LOCUS       sequence-495883-        9794 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for human RECK, N-terminal 3xFLAG-SNAPf tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: p3xFLAG-CMV-9-SNAPf-RECK
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1902..2524
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2101..2120)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2548..2568)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2566..2864
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2918..3712
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2972..2991)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3582..3601
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4364..4498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4401..4420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4455..4474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4503..4522)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4504..4522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4536..4552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4536..4552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4549..4571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4560..4576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4584..4590,4591..4608,4609..4614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4629..4650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4767..4784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4938..5526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5018..5037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5697..6488,6489..6557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6320..6339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6558..6662)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6776..6795)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7003..7024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7287..7309
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7301..7318
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7302..7318
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        7479..7858
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7859..8062
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8013..8033
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             8093..8137
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             8138..8203
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      8210..8219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8216..8761
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 tcaggagttg taatgcacag tcagatcaag gagccatgaa tgacatgaag ttgtgggaga
       61 aaggaagcat aaagatgcca tttatcaata tacctgttct tgatattaaa aagtgccagc
      121 cagagatgtg gaaagcaata gcttgttcac tgcagattaa accttgtcat agtaaatctc
      181 ggggaagtat tatttgcaaa tcagattgtg tggagattct taaaaaatgt ggagaccaga
      241 acaaattccc tgaagaccac acagctgaaa gtatttgtga gcttctgtca cctacagatg
      301 atctgaagaa ttgtatacct ttggatacat acctcaggcc aagtacttta ggtaacattg
      361 tagaagaagt gactcatccc tgtaacccaa atccttgccc tgccaatgag ctctgtgaag
      421 taaaccgaaa aggatgtcca tctggagatc cctgtcttcc atacttttgt gttcaaggtt
      481 gcaaactggg agaagcttct gatttcattg tccgtcaagg gacactaatc caggtgccat
      541 catctgcagg ggaagttggt tgttataaaa tctgttcatg tggacaaagt ggactcttag
      601 aaaactgtat ggaaatgcac tgtatagacc tccagaagtc ttgtattgtt ggaggaaaaa
      661 gaaaaagtca tggaacatcc tttagtattg actgcaatgt ctgttcttgt tttgctggca
      721 atttggtgtg ctctacccgc ctttgcctca gtgagcacag ttcagaagat gaccgtcgta
      781 ccttcacagg tctgccctgt aactgtgcag atcagtttgt ccctgtatgt gggcagaatg
      841 ggcgcactta ccccagtgcc tgcattgctc gctgtgtggg cctccaagac catcagtttg
      901 agtttggatc atgcatgtca aaggatccat gtaatcctaa tccctgccaa aaaaaccaaa
      961 ggtgcatacc caaaccacag gtctgcctga cgacttttga taaatttgga tgtagccagt
     1021 atgagtgtgt accaagacag ctcgcgtgtg accaggtcca agatcctgtt tgtgacacag
     1081 accacatgga gcacaacaat ctctgcactt tataccaaag aggaaaaagc ctctcttaca
     1141 aaggtccctg ccagcccttt tgcagagcaa ccgagcccgt atgtgggcac aatggtgaga
     1201 cctacagcag tgtgtgtgct gcctactcgg atcgcgtggc agtcgattac tatggggact
     1261 gccaggccgt cggagtcctc tcagagcaca gctccgtcgc cgagtgtgct tctgtcaagt
     1321 gtccttcgct cttggcagct ggatgcaaac ccatcatccc accgggtgct tgttgcccat
     1381 tatgtgctgg gatgttaaga gttttatttg acaaagaaaa actggatact attgctaagg
     1441 taacaaataa aaagccaata acagttctgg aaatacttca gaaaatccgc atgcacgtgt
     1501 ctgtcccaca gtgtgatgtg tttggatact tcagcattga atcagaaatt gtgatcctga
     1561 tcattcccgt cgatcactat ccaaaagctc tgcagattga agcctgcaat aaagaagcag
     1621 agaagattga gtcccttatc aactctgaca gcccgacttt ggcgtcccat gtccctctct
     1681 ctgccctcat catttcccag gtacaggtct ccagcagtgt gccatcggcc ggtgtcaggg
     1741 ccaggccttc ttgccactcc ctcctccttc ccctcagctt gggccttgcc ttgcacttgc
     1801 tctggacata taactgactg cccacggaaa gtgcagaatg ctcctccacc tcactctcct
     1861 gccagatctg atatcggtac cagtcgactc tagaggatcc cgggtggcat ccctgtgacc
     1921 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
     1981 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
     2041 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
     2101 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
     2161 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
     2221 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
     2281 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     2341 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc
     2401 agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt
     2461 tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg
     2521 aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     2581 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     2641 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2701 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc tccgctaact ccgcccagtt
     2761 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcaggc cgaggccgcc
     2821 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggaat tgatcagctt
