LOCUS       sequence-495865-        9248 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for human RECK, N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 9248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: p3xFLAG-CMV-9-RECK
FEATURES             Location/Qualifiers
     source          1..9248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             932..976
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             977..1042
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    3989..4611
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4188..4207)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4635..4655)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4653..4951
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5005..5799
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5059..5078)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5669..5688
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6451..6585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6488..6507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6542..6561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6590..6609)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6591..6609)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6623..6639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6623..6639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6636..6658)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6647..6663
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6671..6677,6678..6695,6696..6701))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6716..6737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6854..6871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7025..7613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7105..7124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7784..8575,8576..8644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8407..8426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8645..8749)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8863..8882)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9090..9111
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaattcac catgtctgca cttctgatcc tagctcttgt
      961 tggagctgca gttgctgact acaaagacca tgacggtgat tataaagatc atgacatcga
     1021 ttacaaggat gacgatgaca agcttgcggc cgctgaggtg gcagggggcc tggctccggg
     1081 cagtgcgggt gcattgtgtt gtaatcattc aaaggataac caaatgtgcc gtgatgtatg
     1141 tgaacagatt ttctcctcaa aaagtgaatc ccgactaaaa catctgttgc agcgagcccc
     1201 agattattgc ccagagacaa tggttgaaat ttggaattgt atgaattcat ctttgccagg
     1261 tgtgtttaag aagtctgatg gctgggttgg cttaggctgc tgtgaactgg ctattgcctt
     1321 ggagtgtcga caggcatgca agcaggcatc ttcaaagaat gatatttcca aagtttgcag
     1381 aaaagaatat gagaatgctc ttttcagttg cattagcaga aatgaaatgg gctcggtttg
     1441 ttgcagttat gcaggtcatc acacaaactg ccgagaatac tgtcaagcca tttttcgaac
     1501 agactcttct cctggtccat ctcagataaa agcagtggaa aattattgcg cctctattag
     1561 tccacaatta atacattgtg tgaacaatta tactcaatct tatccaatga ggaacccaac
     1621 ggatagttta tattgctgtg acagagctga agaccatgct tgccaaaatg cctgcaagag
     1681 aatcctgatg tccaagaaaa cggaaatgga gattgttgat ggtctcatcg agggttgtaa
     1741 gacccagccc ttgcctcaag atcctctttg gcaatgtttt cttgaaagct cacaatctgt
     1801 tcaccctgga gtcactgtac accctcctcc ctctacaggc ctcgatgggg ctaaattgca
     1861 ttgttgttct aaagcaaaca cttcaacatg tagggaactc tgcactaaac tttacagcat
     1921 gagctggggc aatacacaga gttggcaaga gtttgatcgc ttttgtgaat ataatccagt
     1981 ggaagtgtcc atgttgacct gtttagcgga tgtccgggaa ccttgccagt tgggctgtag
     2041 aaaccttact tactgtacta attttaacaa caggccaaca gaacttttca ggagttgtaa
     2101 tgcacagtca gatcaaggag ccatgaatga catgaagttg tgggagaaag gaagcataaa
     2161 gatgccattt atcaatatac ctgttcttga tattaaaaag tgccagccag agatgtggaa
     2221 agcaatagct tgttcactgc agattaaacc ttgtcatagt aaatctcggg gaagtattat
     2281 ttgcaaatca gattgtgtgg agattcttaa aaaatgtgga gaccagaaca aattccctga
     2341 agaccacaca gctgaaagta tttgtgagct tctgtcacct acagatgatc tgaagaattg
     2401 tatacctttg gatacatacc tcaggccaag tactttaggt aacattgtag aagaagtgac
     2461 tcatccctgt aacccaaatc cttgccctgc caatgagctc tgtgaagtaa accgaaaagg
     2521 atgtccatct ggagatccct gtcttccata cttttgtgtt caaggttgca aactgggaga
     2581 agcttctgat ttcattgtcc gtcaagggac actaatccag gtgccatcat ctgcagggga
     2641 agttggttgt tataaaatct gttcatgtgg acaaagtgga ctcttagaaa actgtatgga
     2701 aatgcactgt atagacctcc agaagtcttg tattgttgga ggaaaaagaa aaagtcatgg
     2761 aacatccttt agtattgact gcaatgtctg ttcttgtttt gctggcaatt tggtgtgctc
     2821 tacccgcctt tgcctcagtg agcacagttc agaagatgac cgtcgtacct tcacaggtct
     2881 gccctgtaac tgtgcagatc agtttgtccc tgtatgtggg cagaatgggc gcacttaccc
     2941 cagtgcctgc attgctcgct gtgtgggcct ccaagaccat cagtttgagt ttggatcatg
     3001 catgtcaaag gatccatgta atcctaatcc ctgccaaaaa aaccaaaggt gcatacccaa
     3061 accacaggtc tgcctgacga cttttgataa atttggatgt agccagtatg agtgtgtacc
     3121 aagacagctc gcgtgtgacc aggtccaaga tcctgtttgt gacacagacc acatggagca
     3181 caacaatctc tgcactttat accaaagagg aaaaagcctc tcttacaaag gtccctgcca
