LOCUS       sequence-495886-       10785 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for human GPR124, N-terminal 3xFLAG-CLIPf tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: p3xFLAG-CMV-9-CLIPf-GPR124
FEATURES             Location/Qualifiers
     source          1..10785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(84..152,153..944)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(302..321)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1115..1703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1604..1623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1857..1874
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1991..2012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2027..2032,2033..2050,2051..2057)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2065..2081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2070..2092
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2089..2105
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2089..2105
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2119..2138
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2119..2137
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2143..2277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2167..2186)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2221..2240
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2929..3723)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3040..3059)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3650..3669
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3777..4075)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     4073..4093
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4117..4739
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     4521..4540
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     CDS             complement(8665..9210)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylcytosine 
                     derivatives"
                     /label=CLIP-tag(TM)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      9207..9216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9223..9288)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(9289..9333)
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     promoter        complement(9364..9567)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9393..9413)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9568..9947
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(10108..10125)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10108..10124)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10117..10139)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10402..10423)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10631..10650
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(10764..10785,1..83)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
       61 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      121 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      181 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      241 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      301 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
      361 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      421 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      481 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      541 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      601 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
      661 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
      721 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
      781 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
      841 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
      901 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
      961 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1021 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1081 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1141 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1201 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1261 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1321 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1381 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1441 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1501 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1561 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1621 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1681 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1741 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1801 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     1861 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     1921 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     1981 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     2041 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     2101 atgacatgat tacgaattta atacgactca ctatagggaa ttgatccaga catgataaga
