LOCUS       sequence-495888-        9794 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expression vector for human RECK, N-terminal 3xFLAG-CLIPf tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, 
            Essler M, Noda M, Garcia KC, Kuo CJ
  TITLE     A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific 
            Regulation of Wnt Bioavailability.
  JOURNAL   Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 
            10.1016/j.celrep.2018.09.045.
  PUBMED    30304675
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-9- 9"; volume: "25"; issue: "2"; pages: "339-349.e9"
COMMENT     Sequence Label: p3xFLAG-CMV-9-CLIPf-RECK
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(752..1297)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylcytosine 
                     derivatives"
                     /label=CLIP-tag(TM)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      1294..1303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1310..1375)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(1376..1420)
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     promoter        complement(1451..1654)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1480..1500)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1655..2034
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2195..2212)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2195..2211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2204..2226)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2489..2510)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2718..2737
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2851..2955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2956..3024,3025..3816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3174..3193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3987..4575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4476..4495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4729..4746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4863..4884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4899..4904,4905..4922,4923..4929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4937..4953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4942..4964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4961..4977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4961..4977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4991..5010
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4991..5009
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5015..5149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5039..5058)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5093..5112
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5801..6595)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5912..5931)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6522..6541
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6649..6947)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     6945..6965
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    6989..7611
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     7393..7412
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 ggtgaacaga ttgtgagctt tcaagaaaac attgccaaag aggatcttga ggcaagggct
       61 gggtcttaca accctcgatg agaccatcaa caatctccat ttccgttttc ttggacatca
      121 ggattctctt gcaggcattt tggcaagcat ggtcttcagc tctgtcacag caatataaac
      181 tatccgttgg gttcctcatt ggataagatt gagtataatt gttcacacaa tgtattaatt
      241 gtggactaat agaggcgcaa taattttcca ctgcttttat ctgagatgga ccaggagaag
      301 agtctgttcg aaaaatggct tgacagtatt ctcggcagtt tgtgtgatga cctgcataac
      361 tgcaacaaac cgagcccatt tcatttctgc taatgcaact gaaaagagca ttctcatatt
      421 cttttctgca aactttggaa atatcattct ttgaagatgc ctgcttgcat gcctgtcgac
      481 actccaaggc aatagccagt tcacagcagc ctaagccaac ccagccatca gacttcttaa
      541 acacacctgg caaagatgaa ttcatacaat tccaaatttc aaccattgtc tctgggcaat
      601 aatctggggc tcgctgcaac agatgtttta gtcgggattc actttttgag gagaaaatct
      661 gttcacatac atcacggcac atttggttat cctttgaatg attacaacac aatgcacccg
      721 cactgcccgg agccaggccc cctgccacct cacccagccc aggcttgccc agtctgtggc
      781 cctcgtgggc cagcagccac tctttcacgg cgagcccgcc caggtagggc cccacgtcgc
      841 tgtcgccctg caccacccgg tggcagggga tcagaatggg cacgggattt ccgtccaggg
      901 cggtgttcac ggcggcggtg gcggcgggat tgcccaccag ggcggccagg tggctctcgc
      961 tgatgacctc tccgaacttc accactttca gcagtttcca cagcacctgg cgggtaaagc
     1021 tctcctgctg gaacactggg tggtgcaggg ctggcacagg gaactcctcg atggcctcag
     1081 gctggtgaaa gtaggcgttg agccaggcgg tggcctggat cagtggctct ggtccgccca
     1141 gcacggcggc tggggcaggc acttccacgg cgtcggcggc agatgttcct ttgcccagga
     1201 agatgatacg gtgcaggccc tgttcgcacc cagacagttc cagcttgccc agagggctat
