LOCUS       sequence-495879-       10785 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for human GPR124, N-terminal 3xFLAG-SNAPf tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: p3xFLAG-CMV-9-SNAPf-GPR124
FEATURES             Location/Qualifiers
     source          1..10785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(53..121,122..913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(271..290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1084..1672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1573..1592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1826..1843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1960..1981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1996..2001,2002..2019,2020..2026)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2034..2050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2039..2061
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2058..2074
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2058..2074
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2088..2107
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2088..2106
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2112..2246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2136..2155)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2190..2209
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2898..3692)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3009..3028)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3619..3638
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3746..4044)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     4042..4062
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4086..4708
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     4490..4509
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     CDS             complement(8634..9179)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      9176..9185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9192..9257)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(9258..9302)
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     promoter        complement(9333..9536)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9362..9382)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9537..9916
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(10077..10094)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10077..10093)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10086..10108)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10371..10392)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10600..10619
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(10733..10785,1..52)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
       61 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      121 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      181 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      241 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      301 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      361 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      421 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      481 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      541 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      601 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      661 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      721 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      781 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      841 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      901 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
      961 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1201 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1741 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1801 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     1861 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1921 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1981 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     2041 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttaa tacgactcac
     2101 tatagggaat tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     2161 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2221 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2281 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     2341 ctgattatga tcggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2401 agcttggcga gtgggtggtg agcagctcgc cgtcgcgcag caggcgccag cgctccatcc
     2461 acgcagtgac ctcaggcgga aaacgggaag acacactcat gagatgcctg caagcaattc
     2521 gttctgtatc aggcgcagga gcgtcccgtc cgggtcgacc aaagcggcca tcgtgcctcc
     2581 ccactcctgc agttcggggg catggatgcg cggatagccg ctgctggttt cctggatgcc
     2641 gacggatttg cactgccggt agaactccgc gaggtcgtcc agcctcaggc agcagctgaa
