LOCUS       sequence-495876-        8916 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for human GPR124 ECD fused to human VEGFR2 TMD and
            human FKBP1A F36V, N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: p3xFLAG-CMV-9-sGPR124-VEGFR2 TMD-FKBP
FEATURES             Location/Qualifiers
     source          1..8916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..19
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8..30
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     27..43
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..43
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    81..215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(105..124)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     159..178
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(867..1661)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(978..997)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1588..1607
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1715..2013)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     2011..2031
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    2055..2677
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     2459..2478
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     CDS             complement(2688..3008)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(5292..5357)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(5358..5402)
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     promoter        complement(5433..5636)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5462..5482)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5637..6016
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(6177..6194)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6177..6193)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6186..6208)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6471..6492)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6700..6719
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6833..6937
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6938..7006,7007..7798)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7156..7175)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7969..8557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8458..8477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8711..8728
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8845..8866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8881..8886,8887..8904,8905..8911)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttaat
       61 acgactcact atagggaatt gatccagaca tgataagata cattgatgag tttggacaaa
      121 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      181 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
      241 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
      301 gtggtatggc tgattatgat cggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      361 tacggttcca gcttggcgag tgggtggtga gcagctcgcc gtcgcgcagc aggcgccagc
      421 gctccatcca cgcagtgacc tcaggcggaa aacgggaaga cacactcatg agatgcctgc
      481 aagcaattcg ttctgtatca ggcgcaggag cgtcccgtcc gggtcgacca aagcggccat
      541 cgtgcctccc cactcctgca gttcgggggc atggatgcgc ggatagccgc tgctggtttc
      601 ctggatgccg acggatttgc actgccggta gaactccgcg aggtcgtcca gcctcaggca
      661 gcagctgaac caactcgcga ggggatcgag cccggggtgg gcgaagaact ccagcatgag
      721 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
      781 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
      841 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
      901 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
      961 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     1021 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     1081 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
     1141 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     1201 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     1261 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     1321 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     1381 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     1441 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     1501 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     1561 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     1621 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     1681 gtctcttgat cagatcccaa gctgatcaat tcctccaaaa aagcctcctc actacttctg
     1741 gaatagctca gaggccgagg cggcctcggc ctgcataaat aaaaaaaatt agtcagccat
     1801 ggggcggaga atgggcggaa ctgggcggag ttagcggagg cgggatgggc ggagttaggg
     1861 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     1921 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     1981 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagccaag
     2041 ctggccgcgt acccaattca acaggcatct actgagtgga cccaacgcat gagaggacag
     2101 tgccaagcaa gcaactcaaa tgtcccaccg gttgggcatg gccaggtagc ctatgctgtg
     2161 tctggacgtc ctcctgctgg tatagttatt ttaaaatcag aaggacaggg aagggagcag
     2221 tggttcacgc ctgtaatccc agcaatttgg gaggccaagg tgggtagatc acctgagatt
     2281 aggagttgga gaccagcctg gccaatatgg tgaaaccccg tctctaccaa aaaaacaaaa
     2341 attagctgag cctggtcatg catgcctgga atcccaacaa ctcgggaggc tgaggcagga
     2401 gaatcgcttg aacccaggag gcggagattg cagtgagcca agattgtgcc actgcactcc
     2461 agcttggttc ccaatagacc ccgcaggccc tacaggttgt cttcccaact tgccccttgc
     2521 tccataccac ccccctccac cccataatat tatagaagga cacctagtca gacaaaatga
     2581 tgcaacttaa ttttattagg acaaggctgg tgggcactgg agtggcaact tccagggcca
     2641 ggagaggcac tggggagggg tcacagggat gccacccggg atccttattc cagttttaga
     2701 agctccacat cgaagacgag agtggcatgt ggtgggatga tgcctgggtg cccagtggca
     2761 ccataggcat aatctggaga tatagtcagt ttggctctct gacccacact catctgggca
     2821 accccttctt cccagcctcg gatcacctcc tgcttgccta gcataaactt aaagggcttg
     2881 tttctgtccc gggaggaatc aactttcttt ccatcttcaa gcatcccggt gtagtgcacc
     2941 acgcaggtct ggccgcgctt ggggaaggtg cgcccgtctc ctggggagat agtctccacc
     3001 tgcactccct taacggtccg taggatgatg acaagaagta gccagaagaa catggcaatc
     3061 accgccgtgc ctactagaat aatgatttcc aaagaacccc ccccatccag ctccatgagc
     3121 acggccacat tgcccaagtg ctggcagtgc agggcgctga cattgggctg gctggagcgg
     3181 agctggcagc cctccgaggt ccagccccca gcctccccgg gcccctcctg gctccaccaa
