LOCUS       sequence-495896-        9234 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  Expresses dox-inducible ChemoCas13d or CasRx-QK634-LightR (UnirapR 
            domain inserted right after amino acid residue 634 of CasRx).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Zhu L, Nguyen LT, Bell AG, Gillmann KM, Oatman H, Hariri J, Myhrvold
            C, Toettcher JE
  TITLE     Multimodal control of Cas13 activity through domain insertion at an 
            allosteric hotspot
  JOURNAL   bioRxiv 2025.04.21.649818
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.04.21.649818"
COMMENT     Sequence Label: 
            LNPL1093-piggybac-Dox-inducible-CasRx-QK634-UnirapR-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..30
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    59..632
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    82..634
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(226..243)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     453..472
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             634..1344
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(779..797)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1047..1066
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1449..2037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1502..1522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1920..1931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   2280..2342
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2454..2473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2573..2595
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2633..2651)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2719..2823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2824..2892,2893..3684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3042..3061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3855..4443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4344..4363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4597..4614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4731..4752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4767..4772,4773..4790,4791..4797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4805..4821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4810..4832
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4829..4845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4829..4845
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   4880..4914
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    5292..5310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5328..5346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5364..5382
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5400..5418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5436..5454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5472..5490
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5508..5526
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5572..5596
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5635..5682
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             5695..5715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggatccggac ctaagaaaaa gaggaaggtg gcggccgctt aggtaccaag ctgatccgcc
       61 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
      121 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
      181 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
      241 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
      301 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
      361 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
      421 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
      481 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca
      541 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
      601 acgtggtttt cctttgaaaa acacgatgat aatatggtga gcaagggcga ggaggataac
      661 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      721 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      781 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      841 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      901 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      961 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     1021 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1081 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     1141 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     1201 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     1261 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     1321 ggcatggacg agctgtacaa gtagcctgca ggcggccgca actcccacct gcaacatgcg
     1381 tgactgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
     1441 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     1501 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     1561 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     1621 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     1681 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     1741 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     1801 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     1861 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     1921 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     1981 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     2041 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgatac
     2101 tagttaaaag ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa
     2161 taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa
     2221 taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc gataaaacac
     2281 atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag
     2341 ggttaatcta gtatacgcgt atgcggccgc ttaattaatc catggcgcct gatgcggtat
     2401 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     2461 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     2521 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     2581 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     2641 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     2701 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2761 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2821 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2881 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2941 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     3001 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     3061 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3121 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3181 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3241 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3301 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3361 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3421 