     1261 ccagggtggt gcgcttcatt tcgcagtctt tgtccatggc ggccgcaagc ttgtcatcgt
     1321 catccttgta atcgatgtca tgatctttat aatcaccgtc atggtctttg tagtcagcaa
     1381 ctgcagctcc aacaagagct aggatcagaa gtgcagacat ggtgaattaa ttctgacggt
     1441 tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     1501 tcaacggggc ggggttatta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     1561 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     1621 cacgcccatt ggtgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     1681 agatgtactg ccaagtagga aagtcccgta aggtcatgta ctgggcataa tgccaggcgg
     1741 gccatttacc gtcattgacg tcaatagggg gcggacttgg catatgatac acttgatgta
     1801 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     1861 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     1921 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     1981 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacatg
     2041 gcggtcatat tggacatgag ccaatataaa tgtacatatt atgatataga tacaacgtat
     2101 gcaatggcca atagccaata ttgatttatg ctatataacc aatgactaat atggctaatt
     2161 gccaatattg attcaatgta tagatcagct tggcactggc cgtcgtttta caacgtcgtg
     2221 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     2281 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2341 atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2401 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2461 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac gttctttaat
     2521 agtggactct taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2581 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     2641 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2701 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2761 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2821 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2881 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2941 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3001 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3061 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3121 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3181 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3241 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3301 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3361 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3421 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3481 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3541 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3601 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3661 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3721 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3781 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3841 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3901 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3961 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4021 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4081 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4141 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4201 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4261 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4321 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4381 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4441 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4561 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4621 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4681 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4741 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4801 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4861 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     4921 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
     4981 attacgaatt taatacgact cactataggg aattgatcca gacatgataa gatacattga
     5041 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     5101 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     5161 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     5221 aaacctctac aaatgtggta tggctgatta tgatcggcgg agcctatgga aaaacgccag
     5281 caacgcggcc tttttacggt tccagcttgg cgagtgggtg gtgagcagct cgccgtcgcg
     5341 cagcaggcgc cagcgctcca tccacgcagt gacctcaggc ggaaaacggg aagacacact
     5401 catgagatgc ctgcaagcaa ttcgttctgt atcaggcgca ggagcgtccc gtccgggtcg
     5461 accaaagcgg ccatcgtgcc tccccactcc tgcagttcgg gggcatggat gcgcggatag
     5521 ccgctgctgg tttcctggat gccgacggat ttgcactgcc ggtagaactc cgcgaggtcg
     5581 tccagcctca ggcagcagct gaaccaactc gcgaggggat cgagcccggg gtgggcgaag