     3241 gcggccacag gttcggctcc ctcagcccag tgccgcagcg aaacggccac tggctctgtc
     3301 aggtttccca cgccacagcc actggttcct gcgaagatga cgggggtggc cacgccacgc
     3361 ctcttgccag gcccagcagc tccagggcgg gaggtgttgc tgtggctgtg gaagaggcgg
     3421 ccatttcgga agacgagcag ttgcagggtg cagtctgggg gcaccggggg agccagggca
     3481 gccggaaggg atgagaatag actcgggggc agctggatgg aggccagggc cacgctgttc
     3541 ttgatgtgga aggacgacag agaaacattg ggcctcccgg tggtgcagcg gaagcggagc
     3601 tgctggtcag ctgggggctc gggctcaggt ggggggttct ggccagggct tcctggccgt
     3661 gtgcccggca cccctccctc cctcctctgg aaggctgtgc aggtcaggcc cacgtagctg
     3721 tgcggcttga tgaggtaggc ctccaatgcc acgttcctcg cattcactga gatgtgctgg
     3781 gcatgggggc tgagggcggc ccccccaatg cgctccaggg cacccacgat gcggctgcag
     3841 gccttgtcct cgcgctgggc cagccacagc aggtgctcgt ccaccagcat caggttgctg
     3901 gccatgtcca ccatcacctc taccagctct ttgatctggt cgacataacc caaaaatttc
     3961 tggatcatct gagccacata gactacatcc atcatgtctg aaaagctagc ggcctcggct
     4021 gtgtacacgc gcagctggtg agccagggtc agcgcattgg aggcattgat gggcatcagc
     4081 acgaaggtgt acagcaccct ggtgatgtcg ttggtgtaga gacagtggga gtagtcccct
     4141 ggctcccagc ggccggcacg gtcacaccgg cgggaggctc gggtgcccgg ggcacccccg
     4201 cccaggggca ctgaggtgaa gggatactgc aggcaggact ggtaggctgt gatgccagcc
     4261 agagttcggg gccacctgaa gtccccgcgg ttgttggcaa cacgctcggc ggggcagtag
     4321 gaggcagagg tctccagcac cacgatctcc accttcttgc tggcgttgcc ttgggccatg
     4381 gacacggtgc actcccactc gcctgaggcc cacacgccga tgtgagacag cgtcagctca
     4441 ctggtgatga aggtgcagtc gtggatgagg ctctcggcca ggaggatgcc cgcctgctca
     4501 tcaccctcca caggggctcg gttgtggtac cagcggatgc gggtgtcgtt gcccaggtag
     4561 ctggcagagc actggaaggg cagccgatcc ccctggaaca ccacttggcg tagggacggg
     4621 atgaggtggt gtgtgtgcag ctccagggcc ccctcgcagc agagctgggc ctcctggagg
     4681 ctgcccaggg cctgagcatg cagggcactg gggtaagcac agagcgtgtg ttccgacagc
     4741 tgcagggagc gattctgggc ccagggcagc agccagcgca ggtggcagtc acaggtcagg
     4801 aactcggtgc ccaagtccac aaccttaagg gctggcagct catcaaagac cccaggttgc
     4861 agactggaga agatgtttcc agatatgttt agtcggagaa gcctggggag gccctggaag
     4921 gtctcggagg tgagacagcc aatccggttg ttggagagat ctaaacgctt cagctccccc
     4981 aggcccagga aggcgcccgg ctgcactgtg ctgatgatgt tgttcctcag gtccagcttc
     5041 tccagcagtg acagtcccag gaaggagcca ttgcggagcc ccgtgatctt gttattgctc
     5101 aagagcaggg taacggtgcc gttaggcaga aggccgggct cgggaggctc cgggaggtcc
     5161 ccgccgctgc acaccaccct ccgccgagcc gggccaggga cgccgccgct cagccccttg
     5221 ggccgctccc ccgagcactt gcagctgcgg atggatagcg ggcagccggg cgctgaattc
     5281 gcggccgcaa gcttgtcatc gtcatccttg taatcgatgt catgatcttt ataatcaccg
     5341 tcatggtctt tgtagtcagc aactgcagct ccaacaagag ctaggatcag aagtgcagac
     5401 atggtgaatt aattctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt
     5461 acacgcctac cgcccatttg cgtcaacggg gcggggttat tacgacattt tggaaagtcc
     5521 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     5581 ccccgtgagt caaaccgcta tccacgccca ttggtgtact gccaaaaccg catcaccatg
     5641 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtcccg taaggtcatg
     5701 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcggactt
     5761 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     5821 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     5881 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     5941 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     6001 gtcaataatc aatgtcaaca tggcggtcat attggacatg agccaatata aatgtacata
     6061 ttatgatata gatacaacgt atgcaatggc caatagccaa tattgattta tgctatataa
     6121 ccaatgacta atatggctaa ttgccaatat tgattcaatg tatagatcag cttggcactg
     6181 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6241 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6301 tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa
     6361 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     6421 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6481 acaagagtcc acgttcttta atagtggact cttaaagaac gtggactcca acgtcaaagg
     6541 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     6601 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     6661 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     6721 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     6781 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     6841 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6901 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6961 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7021 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7081 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7141 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7201 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7261 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7321 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7381 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7441 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7501 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7561 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7621 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7681 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7741 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7801 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7861 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7921 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7981 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8041 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8101 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8161 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8221 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8281 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8341 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8401 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8461 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8521 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8581 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8641 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8701 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8761 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8821 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8881 tttacacttt atgcttccgg ctcgtatgtt gtgtgg
//