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3481 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3541 tcccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3601 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3661 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3721 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3781 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3841 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3901 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3961 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4021 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4081 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4141 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4201 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4261 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4321 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4381 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4441 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4501 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4561 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4621 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4681 tggcacgaca ggtttcccga ctggaaagcg ggcagtgatc gcaacgcaat taatgtgagt
     4741 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     4801 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccggg
     4861 gcgcgccatt ctagattaac cctagaaaga tagtctgcgt aaaattgacg catgcattct
     4921 tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct
     4981 cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt
     5041 tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa
     5101 gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt
     5161 atatatatat tttcttgtta tagatatcaa ctagaatgct agcatgggcc cagatctccg
     5221 aggggacccg acaggcccgc agggacagca gagatccagt ttggatcgat aagcttgata
     5281 tcgaatttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
     5341 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
     5401 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
     5461 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
     5521 tagagaacgt atgtcgaggt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt
     5581 agtgaaccgt cagatcgcct ggagcaattc cacaacactt ttgtcttata cttgggtttg
     5641 tctggtcaac caccgcggtc tcagtggtgt acggtacaaa ccgccaccat gagccccaag
     5701 aagaagagaa aggtggaggc cagcatcgaa aaaaaaaagt ccttcgccaa gggcatgggc
     5761 gtgaagtcca cactcgtgtc cggctccaaa gtgtacatga caaccttcgc cgaaggcagc
     5821 gacgccaggc tggaaaagat cgtggagggc gacagcatca ggagcgtgaa tgagggcgag
     5881 gccttcagcg ctgaaatggc cgataaaaac gccggctata agatcggcaa cgccaaattc
     5941 agccatccta agggctacgc cgtggtggct aacaaccctc tgtatacagg acccgtccag
     6001 caggatatgc tcggcctgaa ggaaactctg gaaaagaggt acttcggcga gagcgctgat
     6061 ggcaatgaca atatttgtat ccaggtgatc cataacatcc tggacattga aaaaatcctc
     6121 gccgaataca ttaccaacgc cgcctacgcc gtcaacaata tctccggcct ggataaggac
     6181 attattggat tcggcaagtt ctccacagtg tatacctacg acgaattcaa agaccccgag
     6241 caccataggg ccgctttcaa caataacgat aagctcatca acgccatcaa ggcccagtat
     6301 gacgagttcg acaacttcct cgataacccc agactcggct atttcggcca ggcctttttc
     6361 agcaaggagg gcagaaatta catcatcaat tacggcaacg aatgctatga cattctggcc
     6421 ctcctgagcg gactgaggca ctgggtggtc cataacaacg aagaagagtc caggatctcc
     6481 aggacctggc tctacaacct cgataagaac ctcgacaacg aatacatctc caccctcaac
     6541 tacctctacg acaggatcac caatgagctg accaactcct tctccaagaa ctccgccgcc
     6601 aacgtgaact atattgccga aactctggga atcaaccctg ccgaattcgc cgaacaatat
     6661 ttcagattca gcattatgaa agagcagaaa aacctcggat tcaatatcac caagctcagg
     6721 gaagtgatgc tggacaggaa ggatatgtcc gagatcagga aaaatcataa ggtgttcgac
     6781 tccatcagga ccaaggtcta caccatgatg gactttgtga tttataggta ttacatcgaa
     6841 gaggatgcca aggtggctgc cgccaataag tccctccccg ataatgagaa gtccctgagc
     6901 gagaaggata tctttgtgat taacctgagg ggctccttca acgacgacca gaaggatgcc
     6961 ctctactacg atgaagctaa tagaatttgg agaaagctcg aaaatatcat gcacaacatc
     7021 aaggaattta ggggaaacaa gacaagagag tataagaaga aggacgcccc tagactgccc
     7081 agaatcctgc ccgctggccg tgatgtttcc gccttcagca aactcatgta tgccctgacc
     7141 atgttcctgg atggcaagga gatcaacgac ctcctgacca ccctgattaa taaattcgat
     7201 aacatccaga gcttcctgaa ggtgatgcct ctcatcggag tcaacgctaa gttcgtggag
     7261 gaatacgcct ttttcaaaga ctccgccaag atcgccgatg agctgaggct gatcaagtcc
     7321 ttcgctagaa tgggagaacc tattgccgat gccaggaggg ccatgtatat cgacgccatc
     7381 cgtattttag gaaccaacct gtcctatgat gagctcaagg ccctcgccga caccttttcc
     7441 ctggacgaga acggaaacaa gctcaagaaa ggcaagcacg gcatgagaaa tttcattatt
     7501 aataacgtga tcagcaataa aaggttccac tacctgatca gatacggtga tcctgcccac
     7561 ctccatgaga tcgccaaaaa cgaggccgtg gtgaagttcg tgctcggcag gatcgctgac
     7621 atccagggac caggtacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa
     7681 tttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg
     7741 atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact
     7801 atatctccag attatgccta tggtgccact gggcacggtt cgggctccgg atcaggcgtc
     7861 aaggacctcc tccaagcctg ggacctctat tatcatgtgt tccgacgaat ctcaggtcct
     7921 ccaggacctg gatcaggtct ctggcatgag atgtggcatg aaggcctgga agaggcatct
     7981 cgtttgtact ttggggaaag gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat
     8041 gctatgatgg aacggggccc ccagactctg aaggaaacat cctttaatca ggcctatggt
     8101 cgagatttaa tggaggccca agagtggtgc aggaagtaca tgaaatcagg gtcatcaggg
     8161 ggctccggat caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
     8221 aaactggaag gtcccggaaa aaaacagggc cagaacggca agaaccagat cgacaggtac
     8281 tacgaaactt gtatcggaaa ggataagggc aagagcgtga gcgaaaaggt ggacgctctc
     8341 acaaagatca tcaccggaat gaactacgac caattcgaca agaaaaggag cgtcattgag
     8401 gacaccggca gggaaaacgc cgagagggag aagtttaaaa agatcatcag cctgtacctc
     8461 accgtgatct accacatcct caagaatatt gtcaatatca acgccaggta cgtcatcgga
     8521 ttccattgcg tcgagcgtga tgctcaactg tacaaggaga aaggctacga catcaatctc
     8581 aagaaactgg aagagaaggg attcagctcc gtcaccaagc tctgcgctgg cattgatgaa
     8641 actgcccccg ataagagaaa ggacgtggaa aaggagatgg ctgaaagagc caaggagagc
     8701 attgacagcc tcgagagcgc caaccccaag ctgtatgcca attacatcaa atacagcgac
     8761 gagaagaaag ccgaggagtt caccaggcag attaacaggg agaaggccaa aaccgccctg
     8821 aacgcctacc tgaggaacac caagtggaat gtgatcatca gggaggacct cctgagaatt
     8881 gacaacaaga catgtaccct gttcagaaac aaggccgtcc acctggaagt ggccaggtat
     8941 gtccacgcct atatcaacga cattgccgag gtcaattcct acttccaact gtaccattac
     9001 atcatgcaga gaattatcat gaatgagagg tacgagaaaa gcagcggaaa ggtgtccgag
     9061 tacttcgacg ctgtgaatga cgagaagaag tacaacgata ggctcctgaa actgctgtgt
     9121 gtgcctttcg gctactgtat ccccaggttt aagaacctga gcatcgaggc cctgttcgat
     9181 aggaacgagg ccgccaagtt cgacaaggag aaaaagaagg tgtccggcaa ttcc
//