     5641 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     5701 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     5761 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     5821 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     5881 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     5941 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     6001 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     6061 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     6121 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     6181 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     6241 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     6301 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     6361 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     6421 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     6481 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     6541 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     6601 acgatcctca tcctgtctct tgatcagatc ccaagctgat caattcctcc aaaaaagcct
     6661 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctgcat aaataaaaaa
     6721 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttagcg gaggcgggat
     6781 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     6841 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     6901 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     6961 attccacagc caagctggcc gcgtacccaa ttcaacaggc atctactgag tggacccaac
     7021 gcatgagagg acagtgccaa gcaagcaact caaatgtccc accggttggg catggccagg
     7081 tagcctatgc tgtgtctgga cgtcctcctg ctggtatagt tattttaaaa tcagaaggac
     7141 agggaaggga gcagtggttc acgcctgtaa tcccagcaat ttgggaggcc aaggtgggta
     7201 gatcacctga gattaggagt tggagaccag cctggccaat atggtgaaac cccgtctcta
     7261 ccaaaaaaac aaaaattagc tgagcctggt catgcatgcc tggaatccca acaactcggg
     7321 aggctgaggc aggagaatcg cttgaaccca ggaggcggag attgcagtga gccaagattg
     7381 tgccactgca ctccagcttg gttcccaata gaccccgcag gccctacagg ttgtcttccc
     7441 aacttgcccc ttgctccata ccacccccct ccaccccata atattataga aggacaccta
     7501 gtcagacaaa atgatgcaac ttaattttat taggacaagg ctggtgggca ctggagtggc
     7561 aacttccagg gccaggagag gcactgggga ggggtcacag ggatgccacc cgggatcctc
     7621 tagagtcgac tggtaccgat atcagatctg gcaggagagt gaggtggagg agcattctgc
     7681 actttccgtg ggcagtcagt tatatgtcca gagcaagtgc aaggcaaggc ccaagctgag
     7741 gggaaggagg agggagtggc aagaaggcct ggccctgaca ccggccgatg gcacactgct
     7801 ggagacctgt acctgggaaa tgatgagggc agagagaggg acatgggacg ccaaagtcgg
     7861 gctgtcagag ttgataaggg actcaatctt ctctgcttct ttattgcagg cttcaatctg
     7921 cagagctttt ggatagtgat cgacgggaat gatcaggatc acaatttctg attcaatgct
     7981 gaagtatcca aacacatcac actgtgggac agacacgtgc atgcggattt tctgaagtat
     8041 ttccagaact gttattggct ttttatttgt taccttagca atagtatcca gtttttcttt
     8101 gtcaaataaa actcttaaca tcccagcaca taatgggcaa caagcacccg gtgggatgat
     8161 gggtttgcat ccagctgcca agagcgaagg acacttgaca gaagcacact cggcgacgga
     8221 gctgtgctct gagaggactc cgacggcctg gcagtcccca tagtaatcga ctgccacgcg
     8281 atccgagtag gcagcacaca cactgctgta ggtctcacca ttgtgcccac atacgggctc
     8341 ggttgctctg caaaagggct ggcagggacc tttgtaagag aggctttttc ctctttggta
     8401 taaagtgcag agattgttgt gctccatgtg gtctgtgtca caaacaggat cttggacctg
     8461 gtcacacgcg agctgtcttg gtacacactc atactggcta catccaaatt tatcaaaagt
     8521 cgtcaggcag acctgtggtt tgggtatgca cctttggttt ttttggcagg gattaggatt
     8581 acatggatcc tttgacatgc atgatccaaa ctcaaactga tggtcttgga ggcccacaca
     8641 gcgagcaatg caggcactgg ggtaagtgcg cccattctgc ccacatacag ggacaaactg
     8701 atctgcacag ttacagggca gacctgtgaa ggtacgacgg tcatcttctg aactgtgctc
     8761 actgaggcaa aggcgggtag agcacaccaa attgccagca aaacaagaac agacattgca
     8821 gtcaatacta aaggatgttc catgactttt tctttttcct ccaacaatac aagacttctg
     8881 gaggtctata cagtgcattt ccatacagtt ttctaagagt ccactttgtc cacatgaaca
     8941 gattttataa caaccaactt cccctgcaga tgatggcacc tggattagtg tcccttgacg
     9001 gacaatgaaa tcagaagctt ctcccagttt gcaaccttga acacaaaagt atggaagaca
     9061 gggatctcca gatggacatc cttttcggtt tacttcacag agctcattgg cagggcaagg
     9121 atttgggtta cagggatgag tcacttcttc tacaatgtta cctaaagtac ttggcctgag
     9181 gtatgtatcc aaaggtatac aattcttcag atcatctgta ggtgacagaa gctcacaaat
     9241 actttcagct gtgtggtctt cagggaattt gttctggtct ccacattttt taagaatctc
     9301 cacacaatct gatttgcaaa taatacttcc ccgagattta ctatgacaag gtttaatctg
     9361 cagtgaacaa gctattgctt tccacatctc tggctggcac tttttaatat caagaacagg
     9421 tatattgata aatggcatct ttatgcttcc tttctcccac aacttcatgt cattcatggc
     9481 tccttgatct gactgtgcat tacaactcct gaaaagttct gttggcctgt tgttaaaatt
     9541 agtacagtaa gtaaggtttc tacagcccaa ctggcaaggt tcccggacat ccgctaaaca
     9601 ggtcaacatg gacacttcca ctggattata ttcacaaaag cgatcaaact cttgccaact
     9661 ctgtgtattg ccccagctca tgctgtaaag tttagtgcag agttccctac atgttgaagt
     9721 gtttgcttta gaacaacaat gcaatttagc cccatcgagg cctgtagagg gaggagggtg
     9781 tacagtgact ccag
//
