LOCUS       sequence-495895-        9627 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expresses dox-inducible OptoCas13d or CasRx-QK634-LightR (LightR 
            domain inserted right after amino acid residue 634 of CasRx).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Zhu L, Nguyen LT, Bell AG, Gillmann KM, Oatman H, Hariri J, Myhrvold
            C, Toettcher JE
  TITLE     Multimodal control of Cas13 activity through domain insertion at an 
            allosteric hotspot
  JOURNAL   bioRxiv 2025.04.21.649818
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.04.21.649818"
COMMENT     Sequence Label: 
            LNPL503-piggybac-Dox-inducible-CasRx-QK634-LightR-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2378..2827
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLIQIMNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     CDS             3851..3871
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3900..4473
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3923..4475
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4067..4084)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4294..4313
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4475..5185
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4620..4638)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4888..4907
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5290..5878
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5343..5363)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5761..5772)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   6121..6183
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6295..6314)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6414..6436
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6474..6492)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6560..6664
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6665..6733,6734..7525)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6883..6902)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7696..8284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8185..8204
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8438..8455
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8572..8593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8608..8613,8614..8631,8632..8638)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8646..8662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8651..8673
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8670..8686
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8670..8686
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   8721..8755
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    9133..9151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9169..9187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9205..9223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9241..9259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9277..9295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9313..9331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9349..9367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9413..9437
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    9476..9523
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             9536..9556
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caaagtgtac atgacaacct tcgccgaagg cagcgacgcc aggctggaaa agatcgtgga
       61 gggcgacagc atcaggagcg tgaatgaggg cgaggccttc agcgctgaaa tggccgataa
      121 aaacgccggc tataagatcg gcaacgccaa attcagccat cctaagggct acgccgtggt
      181 ggctaacaac cctctgtata caggacccgt ccagcaggat atgctcggcc tgaaggaaac
      241 tctggaaaag aggtacttcg gcgagagcgc tgatggcaat gacaatattt gtatccaggt
      301 gatccataac atcctggaca ttgaaaaaat cctcgccgaa tacattacca acgccgccta
      361 cgccgtcaac aatatctccg gcctggataa ggacattatt ggattcggca agttctccac
      421 agtgtatacc tacgacgaat tcaaagaccc cgagcaccat agggccgctt tcaacaataa
      481 cgataagctc atcaacgcca tcaaggccca gtatgacgag ttcgacaact tcctcgataa
      541 ccccagactc ggctatttcg gccaggcctt tttcagcaag gagggcagaa attacatcat
      601 caattacggc aacgaatgct atgacattct ggccctcctg agcggactga ggcactgggt
      661 ggtccataac aacgaagaag agtccaggat ctccaggacc tggctctaca acctcgataa
      721 gaacctcgac aacgaataca tctccaccct caactacctc tacgacagga tcaccaatga
      781 gctgaccaac tccttctcca agaactccgc cgccaacgtg aactatattg ccgaaactct
      841 gggaatcaac cctgccgaat tcgccgaaca atatttcaga ttcagcatta tgaaagagca
      901 gaaaaacctc ggattcaata tcaccaagct cagggaagtg atgctggaca ggaaggatat
      961 gtccgagatc aggaaaaatc ataaggtgtt cgactccatc aggaccaagg tctacaccat
     1021 gatggacttt gtgatttata ggtattacat cgaagaggat gccaaggtgg ctgccgccaa
     1081 taagtccctc cccgataatg agaagtccct gagcgagaag gatatctttg tgattaacct
     1141 gaggggctcc ttcaacgacg accagaagga tgccctctac tacgatgaag ctaatagaat
     1201 ttggagaaag ctcgaaaata tcatgcacaa catcaaggaa tttaggggaa acaagacaag
     1261 agagtataag aagaaggacg cccctagact gcccagaatc ctgcccgctg gccgtgatgt
     1321 ttccgccttc agcaaactca tgtatgccct gaccatgttc ctggatggca aggagatcaa
     1381 cgacctcctg accaccctga ttaataaatt cgataacatc cagagcttcc tgaaggtgat
     1441 gcctctcatc ggagtcaacg ctaagttcgt ggaggaatac gcctttttca aagactccgc
     1501 caagatcgcc gatgagctga ggctgatcaa gtccttcgct agaatgggag aacctattgc
     1561 cgatgccagg agggccatgt atatcgacgc catccgtatt ttaggaacca acctgtccta
     1621 tgatgagctc aaggccctcg ccgacacctt ttccctggac gagaacggaa acaagctcaa
     1681 gaaaggcaag cacggcatga gaaatttcat tattaataac gtgatcagca ataaaaggtt
     1741 ccactacctg atcagatacg gtgatcctgc ccacctccat gagatcgcca aaaacgaggc
     1801 cgtggtgaag ttcgtgctcg gcaggatcgc tgacatccag ggaccaggtg gcagcggagg
     1861 tcataccttg tatgcgccgg ggggttatga catcatgggt tacctcatac agatcatgaa
     1921 taggccgaac ccacaagtgg agctcggacc cgtcgatacc tcctgcgctc tcattctgtg
     1981 tgaccttaag cagaaggata cccctatcgt gtacgcctcc gaggcatttc tgtacatgac
     2041 agggtactcg aacgccgaag tgctgggacg gaactgccgc ttcctgcaaa gcccggatgg
     2101 aatggtgaag cctaagtcaa cccgcaaata cgtggactcc aacactatca acaccatgcg
     2161 caaggccatt gaccgcaatg ctgaggtgca agtggaagtg gtgaacttca agaagaatgg
     2221 acagcgcttc gtcaacttcc tgactatgat ccccgtgcgc gacgagaccg gcgaataccg
     2281 gtacagcatg gggtttcagt gtgagacaga gggcggctcc ggaggcagcg gcggttctgg
     2341 aggttccggt ggcggctccg gaggtagcgg agggtctcac actctttacg cccctggagg
     2401 atacgacatt atgggatatt tgattcagat tatgaaccgc ccaaaccctc aggtcgaact
     2461 ggggcctgtg gacacgtcat gtgccctgat cctgtgcgat ctgaagcaaa aggacactcc
