LOCUS       sequence-495900-       14534 bp    DNA     circular SYN 19-DEC-2025
DEFINITION  AMA1 based vector for optimised episomal expression in filamentous 
            fungi, with increased selection stringency using the modified 
            ubi-Y-pyrG marker (high stringency)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14534)
  AUTHORS   Roux I, Woodcraft C, Sbaraini N, Pepper A, Wong E, Bracegirdle J, 
            Chooi YH
  TITLE     Next-generation AMA1-based plasmids for enhanced heterologous 
            expression in filamentous fungi.
  JOURNAL   Microb Biotechnol. 2024 Sep;17(9):e70010. doi: 
            10.1111/1751-7915.70010.
  PUBMED    39276061
REFERENCE   2  (bases 1 to 14534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol."; date: "2024-09"; volume: "17(9):e70010. doi"; pages: "
            10.1111/1751-7915.70010"
COMMENT     Sequence Label: pYFAC-ubi-Y-pyrG
FEATURES             Location/Qualifiers
     source          1..14534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      1008..2350
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(1971..2018)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     2391..2409
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2447..2469)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2569..2588
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2612..2827
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2828..3631
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(3913..3930)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3913..3929)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3922..3944)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4229..4248)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4270..9518
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     primer_bind     9688..9709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             11269..11496
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             join(13504..13572,13573..14364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13722..13741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccactgaac gaagcatctg
     1021 tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     1081 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa
     1141 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     1201 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
     1261 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
     1321 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
     1381 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc
     1441 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
     1501 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
     1561 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
     1621 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
     1681 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
     1741 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
     1801 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
     1861 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
     1921 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
     1981 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg
     2041 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat
     2101 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt
     2161 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg
     2221 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt
     2281 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg
     2341 atattggatc atactaagaa accattatta tcatgacatt aacctataaa aataggcgta
     2401 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     2461 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     2521 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     2581 agattgtact gagagtgcac cataccacct tttcaattca tcattttttt tttattcttt
     2641 tttttgattt cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga
     2701 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
     2761 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
     2821 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
     2881 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
     2941 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
     3001 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
     3061 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg
     3121 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat
     3181 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga
     3241 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
