LOCUS       sequence-495887-        8900 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for a secreted human RECK-mouse IgG2a Fc fusion 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 8900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: pcDNA3.1-Ig-kappa SP-sRECK-Fc
FEATURES             Location/Qualifiers
     source          1..8900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      899..908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     905..964
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             3755..4450
                     /codon_start=1
                     /product="CH2 and CH3 domains of the mouse IgG2a heavy 
                     chain plus the hinge region"
                     /label=Fc tag
                     /translation="PRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVT
                     CVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF
                     KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIY
                     VEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHT
                     TKSFSRTPGK"
     primer_bind     complement(4491..4508)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4497..4721
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4767..5195
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4854..4873)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5064..5085
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5204..5224)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5209..5538
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5389..5524
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5451..5470
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5605..6399
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5659..5678)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6269..6288
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6573..6694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6610..6629)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6664..6683
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6743..6759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6743..6759)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6756..6778)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6767..6783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6791..6797,6798..6815,6816..6821))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6836..6857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6974..6991)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7145..7733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7225..7244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7904..8695,8696..8764))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8527..8546
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8765..8869)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
      961 tggtgacgcg gcccagccgg ccgaggtggc agggggcctg gctccgggca gtgcgggtgc
     1021 attgtgttgt aatcattcaa aggataacca aatgtgccgt gatgtatgtg aacagatttt
     1081 ctcctcaaaa agtgaatccc gactaaaaca tctgttgcag cgagccccag attattgccc
     1141 agagacaatg gttgaaattt ggaattgtat gaattcatct ttgccaggtg tgtttaagaa
     1201 gtctgatggc tgggttggct taggctgctg tgaactggct attgccttgg agtgtcgaca
     1261 ggcatgcaag caggcatctt caaagaatga tatttccaaa gtttgcagaa aagaatatga
     1321 gaatgctctt ttcagttgca ttagcagaaa tgaaatgggc tcggtttgtt gcagttatgc
     1381 aggtcatcac acaaactgcc gagaatactg tcaagccatt tttcgaacag actcttctcc
     1441 tggtccatct cagataaaag cagtggaaaa ttattgcgcc tctattagtc cacaattaat
     1501 acattgtgtg aacaattata ctcaatctta tccaatgagg aacccaacgg atagtttata
     1561 ttgctgtgac agagctgaag accatgcttg ccaaaatgcc tgcaagagaa tcctgatgtc
     1621 caagaaaacg gaaatggaga ttgttgatgg tctcatcgag ggttgtaaga cccagccctt
     1681 gcctcaagat cctctttggc aatgttttct tgaaagctca caatctgttc accctggagt
     1741 cactgtacac cctcctccct ctacaggcct cgatggggct aaattgcatt gttgttctaa
     1801 agcaaacact tcaacatgta gggaactctg cactaaactt tacagcatga gctggggcaa
     1861 tacacagagt tggcaagagt ttgatcgctt ttgtgaatat aatccagtgg aagtgtccat
     1921 gttgacctgt ttagcggatg tccgggaacc ttgccagttg ggctgtagaa accttactta
     1981 ctgtactaat tttaacaaca ggccaacaga acttttcagg agttgtaatg cacagtcaga
     2041 tcaaggagcc atgaatgaca tgaagttgtg ggagaaagga agcataaaga tgccatttat
     2101 caatatacct gttcttgata ttaaaaagtg ccagccagag atgtggaaag caatagcttg
     2161 ttcactgcag attaaacctt gtcatagtaa atctcgggga agtattattt gcaaatcaga
     2221 ttgtgtggag attcttaaaa aatgtggaga ccagaacaaa ttccctgaag accacacagc
     2281 tgaaagtatt tgtgagcttc tgtcacctac agatgatctg aagaattgta tacctttgga
     2341 tacatacctc aggccaagta ctttaggtaa cattgtagaa gaagtgactc atccctgtaa
     2401 cccaaatcct tgccctgcca atgagctctg tgaagtaaac cgaaaaggat gtccatctgg
     2461 agatccctgt cttccatact tttgtgttca aggttgcaaa ctgggagaag cttctgattt
     2521 cattgtccgt caagggacac taatccaggt gccatcatct gcaggggaag ttggttgtta
     2581 taaaatctgt tcatgtggac aaagtggact cttagaaaac tgtatggaaa tgcactgtat
     2641 agacctccag aagtcttgta ttgttggagg aaaaagaaaa agtcatggaa catcctttag
     2701 tattgactgc aatgtctgtt cttgttttgc tggcaatttg gtgtgctcta cccgcctttg
     2761 cctcagtgag cacagttcag aagatgaccg tcgtaccttc acaggtctgc cctgtaactg
     2821 tgcagatcag tttgtccctg tatgtgggca gaatgggcgc acttacccca gtgcctgcat
     2881 tgctcgctgt gtgggcctcc aagaccatca gtttgagttt ggatcatgca tgtcaaagga
     2941 tccatgtaat cctaatccct gccaaaaaaa ccaaaggtgc atacccaaac cacaggtctg
     3001 cctgacgact tttgataaat ttggatgtag ccagtatgag tgtgtaccaa gacagctcgc
     3061 gtgtgaccag gtccaagatc ctgtttgtga cacagaccac atggagcaca acaatctctg
     3121 cactttatac caaagaggaa aaagcctctc ttacaaaggt ccctgccagc ccttttgcag
     3181 agcaaccgag cccgtatgtg ggcacaatgg tgagacctac agcagtgtgt gtgctgccta
     3241 ctcggatcgc gtggcagtcg attactatgg ggactgccag gccgtcggag tcctctcaga