     2161 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2221 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2281 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2341 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcggcggag cctatggaaa
     2401 aacgccagca acgcggcctt tttacggttc cagcttggcg agtgggtggt gagcagctcg
     2461 ccgtcgcgca gcaggcgcca gcgctccatc cacgcagtga cctcaggcgg aaaacgggaa
     2521 gacacactca tgagatgcct gcaagcaatt cgttctgtat caggcgcagg agcgtcccgt
     2581 ccgggtcgac caaagcggcc atcgtgcctc cccactcctg cagttcgggg gcatggatgc
     2641 gcggatagcc gctgctggtt tcctggatgc cgacggattt gcactgccgg tagaactccg
     2701 cgaggtcgtc cagcctcagg cagcagctga accaactcgc gaggggatcg agcccggggt
     2761 gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga
     2821 aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg
     2881 gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc
     2941 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     3001 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     3061 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     3121 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     3181 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     3241 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     3301 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     3361 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     3421 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     3481 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     3541 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     3601 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3661 gccgaatagc ctctccaccc aagcagccgg agaacctgcg tgcaatccat cttgttcaat
     3721 catgcgaaac gatcctcatc ctgtctcttg atcagatccc aagctgatca attcctccaa
     3781 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctgcataa
     3841 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttagcgga
     3901 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     3961 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     4021 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     4081 tgacacacat tccacagcca agctggccgc gtacccaatt caacaggcat ctactgagtg
     4141 gacccaacgc atgagaggac agtgccaagc aagcaactca aatgtcccac cggttgggca
     4201 tggccaggta gcctatgctg tgtctggacg tcctcctgct ggtatagtta ttttaaaatc
     4261 agaaggacag ggaagggagc agtggttcac gcctgtaatc ccagcaattt gggaggccaa
     4321 ggtgggtaga tcacctgaga ttaggagttg gagaccagcc tggccaatat ggtgaaaccc
     4381 cgtctctacc aaaaaaacaa aaattagctg agcctggtca tgcatgcctg gaatcccaac
     4441 aactcgggag gctgaggcag gagaatcgct tgaacccagg aggcggagat tgcagtgagc
     4501 caagattgtg ccactgcact ccagcttggt tcccaataga ccccgcaggc cctacaggtt
     4561 gtcttcccaa cttgcccctt gctccatacc acccccctcc accccataat attatagaag
     4621 gacacctagt cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact
     4681 ggagtggcaa cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg
     4741 ggatccttag acggtagttt cgctcttcca gagtccggtc ttcatgcagc cgccgctggc
     4801 tacctccgcg cccatcaggg tgacgtcgtc gtacttgccc cccttggggg cgccgtttag
     4861 gctggcggcg ttgagcgggt acgagcggcg atggctctcc ttctccagcg cgccgccgcc
     4921 cttgagactg tcgcggctgg cgctgcggcg ctggcctgcc cggcccgcct cagaaagcgg
     4981 cgcggcgctg gtgtcgctgc cctcggacgg cgtgagcatg ggctcgcctt ccagctgcag
     5041 gccggcgccc gggctgttgc ggctgctgcc caggtagctg tcggtggggc tgttgtgcag
     5101 actaccgctc tcgctggaca gcagctccag cgcccccgcc gcgcccccgc gcagggcctt
     5161 gagccggttc ttgccgcagg cctcccccgc gcggtgtccc ttggcccggc tcttgtgcgc
     5221 ccgacgcccg tggtgcacgt tgttggggtg gcggtgggcg aggtttcccc gggtgcccgc
     5281 cggctccggc tctccttccc cgccggccgc cgcccccgcc tcgcacacct gactctgggc
     5341 cagctgcagg ttggtgagct tgcaggggcc cagagccagc ggatggccgc tgctgccgct
     5401 tggggaggac ttgagggagg ggggcccctc cccgaacacc ggggaaccgt cctctgcggc
     5461 ggcgggcagg gcccggggcg gggcatgggg ggccgcggga gaggcagggg ggcagcaggc
     5521 gcgccacgag gctctcacgt ccctccgcct ggcacagtgg tgagtgaaga cgaagaggcc
     5581 cagggcggag gctgccaccc cgtacaagca gctgcacacc acccggggca gccagcgctg
     5641 ggacactgcc agagccccgc aggcccacat ggccaagtac aggaagtgcg tggtcaccag
     5701 cgcccctagc tggactcccg gagaatagag gctgtcacca tcctccgggg gcccgggcgt
     5761 ccccactcgc gcgctcccag tagcaagaag ggaacctgag tcactcagga gggggccgct
     5821 gcccctgagc ctggtggaac ccctcagctc ctcccctgcc tccagggagg ccctgctgtt
     5881 gcccgccttg gggttctgtg ccagaggacc ccgtaagcgt agcccggcgc acaggaaata
     5941 gatccaggtg atgagcagaa tcaaagccac agggatgtag aaggcgccaa ggcttggacg
     6001 ccacaccagc cagcagtagg ggctgtggtc ccggtagttg tggatgttga ctgcagctgt
     6061 gatgccacag ataatgagtg gaatccctcc agcgatcaaa tagaaccgga gcataggact
     6121 gggagtaggc agagcggggt ccccttcttg cggagggggt gccctccagg tgagctcctt
     6181 atggagcact cgcgccttca cgcccatcca gagcagcgtg gatagggagg agtagtgcag
     6241 ggtgatgccc accgcctggc agaccatctg gtagttggtg agtgtgatgc cccccgcaaa
     6301 gacagcagag gtcatggcta tgtggaagca caagttcagc agcatgtgcc agcctttccg
     6361 ggacacacgg atggagctgt ggttgaggat gtaggtgatg atggtggcga agaggcagag
     6421 cagcagcaag gccgtgcagg ggtataccac ggggtgcagc cctgccccgg cgccccccac
     6481 ctccctggga aaggcgctca gctccatgag cacggccaca ttgcccaagt gctggcagtg
     6541 cagggcgctg acattgggct ggctggagcg gagctggcag ccctccgagg tccagccccc