     2701 ccaactcgcg aggggatcga gcccggggtg ggcgaagaac tccagcatga gatccccgcg
     2761 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     2821 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     2881 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     2941 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     3001 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     3061 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     3121 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
     3181 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     3241 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     3301 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     3361 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     3421 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     3481 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     3541 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     3601 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcagccgga
     3661 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     3721 tcagatccca agctgatcaa ttcctccaaa aaagcctcct cactacttct ggaatagctc
     3781 agaggccgag gcggcctcgg cctgcataaa taaaaaaaat tagtcagcca tggggcggag
     3841 aatgggcgga actgggcgga gttagcggag gcgggatggg cggagttagg ggcgggacta
     3901 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     3961 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     4021 gggagcctgg ggactttcca caccctaact gacacacatt ccacagccaa gctggccgcg
     4081 tacccaattc aacaggcatc tactgagtgg acccaacgca tgagaggaca gtgccaagca
     4141 agcaactcaa atgtcccacc ggttgggcat ggccaggtag cctatgctgt gtctggacgt
     4201 cctcctgctg gtatagttat tttaaaatca gaaggacagg gaagggagca gtggttcacg
     4261 cctgtaatcc cagcaatttg ggaggccaag gtgggtagat cacctgagat taggagttgg
     4321 agaccagcct ggccaatatg gtgaaacccc gtctctacca aaaaaacaaa aattagctga
     4381 gcctggtcat gcatgcctgg aatcccaaca actcgggagg ctgaggcagg agaatcgctt
     4441 gaacccagga ggcggagatt gcagtgagcc aagattgtgc cactgcactc cagcttggtt
     4501 cccaatagac cccgcaggcc ctacaggttg tcttcccaac ttgccccttg ctccatacca
     4561 cccccctcca ccccataata ttatagaagg acacctagtc agacaaaatg atgcaactta
     4621 attttattag gacaaggctg gtgggcactg gagtggcaac ttccagggcc aggagaggca
     4681 ctggggaggg gtcacaggga tgccacccgg gatccttaga cggtagtttc gctcttccag
     4741 agtccggtct tcatgcagcc gccgctggct acctccgcgc ccatcagggt gacgtcgtcg
     4801 tacttgcccc ccttgggggc gccgtttagg ctggcggcgt tgagcgggta cgagcggcga
     4861 tggctctcct tctccagcgc gccgccgccc ttgagactgt cgcggctggc gctgcggcgc
     4921 tggcctgccc ggcccgcctc agaaagcggc gcggcgctgg tgtcgctgcc ctcggacggc
     4981 gtgagcatgg gctcgccttc cagctgcagg ccggcgcccg ggctgttgcg gctgctgccc
     5041 aggtagctgt cggtggggct gttgtgcaga ctaccgctct cgctggacag cagctccagc
     5101 gcccccgccg cgcccccgcg cagggccttg agccggttct tgccgcaggc ctcccccgcg
     5161 cggtgtccct tggcccggct cttgtgcgcc cgacgcccgt ggtgcacgtt gttggggtgg
     5221 cggtgggcga ggtttccccg ggtgcccgcc ggctccggct ctccttcccc gccggccgcc
     5281 gcccccgcct cgcacacctg actctgggcc agctgcaggt tggtgagctt gcaggggccc
     5341 agagccagcg gatggccgct gctgccgctt ggggaggact tgagggaggg gggcccctcc
     5401 ccgaacaccg gggaaccgtc ctctgcggcg gcgggcaggg cccggggcgg ggcatggggg
     5461 gccgcgggag aggcaggggg gcagcaggcg cgccacgagg ctctcacgtc cctccgcctg
     5521 gcacagtggt gagtgaagac gaagaggccc agggcggagg ctgccacccc gtacaagcag
     5581 ctgcacacca cccggggcag ccagcgctgg gacactgcca gagccccgca ggcccacatg
     5641 gccaagtaca ggaagtgcgt ggtcaccagc gcccctagct ggactcccgg agaatagagg
     5701 ctgtcaccat cctccggggg cccgggcgtc cccactcgcg cgctcccagt agcaagaagg
     5761 gaacctgagt cactcaggag ggggccgctg cccctgagcc tggtggaacc cctcagctcc
     5821 tcccctgcct ccagggaggc cctgctgttg cccgccttgg ggttctgtgc cagaggaccc
     5881 cgtaagcgta gcccggcgca caggaaatag atccaggtga tgagcagaat caaagccaca
     5941 gggatgtaga aggcgccaag gcttggacgc cacaccagcc agcagtaggg gctgtggtcc
     6001 cggtagttgt ggatgttgac tgcagctgtg atgccacaga taatgagtgg aatccctcca
     6061 gcgatcaaat agaaccggag cataggactg ggagtaggca gagcggggtc cccttcttgc
     6121 ggagggggtg ccctccaggt gagctcctta tggagcactc gcgccttcac gcccatccag
     6181 agcagcgtgg atagggagga gtagtgcagg gtgatgccca ccgcctggca gaccatctgg
     6241 tagttggtga gtgtgatgcc ccccgcaaag acagcagagg tcatggctat gtggaagcac
     6301 aagttcagca gcatgtgcca gcctttccgg gacacacgga tggagctgtg gttgaggatg
     6361 taggtgatga tggtggcgaa gaggcagagc agcagcaagg ccgtgcaggg gtataccacg
     6421 gggtgcagcc ctgccccggc gccccccacc tccctgggaa aggcgctcag ctccatgagc
     6481 acggccacat tgcccaagtg ctggcagtgc agggcgctga cattgggctg gctggagcgg
     6541 agctggcagc cctccgaggt ccagccccca gcctccccgg gcccctcctg gctccaccaa
     6601 gcggccacag gttcggctcc ctcagcccag tgccgcagcg aaacggccac tggctctgtc
     6661 aggtttccca cgccacagcc actggttcct gcgaagatga cgggggtggc cacgccacgc
     6721 ctcttgccag gcccagcagc tccagggcgg gaggtgttgc tgtggctgtg gaagaggcgg
