggtctttg atgagctgcc
     1501 agcccttaag gttgtggact tgggcaccga gttcctgacc tgtgactgcc acctgcgctg
     1561 gctgctgccc tgggcccaga atcgctccct gcagctgtcg gaacacacgc tctgtgctta
     1621 ccccagtgcc ctgcatgctc aggccctggg cagcctccag gaggcccagc tctgctgcga
     1681 gggggccctg gagctgcaca cacaccacct catcccgtcc ctacgccaag tggtgttcca
     1741 gggggatcgg ctgcccttcc agtgctctgc cagctacctg ggcaacgaca cccgcatccg
     1801 ctggtaccac aaccgagccc ctgtggaggg tgatgagcag gcgggcatcc tcctggccga
     1861 gagcctcatc cacgactgca ccttcatcac cagtgagctg acgctgtctc acatcggcgt
     1921 gtgggcctca ggcgagtggg agtgcaccgt gtccatggcc caaggcaacg ccagcaagaa
     1981 ggtggagatc gtggtgctgg agacctctgc ctcctactgc cccgccgagc gtgttgccaa
     2041 caaccgcggg gacttcaggt ggccccgaac tctggctggc atcacagcct accagtcctg
     2101 cctgcagtat cccttcacct cagtgcccct gggcgggggt gccccgggca cccgagcctc
     2161 ccgccggtgt gaccgtgccg gccgctggga gccaggggac tactcccact gtctctacac
     2221 caacgacatc accagggtgc tgtacacctt cgtgctgatg cccatcaatg cctccaatgc
     2281 gctgaccctg gctcaccagc tgcgcgtgta cacagccgag gccgctagct tttcagacat
     2341 gatggatgta gtctatgtgg ctcagatgat ccagaaattt ttgggttatg tcgaccagat
     2401 caaagagctg gtagaggtga tggtggacat ggccagcaac ctgatgctgg tggacgagca
     2461 cctgctgtgg ctggcccagc gcgaggacaa ggcctgcagc cgcatcgtgg gtgccctgga
     2521 gcgcattggg ggggccgccc tcagccccca tgcccagcac atctcagtga atgcgaggaa
     2581 cgtggcattg gaggcctacc tcatcaagcc gcacagctac gtgggcctga cctgcacagc
     2641 cttccagagg agggagggag gggtgccggg cacacggcca ggaagccctg gccagaaccc
     2701 cccacctgag cccgagcccc cagctgacca gcagctccgc ttccgctgca ccaccgggag
     2761 gcccaatgtt tctctgtcgt ccttccacat caagaacagc gtggccctgg cctccatcca
     2821 gctgcccccg agtctattct catcccttcc ggctgccctg gctcccccgg tgcccccaga
     2881 ctgcaccctg caactgctcg tcttccgaaa tggccgcctc ttccacagcc acagcaacac
     2941 ctcccgccct ggagctgctg ggcctggcaa gaggcgtggc gtggccaccc ccgtcatctt
     3001 cgcaggaacc agtggctgtg gcgtgggaaa cctgacagag ccagtggccg tttcgctgcg
     3061 gcactgggct gagggagccg aacctgtggc cgcttggtgg agccaggagg ggcccgggga
     3121 ggctgggggc tggacctcgg agggctgcca gctccgctcc agccagccca atgtcagcgc
     3181 cctgcactgc cagcacttgg gcaatgtggc cgtgctcatg gagctgagcg cctttcccag
     3241 ggaggtgggg ggcgccgggg cagggctgca ccccgtggta tacccctgca cggccttgct
     3301 gctgctctgc ctcttcgcca ccatcatcac ctacatcctc aaccacagct ccatccgtgt
     3361 gtcccggaaa ggctggcaca tgctgctgaa cttgtgcttc cacatagcca tgacctctgc
     3421 tgtctttgcg gggggcatca cactcaccaa ctaccagatg gtctgccagg cggtgggcat
     3481 caccctgcac tactcctccc tatccacgct gctctggatg ggcgtgaagg cgcgagtgct
     3541 ccataaggag ctcacctgga gggcaccccc tccgcaagaa ggggaccccg ctctgcctac
     3601 tcccagtcct atgctccggt tctatttgat cgctggaggg attccactca ttatctgtgg
     3661 catcacagct gcagtcaaca tccacaacta ccgggaccac agcccctact gctggctggt
     3721 gtggcgtcca agccttggcg ccttctacat ccctgtggct ttgattctgc tcatcacctg
     3781 gatctatttc ctgtgcgccg ggctacgctt acggggtcct ctggcacaga accccaaggc
     3841 gggcaacagc agggcctccc tggaggcagg ggaggagctg aggggttcca ccaggctcag
     3901 gggcagcggc cccctcctga gtgactcagg ttcccttctt gctactggga gcgcgcgagt
     3961 ggggacgccc gggcccccgg aggatggtga cagcctctat tctccgggag tccagctagg
     4021 ggcgctggtg accacgcact tcctgtactt ggccatgtgg gcctgcgggg ctctggcagt
     4081 gtcccagcgc tggctgcccc gggtggtgtg cagctgcttg tacggggtgg cagcctccgc
     4141 cctgggcctc ttcgtcttca ctcaccactg tgccaggcgg agggacgtga gagcctcgtg
     4201 gcgcgcctgc tgcccccctg cctctcccgc ggccccccat gccccgcccc gggccctgcc
     4261 cgccgccgca gaggacggtt ccccggtgtt cggggagggg cccccctccc tcaagtcctc
     4321 cccaagcggc agcagcggcc atccgctggc tctgggcccc tgcaagctca ccaacctgca
     4381 gctggcccag agtcaggtgt gcgaggcggg ggcggcggcc ggcggggaag gagagccgga
     4441 gccggcgggc acccggggaa acctcgccca ccgccacccc aacaacgtgc accacgggcg
     4501 tcgggcgcac aagagccggg ccaagggaca ccgcgcgggg gaggcctgcg gcaagaaccg
     4561 gctcaaggcc ctgcgcgggg gcgcggcggg ggcgctggag ctgctgtcca gcgagagcgg
     4621 tagtctgcac aacagcccca ccgacagcta cctgggcagc agccgcaaca gcccgggcgc
     4681 cggcctgcag ctggaaggcg agcccatgct cacgccgtcc gagggcagcg acaccagcgc
     4741 cgcgccgctt tctgaggcgg gccgggcagg ccagcgccgc agcgccagcc gcgacagtct
     4801 caagggcggc ggcgcgctgg agaaggagag ccatcgccgc tcgtacccgc tcaacgccgc
     4861 cagcctaaac ggcgccccca aggggggcaa gtacgacgac gtcaccctga tgggcgcgga
     4921 ggtagccagc ggcggctgca tgaagaccgg actctggaag agcgaaacta ccgtctaagg
     4981 atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     5041 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     5101 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     5161 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     5221 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     5281 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     5341 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     5401 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     5461 ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg
     5521 gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg
     5581 ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt
     5641 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5701 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5761 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5821 cgcctccgct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5881 ttatttatgc aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5941 ttttttggag gaattgatca gcttgggatc tgatcaagag acaggatgag gatcgtttcg
     6001 catgattgaa caagatggat tgcacgcagg ttctccggct gcttgggtgg agaggctatt
     6061 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     6121 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     6181 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     6241 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     6301 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     6361 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6421 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6481 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     6541 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6601 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6661 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6721 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6781 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     6841 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6901 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6961 gcccaccccg ggctcgatcc cctcgcgagt tggttcagct gctgcctgag gctggacgac
     7021 ctcgcggagt tctaccggca gtgcaaatcc gtcggcatcc aggaaaccag cagcggctat
     7081 ccgcgcatcc atgcccccga actgcaggag tggggaggca cgatggccgc tttggtcgac
     7141 ccggacggga cgctcctgcg cctgatacag aacgaattgc ttgcaggcat ctcatgagtg
     7201 tgtcttcccg ttttccgcct gaggtcactg cgtggatgga gcgctggcgc ctgctgcgcg
     7261 acggcgagct gctcaccacc cactcgccaa gctggaaccg taaaaaggcc gcgttgctgg
     7321 cgtttttcca taggctccgc cgatcataat cagccatacc acatttgtag aggttttact
     7381 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     7441 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7501 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7561 tgtatcttat catgtctgga tcaattccct atagtgagtc gtattaaatt cgtaatcatg
     7621 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7681 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7741 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7801 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7861 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7921 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7981 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8041 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8101 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8161 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8221 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8281 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8341 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8401 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8461 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8521 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8581 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8641 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8701 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8761 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8821 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8881 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8941 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9001 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9061 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9121 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9181 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9241 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9301 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9361 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9421 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9481 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9541 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9601 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9661 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9721 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     9781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9841 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9901 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9961 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    10021 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaagag tccactatta
    10081 aagaacgtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    10141 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    10201 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aattt
//