     6601 agcctccccg ggcccctcct ggctccacca agcggccaca ggttcggctc cctcagccca
     6661 gtgccgcagc gaaacggcca ctggctctgt caggtttccc acgccacagc cactggttcc
     6721 tgcgaagatg acgggggtgg ccacgccacg cctcttgcca ggcccagcag ctccagggcg
     6781 ggaggtgttg ctgtggctgt ggaagaggcg gccatttcgg aagacgagca gttgcagggt
     6841 gcagtctggg ggcaccgggg gagccagggc agccggaagg gatgagaata gactcggggg
     6901 cagctggatg gaggccaggg ccacgctgtt cttgatgtgg aaggacgaca gagaaacatt
     6961 gggcctcccg gtggtgcagc ggaagcggag ctgctggtca gctgggggct cgggctcagg
     7021 tggggggttc tggccagggc ttcctggccg tgtgcccggc acccctccct ccctcctctg
     7081 gaaggctgtg caggtcaggc ccacgtagct gtgcggcttg atgaggtagg cctccaatgc
     7141 cacgttcctc gcattcactg agatgtgctg ggcatggggg ctgagggcgg cccccccaat
     7201 gcgctccagg gcacccacga tgcggctgca ggccttgtcc tcgcgctggg ccagccacag
     7261 caggtgctcg tccaccagca tcaggttgct ggccatgtcc accatcacct ctaccagctc
     7321 tttgatctgg tcgacataac ccaaaaattt ctggatcatc tgagccacat agactacatc
     7381 catcatgtct gaaaagctag cggcctcggc tgtgtacacg cgcagctggt gagccagggt
     7441 cagcgcattg gaggcattga tgggcatcag cacgaaggtg tacagcaccc tggtgatgtc
     7501 gttggtgtag agacagtggg agtagtcccc tggctcccag cggccggcac ggtcacaccg
     7561 gcgggaggct cgggtgcccg gggcaccccc gcccaggggc actgaggtga agggatactg
     7621 caggcaggac tggtaggctg tgatgccagc cagagttcgg ggccacctga agtccccgcg
     7681 gttgttggca acacgctcgg cggggcagta ggaggcagag gtctccagca ccacgatctc
     7741 caccttcttg ctggcgttgc cttgggccat ggacacggtg cactcccact cgcctgaggc
     7801 ccacacgccg atgtgagaca gcgtcagctc actggtgatg aaggtgcagt cgtggatgag
     7861 gctctcggcc aggaggatgc ccgcctgctc atcaccctcc acaggggctc ggttgtggta
     7921 ccagcggatg cgggtgtcgt tgcccaggta gctggcagag cactggaagg gcagccgatc
     7981 cccctggaac accacttggc gtagggacgg gatgaggtgg tgtgtgtgca gctccagggc
     8041 cccctcgcag cagagctggg cctcctggag gctgcccagg gcctgagcat gcagggcact
     8101 ggggtaagca cagagcgtgt gttccgacag ctgcagggag cgattctggg cccagggcag
     8161 cagccagcgc aggtggcagt cacaggtcag gaactcggtg cccaagtcca caaccttaag
     8221 ggctggcagc tcatcaaaga ccccaggttg cagactggag aagatgtttc cagatatgtt
     8281 tagtcggaga agcctgggga ggccctggaa ggtctcggag gtgagacagc caatccggtt
     8341 gttggagaga tctaaacgct tcagctcccc caggcccagg aaggcgcccg gctgcactgt
     8401 gctgatgatg ttgttcctca ggtccagctt ctccagcagt gacagtccca ggaaggagcc
     8461 attgcggagc cccgtgatct tgttattgct caagagcagg gtaacggtgc cgttaggcag
     8521 aaggccgggc tcgggaggct ccgggaggtc cccgccgctg cacaccaccc tccgccgagc
     8581 cgggccaggg acgccgccgc tcagcccctt gggccgctcc cccgagcact tgcagctgcg
     8641 gatggatagc gggcagccgg gcgcacccag cccaggcttg cccagtctgt ggccctcgtg
     8701 ggccagcagc cactctttca cggcgagccc gcccaggtag ggccccacgt cgctgtcgcc
     8761 ctgcaccacc cggtggcagg ggatcagaat gggcacggga tttccgtcca gggcggtgtt
     8821 cacggcggcg gtggcggcgg gattgcccac cagggcggcc aggtggctct cgctgatgac
     8881 ctctccgaac ttcaccactt tcagcagttt ccacagcacc tggcgggtaa agctctcctg
     8941 ctggaacact gggtggtgca gggctggcac agggaactcc tcgatggcct caggctggtg
     9001 aaagtaggcg ttgagccagg cggtggcctg gatcagtggc tctggtccgc ccagcacggc
     9061 ggctggggca ggcacttcca cggcgtcggc ggcagatgtt cctttgccca ggaagatgat
     9121 acggtgcagg ccctgttcgc acccagacag ttccagcttg cccagagggc tatccagggt
     9181 ggtgcgcttc atttcgcagt ctttgtccat ggcggccgca agcttgtcat cgtcatcctt
     9241 gtaatcgatg tcatgatctt tataatcacc gtcatggtct ttgtagtcag caactgcagc
     9301 tccaacaaga gctaggatca gaagtgcaga catggtgaat taattctgac ggttcactaa
     9361 acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaacgg
     9421 ggcggggtta ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     9481 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     9541 attggtgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
     9601 ctgccaagta ggaaagtccc gtaaggtcat gtactgggca taatgccagg cgggccattt
     9661 accgtcattg acgtcaatag ggggcggact tggcatatga tacacttgat gtactgccaa
     9721 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     9781 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     9841 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     9901 tgaccccgta attgattact attaataact agtcaataat caatgtcaac atggcggtca
     9961 tattggacat gagccaatat aaatgtacat attatgatat agatacaacg tatgcaatgg
    10021 ccaatagcca atattgattt atgctatata accaatgact aatatggcta attgccaata
    10081 ttgattcaat gtatagatca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
    10141 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    10201 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    10261 atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
    10321 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
    10381 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cacgttcttt aatagtggac
    10441 tcttaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    10501 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    10561 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    10621 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    10681 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
    10741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttat
//