     3301 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
     3361 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
     3421 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag
     3481 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt
     3541 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
     3601 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
     3661 actaaactca caaattagag cttcaattta attatatcag ttattaccct atgcggtgtg
     3721 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     3781 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3841 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtggggtacc
     3901 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3961 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4021 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     4081 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     4141 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4201 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4261 acgttcgcca gcttattttt tgtatactgt tttgtgatag cacgaagttt ttccacggta
     4321 tcttgttaaa aatatatatt tgtggcgggc ttacctacat caaattaata agagactaat
     4381 tataaactaa acacacaagc aagctacttt agggtaaaag tttataaatg cttttgacgt
     4441 ataaacgttg cttgtattta ttattacaat taaaggtgga tagaaaacct agagactagt
     4501 tagaaactaa tctcaggttt gcgttaaact aaatcagagc ccgagaggtt aacagaacct
     4561 agaaggggac tagatatccg ggtagggaaa caaaaaaaaa aaacaagaca gccacatatt
     4621 agggagacta gttagaagct agttccagga ctaggaaaat aaaagacaat gataccacag
     4681 tctagttgac aactagatag attctagatt gaggccaaag tctctgagat ccaggttagt
     4741 tgcaactaat actagttagt atctagtctc ctataactct gaagctagaa taacttacta
     4801 ctattatcct caccactgtt cagctgcgca aacggagtga ttgcaaggtg ttcagagact
     4861 agttattgac tagtcagtga ctagcaataa ctaacaaggt attaacctac catgtctgcc
     4921 atcaccctgc acttcctcgg gctcagcagc cttttcctcc tcattttcat gctcattttc
     4981 cttgtttaag actgtgacta gtcaaagact agtccagaac cacaaaggag aaatgtctta
     5041 ccactttctt cattgcttgt ctcttttgca ttatccatgt ctgcaactag ttagagtcta
     5101 gttagtgact agtccgacga ggacttgctt gtctccggat tgttggagga actctccagg
     5161 gcctcaagat ccacaacaga gccttctaga agactggtca ataactagtt ggtctttgtc
     5221 tgagtctgac ttacgaggtt gcatactcgc tccctttgcc tcgtcaatcg atgagaaaaa
     5281 gcgccaaaac tcgcaatatg gctttgaacc acacggtgct gagactagtt agaatctagt
     5341 cccaaactag cttggatagc ttacctttgc cctttgcgtt gcgacaggtc ttgcagggta
     5401 tggttccttt ctcaccagct gatttagctg ccttgctacc ctcacggcgg atctgcataa
     5461 agagtggcta gaggttataa attagcactg atcctaggta cggggctgaa tgtaacttgc
     5521 ctttcctttc tcatcgcgcg gcaagacagg cttgctcaaa ttcctaccag tcacaggggt
     5581 atgcacggcg tacggaccac ttgaactagt cacagattag ttagcaacta gtctgcattg
     5641 aatggctgta cttacgggcc ctcgccattg tcctgatcat ttccagcttc accctcgttg
     5701 ctgcaaagta gttagtgact agtcaaggac tagttgaaat gggagaagaa actcacgaat
     5761 tctcgacacc cttagtattg tggtccttgg acttggtgct gctatatatt agctaataca
     5821 ctagttagac tcacagaaac ttacgcagct cgcttgcgct tcttggtagg agtcggggtt
     5881 gggagaacag tgccttcaaa caagccttca taccatgcta cttgactagt cagggactag
     5941 tcaccaagta atctagatag gacttgcctt tggcctccat cagttccttc atagtgggag
     6001 gtccattgtg caatgtaaac tccatgccgt gggagttctt gtccttcaag tgcttgacca
     6061 atatgtttct gttggcagag ggaacctgtc aactagttaa taactagtca gaaactagta
     6121 tagcagtaga ctcactgtac gcttgaggca tcccttcact cggcagtaga cttcatatgg
     6181 atggatatca ggcacgccat tgtcgtcctg tggactagtc agtaactagg cttaaagcta
     6241 gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc tccccgtcct taactgcctc
     6301 gagatagtga cagtactctg gggactttcg gagatcgtta tcgcgaatgc tcggcatact
     6361 aatcgttgac tagtcttgga ctagtcccga gcaaaaagga ttggaggagg aggaggaagg
     6421 tgagagtgag acaaagagcg aaataagagc ttcaaaggct atctctaagc agtatgaagg
     6481 ttaagtatct agttcttgac tagatttaaa agagatttcg actagttatg tacctggagt
     6541 ttggatatag gaatgtgttg tggtaacgaa atgtaagggg gaggaaagaa aaagtcggtc
     6601 aagaggtaac tctaagtcgg ccattccttt ttgggaggcg ctaaccataa acggcatggt
     6661 cgacttagag ttagctcagg gaatttaggg agttatctgc gaccactgga ctctcaggct
     6721 ttccattggg gtactaacat agccatcaaa tgccggcccg tgtttaatct cctcgagatg
     6781 gctgggctca catgcgttct actcaaaaac tcttatacct aggccctctc ccaatcgtgt
     6841 atgtttggac cttcctctct cacactttgg ataacatcaa gcatactgca gattttgacc
     6901 tagttaacaa ggcacatcat gctatcactt ccaacactct tgctgagcgc gttctgcctt
     6961 gcctttctgg ttctacgaag tatccagtcc cgcctgcaac gccgcagaaa cgccaaaagg
     7021 tggggttgtc aaccgccagc tagagctcca tcgggattct ttggcattcc gccgttcctc