     3241 gcccttttgc agagcaaccg agcccgtatg tgggcacaat ggtgagacct acagcagtgt
     3301 gtgtgctgcc tactcggatc gcgtggcagt cgattactat ggggactgcc aggccgtcgg
     3361 agtcctctca gagcacagct ccgtcgccga gtgtgcttct gtcaagtgtc cttcgctctt
     3421 ggcagctgga tgcaaaccca tcatcccacc gggtgcttgt tgcccattat gtgctgggat
     3481 gttaagagtt ttatttgaca aagaaaaact ggatactatt gctaaggtaa caaataaaaa
     3541 gccaataaca gttctggaaa tacttcagaa aatccgcatg cacgtgtctg tcccacagtg
     3601 tgatgtgttt ggatacttca gcattgaatc agaaattgtg atcctgatca ttcccgtcga
     3661 tcactatcca aaagctctgc agattgaagc ctgcaataaa gaagcagaga agattgagtc
     3721 ccttatcaac tctgacagcc cgactttggc gtcccatgtc cctctctctg ccctcatcat
     3781 ttcccaggta caggtctcca gcagtgtgcc atcggccggt gtcagggcca ggccttcttg
     3841 ccactccctc ctccttcccc tcagcttggg ccttgccttg cacttgctct ggacatataa
     3901 ctgactgccc acggaaagtg cagaatgctc ctccacctca ctctcctgcc agatctgata
     3961 tcggtaccag tcgactctag aggatcccgg gtggcatccc tgtgacccct ccccagtgcc
     4021 tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag
     4081 ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt
     4141 atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc
     4201 aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc
     4261 gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag
     4321 ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa
     4381 ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg
     4441 aaccactgct cccttccctg tccttctgat tttaaaataa ctataccagc aggaggacgt
     4501 ccagacacag cataggctac ctggccatgc ccaaccggtg ggacatttga gttgcttgct
     4561 tggcactgtc ctctcatgcg ttgggtccac tcagtagatg cctgttgaat tgggtacgcg
     4621 gccagcttgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4681 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4741 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4801 cccgccccta actccgccca tcccgcctcc gctaactccg cccagttccg cccattctcc
     4861 gccccatggc tgactaattt tttttattta tgcaggccga ggccgcctcg gcctctgagc
     4921 tattccagaa gtagtgagga ggcttttttg gaggaattga tcagcttggg atctgatcaa
     4981 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5041 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5101 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5161 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5221 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5281 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5341 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5401 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5461 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5521 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     5581 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5641 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5701 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     5761 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     5821 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     5881 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5941 gggatctcat gctggagttc ttcgcccacc ccgggctcga tcccctcgcg agttggttca
     6001 gctgctgcct gaggctggac gacctcgcgg agttctaccg gcagtgcaaa tccgtcggca
     6061 tccaggaaac cagcagcggc tatccgcgca tccatgcccc cgaactgcag gagtggggag
     6121 gcacgatggc cgctttggtc gacccggacg ggacgctcct gcgcctgata cagaacgaat
     6181 tgcttgcagg catctcatga gtgtgtcttc ccgttttccg cctgaggtca ctgcgtggat
     6241 ggagcgctgg cgcctgctgc gcgacggcga gctgctcacc acccactcgc caagctggaa
     6301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgccgatcat aatcagccat
     6361 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     6421 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     6481 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6541 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaattc cctatagtga
     6601 gtcgtattaa attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6661 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6721 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6781 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6841 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7921 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8221 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8401 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8521 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8581 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8641 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8701 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8761 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8821 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8881 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8941 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     9001 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     9061 cgttctttaa gagtccacta ttaaagaacg tggactcttg ttccaaactg gaacaacact
     9121 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9181 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     9241 tacaattt
//