     2881 gggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     2941 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     3001 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     3061 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     3121 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     3181 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     3241 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     3301 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     3361 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     3421 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     3481 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     3541 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     3601 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     3661 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     3721 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     3781 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     3841 atcctccagc gcggggatct catgctggag ttcttcgccc accccgggct cgatcccctc
     3901 gcgagttggt tcagctgctg cctgaggctg gacgacctcg cggagttcta ccggcagtgc
     3961 aaatccgtcg gcatccagga aaccagcagc ggctatccgc gcatccatgc ccccgaactg
     4021 caggagtggg gaggcacgat ggccgctttg gtcgacccgg acgggacgct cctgcgcctg
     4081 atacagaacg aattgcttgc aggcatctca tgagtgtgtc ttcccgtttt ccgcctgagg
     4141 tcactgcgtg gatggagcgc tggcgcctgc tgcgcgacgg cgagctgctc accacccact
     4201 cgccaagctg gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccgat
     4261 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4321 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4381 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4441 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     4501 ttccctatag tgagtcgtat taaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     4561 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4621 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4681 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4741 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5521 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5581 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5641 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5701 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5761 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5821 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5881 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5941 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6001 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6061 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6121 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6181 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6241 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6301 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6361 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6421 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6481 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6541 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6601 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6661 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     6721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6901 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6961 acgttggagt ccacgttctt taagagtcca ctattaaaga acgtggactc ttgttccaaa
     7021 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7081 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7141 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     7201 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     7261 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     7321 caagctgatc tatacattga atcaatattg gcaattagcc atattagtca ttggttatat
     7381 agcataaatc aatattggct attggccatt gcatacgttg tatctatatc ataatatgta
     7441 catttatatt ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta
     7501 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     7561 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     7621 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     7681 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
     7741 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     7801 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     7861 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
     7921 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     7981 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
     8041 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag aattaattca ccatgtctgc
     8101 acttctgatc ctagctcttg ttggagctgc agttgctgac tacaaagacc atgacggtga
     8161 ttataaagat catgacatcg attacaagga tgacgatgac aagcttgcgg ccgccatgga
     8221 caaagactgc gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc
     8281 tgggtgcgaa cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga
     8341 cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc
     8401 caccgcctgg ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc
     8461 agccctgcac cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact
     8521 gctgaaagtg gtgaagttcg gagaggtcat cagctacagc cacctggccg ccctggccgg
     8581 caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct
     8641 gatcccctgc caccgggtgg tgcagggcga cctggacgtg gggggctacg agggcgggct
     8701 cgccgtgaaa gagtggctgc tggcccacga gggccacaga ctgggcaagc ctgggctggg
     8761 tgaggtggca gggggcctgg ctccgggcag tgcgggtgca ttgtgttgta atcattcaaa
     8821 ggataaccaa atgtgccgtg atgtatgtga acagattttc tcctcaaaaa gtgaatcccg
     8881 actaaaacat ctgttgcagc gagccccaga ttattgccca gagacaatgg ttgaaatttg
     8941 gaattgtatg aattcatctt tgccaggtgt gtttaagaag tctgatggct gggttggctt
     9001 aggctgctgt gaactggcta ttgccttgga gtgtcgacag gcatgcaagc aggcatcttc
     9061 aaagaatgat atttccaaag tttgcagaaa agaatatgag aatgctcttt tcagttgcat
     9121 tagcagaaat gaaatgggct cggtttgttg cagttatgca ggtcatcaca caaactgccg
     9181 agaatactgt caagccattt ttcgaacaga ctcttctcct ggtccatctc agataaaagc
     9241 agtggaaaat tattgcgcct ctattagtcc acaattaata cattgtgtga acaattatac
     9301 tcaatcttat ccaatgagga acccaacgga tagtttatat tgctgtgaca gagctgaaga
     9361 ccatgcttgc caaaatgcct gcaagagaat cctgatgtcc aagaaaacgg aaatggagat
     9421 tgttgatggt ctcatcgagg gttgtaagac ccagcccttg cctcaagatc ctctttggca
     9481 atgttttctt gaaagctcac aatctgttca ccctggagtc actgtacacc ctcctccctc
     9541 tacaggcctc gatggggcta aattgcattg ttgttctaaa gcaaacactt caacatgtag
     9601 ggaactctgc actaaacttt acagcatgag ctggggcaat acacagagtt ggcaagagtt
     9661 tgatcgcttt tgtgaatata atccagtgga agtgtccatg ttgacctgtt tagcggatgt
     9721 ccgggaacct tgccagttgg gctgtagaaa ccttacttac tgtactaatt ttaacaacag
     9781 gccaacagaa cttt
//