     6781 ccatttcgga agacgagcag ttgcagggtg cagtctgggg gcaccggggg agccagggca
     6841 gccggaaggg atgagaatag actcgggggc agctggatgg aggccagggc cacgctgttc
     6901 ttgatgtgga aggacgacag agaaacattg ggcctcccgg tggtgcagcg gaagcggagc
     6961 tgctggtcag ctgggggctc gggctcaggt ggggggttct ggccagggct tcctggccgt
     7021 gtgcccggca cccctccctc cctcctctgg aaggctgtgc aggtcaggcc cacgtagctg
     7081 tgcggcttga tgaggtaggc ctccaatgcc acgttcctcg cattcactga gatgtgctgg
     7141 gcatgggggc tgagggcggc ccccccaatg cgctccaggg cacccacgat gcggctgcag
     7201 gccttgtcct cgcgctgggc cagccacagc aggtgctcgt ccaccagcat caggttgctg
     7261 gccatgtcca ccatcacctc taccagctct ttgatctggt cgacataacc caaaaatttc
     7321 tggatcatct gagccacata gactacatcc atcatgtctg aaaagctagc ggcctcggct
     7381 gtgtacacgc gcagctggtg agccagggtc agcgcattgg aggcattgat gggcatcagc
     7441 acgaaggtgt acagcaccct ggtgatgtcg ttggtgtaga gacagtggga gtagtcccct
     7501 ggctcccagc ggccggcacg gtcacaccgg cgggaggctc gggtgcccgg ggcacccccg
     7561 cccaggggca ctgaggtgaa gggatactgc aggcaggact ggtaggctgt gatgccagcc
     7621 agagttcggg gccacctgaa gtccccgcgg ttgttggcaa cacgctcggc ggggcagtag
     7681 gaggcagagg tctccagcac cacgatctcc accttcttgc tggcgttgcc ttgggccatg
     7741 gacacggtgc actcccactc gcctgaggcc cacacgccga tgtgagacag cgtcagctca
     7801 ctggtgatga aggtgcagtc gtggatgagg ctctcggcca ggaggatgcc cgcctgctca
     7861 tcaccctcca caggggctcg gttgtggtac cagcggatgc gggtgtcgtt gcccaggtag
     7921 ctggcagagc actggaaggg cagccgatcc ccctggaaca ccacttggcg tagggacggg
     7981 atgaggtggt gtgtgtgcag ctccagggcc ccctcgcagc agagctgggc ctcctggagg
     8041 ctgcccaggg cctgagcatg cagggcactg gggtaagcac agagcgtgtg ttccgacagc
     8101 tgcagggagc gattctgggc ccagggcagc agccagcgca ggtggcagtc acaggtcagg
     8161 aactcggtgc ccaagtccac aaccttaagg gctggcagct catcaaagac cccaggttgc
     8221 agactggaga agatgtttcc agatatgttt agtcggagaa gcctggggag gccctggaag
     8281 gtctcggagg tgagacagcc aatccggttg ttggagagat ctaaacgctt cagctccccc
     8341 aggcccagga aggcgcccgg ctgcactgtg ctgatgatgt tgttcctcag gtccagcttc
     8401 tccagcagtg acagtcccag gaaggagcca ttgcggagcc ccgtgatctt gttattgctc
     8461 aagagcaggg taacggtgcc gttaggcaga aggccgggct cgggaggctc cgggaggtcc
     8521 ccgccgctgc acaccaccct ccgccgagcc gggccaggga cgccgccgct cagccccttg
     8581 ggccgctccc ccgagcactt gcagctgcgg atggatagcg ggcagccggg cgcacccagc
     8641 ccaggcttgc ccagtctgtg gccctcgtgg gccagcagcc actctttcac ggcgagcccg
     8701 ccctcgtagc cccccacgtc caggtcgccc tgcaccaccc ggtggcaggg gatcagaatg
     8761 ggcacgggat ttccgctcag ggcggttttc acggcggcgg tggcggcggg attgccggcc
     8821 agggcggcca ggtggctgta gctgatgacc tctccgaact tcaccacttt cagcagtttc
     8881 cacagcacct ggcgggtaaa gctctcctgc tggaacactg ggtggtgcag ggctggcaca
     8941 gggaactcct cgatggcctc aggctggtga aagtaggcgt tgagccaggc ggtggcctgc
     9001 atcagtggct ctggtccgcc cagcacggcg gctggggcag gcacttccac ggcgtcggcg
     9061 gcagatgttc ctttgcccag gaagatgata cggtgcaggc cctgttcgca cccagacagt
     9121 tccagcttgc ccagagggct atccagggtg gtgcgcttca tttcgcagtc tttgtccatg
     9181 gcggccgcaa gcttgtcatc gtcatccttg taatcgatgt catgatcttt ataatcaccg
     9241 tcatggtctt tgtagtcagc aactgcagct ccaacaagag ctaggatcag aagtgcagac
     9301 atggtgaatt aattctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt
     9361 acacgcctac cgcccatttg cgtcaacggg gcggggttat tacgacattt tggaaagtcc
     9421 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     9481 ccccgtgagt caaaccgcta tccacgccca ttggtgtact gccaaaaccg catcaccatg
     9541 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtcccg taaggtcatg
     9601 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcggactt
     9661 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     9721 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     9781 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     9841 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     9901 gtcaataatc aatgtcaaca tggcggtcat attggacatg agccaatata aatgtacata
     9961 ttatgatata gatacaacgt atgcaatggc caatagccaa tattgattta tgctatataa
    10021 ccaatgacta atatggctaa ttgccaatat tgattcaatg tatagatcag cttggcactg
    10081 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    10141 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    10201 tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa
    10261 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
    10321 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
    10381 acaagagtcc acgttcttta atagtggact cttaaagaac gtggactcca acgtcaaagg
    10441 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
    10501 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
    10561 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
    10621 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
    10681 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
    10741 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgct
//