     2521 gattgtctac gcctcggaag ccttcttgta tatgaccgga tacagcaatg cagaggtgct
     2581 cggtaggaac tgcagattcc tgcagtcccc cgacgggatg gtgaaaccaa agtcgactcg
     2641 caaatatgtg gactcgaaca cgatcaatac aatgcggaag gccatcgacc ggaacgccga
     2701 ggtccaggtg gaggtggtca actttaagaa gaacggccag cggttcgtga actttctcac
     2761 catgattccg gtccgggatg aaaccggaga gtacagatac tccatgggat tccagtgcga
     2821 aaccgaaggg tccggaggtc ccggaaaaaa acagggccag aacggcaaga accagatcga
     2881 caggtactac gaaacttgta tcggaaagga taagggcaag agcgtgagcg aaaaggtgga
     2941 cgctctcaca aagatcatca ccggaatgaa ctacgaccaa ttcgacaaga aaaggagcgt
     3001 cattgaggac accggcaggg aaaacgccga gagggagaag tttaaaaaga tcatcagcct
     3061 gtacctcacc gtgatctacc acatcctcaa gaatattgtc aatatcaacg ccaggtacgt
     3121 catcggattc cattgcgtcg agcgtgatgc tcaactgtac aaggagaaag gctacgacat
     3181 caatctcaag aaactggaag agaagggatt cagctccgtc accaagctct gcgctggcat
     3241 tgatgaaact gcccccgata agagaaagga cgtggaaaag gagatggctg aaagagccaa
     3301 ggagagcatt gacagcctcg agagcgccaa ccccaagctg tatgccaatt acatcaaata
     3361 cagcgacgag aagaaagccg aggagttcac caggcagatt aacagggaga aggccaaaac
     3421 cgccctgaac gcctacctga ggaacaccaa gtggaatgtg atcatcaggg aggacctcct
     3481 gagaattgac aacaagacat gtaccctgtt cagaaacaag gccgtccacc tggaagtggc
     3541 caggtatgtc cacgcctata tcaacgacat tgccgaggtc aattcctact tccaactgta
     3601 ccattacatc atgcagagaa ttatcatgaa tgagaggtac gagaaaagca gcggaaaggt
     3661 gtccgagtac ttcgacgctg tgaatgacga gaagaagtac aacgataggc tcctgaaact
     3721 gctgtgtgtg cctttcggct actgtatccc caggtttaag aacctgagca tcgaggccct
     3781 gttcgatagg aacgaggccg ccaagttcga caaggagaaa aagaaggtgt ccggcaattc
     3841 cggatccgga cctaagaaaa agaggaaggt ggcggccgct taggtaccaa gctgatccgc
     3901 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     3961 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4021 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4081 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4141 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     4201 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     4261 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     4321 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac
     4381 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     4441 gacgtggttt tcctttgaaa aacacgatga taatatggtg agcaagggcg aggaggataa
     4501 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     4561 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     4621 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     4681 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     4741 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4801 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     4861 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     4921 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     4981 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     5041 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     5101 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     5161 cggcatggac gagctgtaca agtagcctgc aggcggccgc aactcccacc tgcaacatgc
     5221 gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     5281 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5341 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5401 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5461 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5521 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5581 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5641 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5701 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5761 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5821 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     5881 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgata
     5941 ctagttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     6001 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     6061 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     6121 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     6181 gggttaatct agtatacgcg tatgcggccg cttaattaat ccatggcgcc tgatgcggta
     6241 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     6301 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     6361 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6421 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     6481 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6901 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7201 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7381 gtcccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7801 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7981 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8041 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8101 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8161 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8221 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8281 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8341 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8401 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8461 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8521 ctggcacgac aggtttcccg actggaaagc gggcagtgat cgcaacgcaa ttaatgtgag
     8581 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     8641 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccgg
     8701 ggcgcgccat tctagattaa ccctagaaag atagtctgcg taaaattgac gcatgcattc
     8761 ttgaaatatt gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc
     8821 tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt
     8881 ttgaactata acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta
     8941 agatttaact catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat
     9001 tatatatata ttttcttgtt atagatatca actagaatgc tagcatgggc ccagatctcc
     9061 gaggggaccc gacaggcccg cagggacagc agagatccag tttggatcga taagcttgat
     9121 atcgaatttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     9181 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     9241 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     9301 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     9361 atagagaacg tatgtcgagg taggcgtgta cggtgggcgc ctataaaagc agagctcgtt
     9421 tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat acttgggttt
     9481 gtctggtcaa ccaccgcggt ctcagtggtg tacggtacaa accgccacca tgagccccaa
     9541 gaagaagaga aaggtggagg ccagcatcga aaaaaaaaag tccttcgcca agggcatggg
     9601 cgtgaagtcc acactcgtgt ccggctc
//
