LOCUS       sequence-495866-        2963 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Neuron-specific expression of WAChR-1m opsin fusion protein: 
            {WAChR-1m}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER
            tag}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Tose AJ, Nava AA, McGrath SN, Mardinly AR, Naka A
  TITLE     WAChRs are excitatory opsins sensitive to indoor lighting
  JOURNAL   bioRxiv 2025.09.12.675947; doi: 
            https://doi.org/10.1101/2025.09.12.675947
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.12.675947; doi: https://doi.org/10.1101/2025.09.12.675947"
COMMENT     Sequence Label: pAAV-Syn1>WAChR_1m-G-mScarlet-Soma-E; WPRE3-BG...
FEATURES             Location/Qualifiers
     source          1..2963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..27
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        49..496
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    496..520
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1529..2221
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWDRVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(1659..1677)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1927..1946
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2441..2465)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2526..2546)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(2743..2760)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2749..2956
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tctagacaac tttgtataga aaagttgctg cagagggccc tgcgtatgag tgcaagtggg
       61 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
      121 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
      181 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
      241 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
      301 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
      361 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
      421 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
      481 gtgcctgaga gcgcagcaag tttgtacaaa aaagcaggct gccaccatga gcgcctgtcc
      541 tacaccccct accgtggtgg cccccacaga tcaaagcccc aatcctaacg gaggcaccac
      601 ctcttgtgct caggctgccc cttctcctca gatgagcatg aattacaccc tgcttgcaac
      661 catcgacgga tttagcgccg tgattctgct tggaagcggc gtggtgttct ggtactactc
      721 ttggagggga accggctttg gagcccaccc ccatcccaag agaagacccc tggaactgaa
      781 taccctgtct gccgccatcg ccttcttaac cggcctgatg tacgtggcca agaccagcga
      841 ctttgctagg gacagcacca gcgagtttag cctgaacagg tactggtacc tggactacat
      901 cgtgacatgc cccctgatcg tgatggacct gtgcttcacc gtggacgtgc cctacaagct
      961 ggccttcacc ggcagcaccg ccgtgctgct ggtgatcgcc ctcgccagct tcatcgtgag
     1021 gagccccgag aagtacatct acttcgctat cggctgcacc ctgtttagca ttttcttcta
     1081 cttctttttc aacgaggtga gaaagaggat caggtacgtg cccgactgcg ccaagaagga
     1141 cctggagagg gccatgttca tcttcttcgg cttctggccc atgttccccg tgctgtggct
     1201 gctgggcttt cacaccaccg gcgtgatcag cgaggaggcc tttgctatca ttcacgcctt
     1261 cctagacatt acctgcaaga ccattttcgg cgtgttcatg ctgaggtgca gactgcacat
     1321 cgaggactac gcctgggatg agctgaaaag gcagagacag gagatcgacg aggagaatca
     1381 gaaaaccttg accgccgctg gcgccgatgg cgccaccgtg atgatgcccg ttgaagaaga
     1441 cgacccctac gctagcggag cggccgccaa gagcaggatc accagcgagg gcgagtacat
     1501 ccccctggac cagatcgata tcaacgtggt gagcaagggc gaggcagtga tcaaggagtt
     1561 catgcggttc aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg
     1621 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1681 tggccccctg cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc
     1741 cttcaccaag caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt
     1801 caagtgggac cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac
     1861 ctccctggag gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc
     1921 tgacggcccc gtaatgcaga agaagacaat gggctgggaa gcgtccaccg agcggttgta
     1981 ccccgaggac ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg
     2041 gcgctacctg gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg
     2101 cgcctacaac gtggaccgca agttggacat cacctcccac aacgaggact acaccgtggt
     2161 ggaacagtac gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     2221 gcagtcccag cccatcctca acaccaagga gatggccccg cagagcaagc ctccagagga
     2281 gctggagatg agcagcatgc ccagccccgt ggcccctctg cccgcacgca cggagggcgt
     2341 catcgacatg cggagcatgt ccagcattga cagcttcatc agctgtgcca cggacttccc
     2401 tgaagccacc agattcttct gctacgagaa cgaggtgtaa acccagcttt cttgtacaaa
     2461 gtgggaattc ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2521 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2581 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc
     2641 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2701 actgacaatt ccgtggtgga attcctagag ctcgctgatc agcctcgact gtgccttcta
     2761 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     2821 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     2881 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata
     2941 gcaggcatgc tggggagggc cgc
//