     7081 ggtggtcgca gataactccc taaattccct gagctaactc taagtcgacc atgccgttta
     7141 tggttagcgc ctcccaaaaa ggaatggccg acttagagtt acctcttgac cgactttttc
     7201 tttcctcccc cttacatttc gttaccacaa cacattccta tatccaaact ccaggtacat
     7261 aactagtcga aatctctttt aaatctagtc aagaactaga tacttaacct tcatactgct
     7321 tagagatagc ctttgaagct cttatttcgc tctttgtctc actctcacct tcctcctcct
     7381 cctccaatcc tttttgctcg ggactagtcc aagactagtc aacgattagt atgccgagca
     7441 ttcgcgataa cgatctccga aagtccccag agtactgtca ctatctcgag gcagttaagg
     7501 acggggagct tacgctgccg gatttcaaga tagtaagccg acccgactag ctttaagcct
     7561 agttactgac tagtccacag gacgacaatg gcgtgcctga tatccatcca tatgaagtct
     7621 actgccgagt gaagggatgc ctcaagcgta cagtgagtct actgctatac tagtttctga
     7681 ctagttatta actagttgac aggttccctc tgccaacaga aacatattgg tcaagcactt
     7741 gaaggacaag aactcccacg gcatggagtt tacattgcac aatggacctc ccactatgaa
     7801 ggaactgatg gaggccaaag gcaagtccta tctagattac ttggtgacta gtccctgact
     7861 agtcaagtag catggtatga aggcttgttt gaaggcactg ttctcccaac cccgactcct
     7921 accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc taactagtgt attagctaat
     7981 atatagcagc accaagtcca aggaccacaa tactaagggt gtcgagaatt cgtgagtttc
     8041 ttctcccatt tcaactagtc cttgactagt cactaactac tttgcagcaa cgagggtgaa
     8101 gctggaaatg atcaggacaa tggcgagggc ccgtaagtac agccattcaa tgcagactag
     8161 ttgctaacta atctgtgact agttcaagtg gtccgtacgc cgtgcatacc cctgtgactg
     8221 gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa aggaaaggca agttacattc
     8281 agccccgtac ctaggatcag tgctaattta taacctctag ccactcttta tgcagatccg
     8341 ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa ggaaccatac cctgcaagac
     8401 ctgtcgcaac gcaaagggca aaggtaagct atccaagcta gtttgggact agattctaac
     8461 tagtctcagc accgtgtggt tcaaagccat attgcgagtt ttggcgcttt ttctcatcga
     8521 ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc agactcagac aaagaccaac
     8581 tagttattga ccagtcttct agaaggctct gttgtggatc ttgaggccct ggagagttcc
     8641 tccaacaatc cggagacaag caagtcctcg tcggactagt cactaactag actctaacta
     8701 gttgcagaca tggataatgc aaaagagaca agcaatgaag aaagtggtaa gacatttctc
     8761 ctttgtggtt ctggactagt ctttgactag tcacagtctt aaacaaggaa aatgagcatg
     8821 aaaatgagga ggaaaaggct gctgagcccg aggaagtgca gggtgatggc agacatggta
     8881 ggttaatacc ttgttagtta ttgctagtca ctgactagtc aataactagt ctctgaacac
     8941 cttgcaatca ctccgtttgc gcagctgaac agtggtgagg ataatagtag taagttattc
     9001 tagcttcaga gttataggag actagatact aactagtatt agttgcaact aacctggatc
     9061 tcagagactt tggcctcaat ctagaatcta tctagttgtc aactagactg tggtatcatt
     9121 gtcttttatt ttcctagtcc tggaactagc ttctaactag tctccctaat atgtggctgt
     9181 cttgtttttt ttttttgttt ccctacccgg atatctagtc cccttctagg ttctgttaac
     9241 ctctcgggct ctgatttagt ttaacgcaaa cctgagatta gtttctaact agtctctagg
     9301 ttttctatcc acctttaatt gtaataataa atacaagcaa cgtttatacg tcaaaagcat
     9361 ttataaactt ttaccctaaa gtagcttgct tgtgtgttta gtttataatt agtctcttat
     9421 taatttgatg taggtaagcc cgccacaaat atatattttt aacaagatac cgtggaaaaa
     9481 cttcgtgcta tcacaaaaca gtatacaaaa aataagctgg ctttccccgt caagctctaa
     9541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     9601 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     9661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9721 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     9781 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaatg gggtgacgat
     9841 gagccgctct tgcatctttg tttgtattat actgtctttc ttgttacaca taattattct
     9901 agaatgcccc accgttacat acgggacaca gccatttaca tatgcatgtg gattacgagc
     9961 taacgagttc attcaaatct cagaactatc acataatcat cattccccta tcgtcaaaga
    10021 ccgtaagaca aatccggttc atgcactgaa cccattcggg tagtgagtca tttactcagc
    10081 acactcgcgc tgacgctcgt cgaacacctt caatgcctcc tcggcagcct tgacaccact
    10141 gagaaccatg gcaccgaagg tagggcccat gcggttaaag ccatcaattt cagacagctc
    10201 cataccgccg attatcaagc ccttagtaac ctcgcgggtg ttcttgacga tggcatcctg
    10261 cggccgccca gaaaattacg ggtatgtcct ccactgtgtt gaagtcagaa aaacaatcga
    10321 taatttgaag ctcattaaaa tgccagttcc gtcgtcgccg ttgccctagg agctattcca
    10381 gtgcttagct tcattcatgg atgtatcgtg actggccttc ggaaggatgc caaggtcccc
    10441 tatccgcaca gctatgcgac cgttgagcaa tgcaagtcaa atgtgtgcat acctggtctt
    10501 tggtttggta caccgctatg tgaagattat taatgctgaa gtcatgatac aggccaaagc
    10561 cgagcaattt aactgcgcgc agcgggccca cgctaactac ctcgagaata tgcctcaaac
    10621 aatgctcttc accctcttcg cgggtctgaa ataccctcac ctggcaacag caattggcgc
    10681 ttcatggctg tttttccgat ctctctactt gtacggctat gtgtactcgg gtaagccaca
