LOCUS       sequence-49595-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     NHEJ
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDR125
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(148..165)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(166..177)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcataacagt tacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
       61 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
      121 agtacttctc gacaagcttg gtacctagtg atggtgatgg tgatggcgtc cctcgatctc
      181 gagaatcaaa tactggtttt cttcttgtaa ttcacacttg tctattgaat cagttaccca
      241 actttctttt aggattttaa actttctctt aaaagttctt ctaaaagctt taaaatctgc
      301 aacacgacta tgatcttccc caattattac atgagacact ccctcagcta aacaagaaac
      361 tacttttgct ccatgaaacc gaagctccaa ggctttaata gctaaccttg tcccctcatt
      421 tttggtactc aggtcattaa taacagcata cgagtccaaa taaacggtgt ggcgtcgaaa
      481 catactgaga ggagagcaat cccaggaata ccgatattct aaatcagcaa tcagagaagc
      541 catttcttca ggagtctgct cgttagaatt tttaattcct gagaatactt ccttcagttg
      601 gttcaagtct gtatcaatga aataactatc accatagcaa tcatattcac gggcaaaatg
      661 ttctttggtt gatgggcaca tatgaatcat aaagcgaggc tgccatggta caaagctttt
      721 ggtcttaaaa cattctaaaa gccatgcagg cttgacaaca tcatgtttat ttgacaaaat
      781 tatgtttttc actctgatgt tctcagaccc tgcaattaca cagtacgtgt ctgggcctgg
      841 attttgtact atataaccac caaattctgc aattctggtt ctccaggtca ggctttggct
      901 ggctatctgt tccact
//