     3301 gcacagctcc gtcgccgagt gtgcttctgt caagtgtcct tcgctcttgg cagctggatg
     3361 caaacccatc atcccaccgg gtgcttgttg cccattatgt gctgggatgt taagagtttt
     3421 atttgacaaa gaaaaactgg atactattgc taaggtaaca aataaaaagc caataacagt
     3481 tctggaaata cttcagaaaa tccgcatgca cgtgtctgtc ccacagtgtg atgtgtttgg
     3541 atacttcagc attgaatcag aaattgtgat cctgatcatt cccgtcgatc actatccaaa
     3601 agctctgcag attgaagcct gcaataaaga agcagagaag attgagtccc ttatcaactc
     3661 tgacagcccg actttggcgt cccatgtccc tctctctgcc ctcatcattt cccaggtaca
     3721 ggtctccagc agtgtgccat cgactcgact agagcccaga gggcccacaa tcaagccctg
     3781 tcctccatgc aaatgcccag cacctaacct cttgggtgga ccatccgtct tcatcttccc
     3841 tccaaagatc aaggatgtac tcatgatctc cctgagcccc atagtcacat gtgtggtggt
     3901 ggatgtgagc gaggatgacc cagatgtcca gatcagctgg tttgtgaaca acgtggaagt
     3961 acacacagct cagacacaaa cccatagaga ggattacaac agtactctcc gggtggtcag
     4021 tgccctcccc atccagcacc aggactggat gagtggcaag gagttcaaat gcaaggtcaa
     4081 caacaaagac ctcccagcgc ccatcgagag aaccatctca aaacccaaag ggtcagtaag
     4141 agctccacag gtatatgtct tgcctccacc agaagaagag atgactaaga aacaggtcac
     4201 tctgacctgc atggtcacag acttcatgcc tgaagacatt tacgtggagt ggaccaacaa
     4261 cgggaaaaca gagctaaact acaagaacac tgaaccagtc ctggactctg atggttctta
     4321 cttcatgtac agcaagctga gagtggaaaa gaagaactgg gtggaaagaa atagctactc
     4381 ctgttcagtg gtccacgagg gtctgcacaa tcaccacacg actaagagct tctcccggac
     4441 tccgggtaaa tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     4501 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4561 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4621 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4681 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     4741 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     4801 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4861 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4921 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4981 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5041 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5101 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5161 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5221 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5281 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5341 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5401 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5461 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5521 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     5581 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5641 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5701 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5761 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5821 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5881 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5941 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6001 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6061 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6121 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6181 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6241 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6301 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6361 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6421 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6481 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6541 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     6601 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6661 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6721 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6781 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6841 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6901 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6961 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7021 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7081 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7141 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7201 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7261 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7321 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7381 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7441 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7501 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7561 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7621 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7681 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7741 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7801 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7861 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7921 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7981 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8041 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8101 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8161 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8221 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8281 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8341 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8401 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8461 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8521 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8581 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8641 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8701 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8761 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8821 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8881 gaaaagtgcc acctgacgtc
//