    10741 aggcaagggc agattgctgg gaggtttctt ctggttttct caaggcgctc tgtgggctct
    10801 gagtgtgttt ggtgttgcca aagacatgat ctcttactga gagttattct gtgtctgacg
    10861 aaatatgttg tgtatatata tatatgtacg ttaaaagttc cgtggagtta ccagtgattg
    10921 accaatgttt tatcttctac agttctgcct gtctacccca ttctagctgt acctgactac
    10981 agagtagttt aattgtggtt gaccccacag tcggaggcgg aggaatacag caccgatgtg
    11041 gcctgtctcc atccagattg gcacgcaatt tttacacgcg gaaaagatcg agatagagta
    11101 cgactttaaa tttagtcccc ggcggcttct attttagaat atttgagatt tgattctcaa
    11161 gcaattgatt tggttgggtc accctcaatt ggataatata cctcattgct cggctacttc
    11221 aactcatcaa tcaccgtcat accccgcata taaccctcca ttcccacgat gcagattttc
    11281 gtcaagactt tgaccggtaa aaccataaca ttggaagttg aatcttccga taccatcgac
    11341 aacgttaagt cgaaaattca agacaaggaa ggtatccctc cagatcaaca aagattgatc
    11401 tttgccggta agcagctaga agacggtaga acgctgtctg attacaacat tcagaaggag
    11461 tccaccttac atcttgtgct aaggctaaga ggtggttatc acggatccgg agcttggctg
    11521 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgtc gtccaagtcg
    11581 caattgactt acggtgctcg agccagcaag caccccaatc ctctggcaaa gagacttttt
    11641 gagattgccg aagcaaagaa gacaaacgtt accgtctctg ctgatgtgac gacaacccga
    11701 gaactcctgg acctcgctga ccgtacggaa gctgttggat ccaatacata tgccgtctag
    11761 caatggacta atcaactttt gatgatacag gtctcggtcc ctacatcgcc gtcatcaaga
    11821 cacacatcga catcctcacc gatttcagcg tcgacactat caatggcctg aatgtgctgg
    11881 ctcaaaagca caactttttg atcttcgagg accgcaaatt catcgacatc ggcaataccg
    11941 tccagaagca ataccacggc ggtgctctga ggatctccga atgggcccac attatcaact
    12001 gcagcgttct ccctggcgag ggcatcgtcg aggctctggc ccagaccgca tctgcgcaag
    12061 acttccccta tggtcctgag agaggactgt tggtcctggc agagatgacc tccaaaggat
    12121 cgctggctac gggcgagtat accaaggcat cggttgacta cgctcgcaaa tacaagaact
    12181 tcgttatggg tttcgtgtcg acgcgggccc tgacggaagt gcagtcggat gtgtcttcag
    12241 cctcggagga tgaagatttc gtggtcttca cgacgggtgt gaacctctct tccaaaggag
    12301 ataagcttgg acagcaatac cagactcctg catcggctat tggacgcggt gccgacttta
    12361 tcatcgccgg tcgaggcatc tacgctgctc ccgacccggt tgaagctgca cagcggtacc
    12421 agaaagaagg ctgggaagct tatatggcca gagtatgcgg caagtcatga tttcctcttg
    12481 gagcaaaagt gtagtgccag tacgagtgtt gtggaggaag gctgcataca ttgtgcctgt
    12541 cattaaacga tgagctcgtc cgtattggcc cctgtaatgc catgttttcc gcccccaatc
    12601 gtcaaggttt tccctttgtt agattcctac cagtcatcta gcaagtgagg taagctttgc
    12661 cagaaacgcc aaggctttat ctatgtagtc gataagcaaa gtggactgat agcttaatat
    12721 ggaaggtccc tcaggacaag tcgacctgtg cagaagagat aacagcttgg catcacgcat
    12781 cagtgcctcc tctcagacag aataagtcgc cataagttat cgaccgaacc taggtagggt
    12841 atatttggtg acgacaatac ctcccgacga tacctggatt agacaccgta tgccgcgaat
    12901 taacgccttc cgaccaagtc ccttttggtg atcgacactt taggatcatc tcctgccagt
    12961 cgcccagact gacggtgtta tggtggcgcg ctatggcttt cctagccggt caagctccgt
    13021 gcactagaga gcggagaagg agggggagtt gatagggaat gatatctagg tagagaagta
    13081 ctccaaccag agatatgcgg atcaatgctc cggcagttgt tagcataccc caacctcgtt
    13141 taagtatcct cttcttccat ccatctttat cattatttgt ctatgtgcat ccctagatca
    13201 cgtcaattat ttcgataaat ggatacgtgc actcgggaat gcgtgattgg ctcgtctcgt
    13261 gttctcagtt cctcattccc ccccacgtta cattgacaac ggagtattag cctatggtcc
    13321 tgttcgataa gcttgctcca tcgcctccac tacgtcaagc ggagtatcag acatgagctc
    13381 taccccagat atcgcctgct tatcgctggt tgctttgtca tgcaagtaca gatcacatcg
    13441 gcaattctga taacagatgg tgccattgtc ccgtaatcaa ttgcccattg aaaaaggaag
    13501 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    13561 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    13621 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    13681 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    13741 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    13801 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    13861 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    13921 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    13981 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    14041 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    14101 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    14161 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    14221 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    14281 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    14341 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    14401 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    14461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    14521 atcaaaggat cttc
//
