LOCUS       sequence-495965-       10092 bp    DNA     circular SYN 23-DEC-2025
DEFINITION  tet inducible bidirectional plasmid expressing GFP in one direction 
            and in the other a human DMPK genomic segment containing exons 11-15
            with 960 interrupted CTG repeats in exon 15 located.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Lee JE, Bennett CF, Cooper TA
  TITLE     RNase H-mediated degradation of toxic RNA in myotonic dystrophy type
            1.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4221-6. doi: 
            10.1073/pnas.1117019109. Epub 2012 Feb 27.
  PUBMED    22371589
REFERENCE   2  (bases 1 to 10092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1117019109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-03-13- 13"; volume: "109"; issue: "11"; pages: "4221-6"
COMMENT     Sequence Label: pBItetDT960GFP
FEATURES             Location/Qualifiers
     source          1..10092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(56..74,75..97,98..116,117..139,140..158,159..181,
                     182..200,201..223,224..242,243..265,266..284,285..307,
                     308..326)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        359..397
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      541..550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(547..549,550..552,553..1266)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(547..549,550..552,553..1263)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(592..613)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(853..872)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1200..1221
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          1352..1924
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2056..2075)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2121..2176
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2175..2194)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2574..2591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2745..3333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2825..2844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3504..4295,4296..4364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4127..4146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4365..4469)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4537..4555
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aggcgatctg acggttcact aaacgagctc tgcttatata ggtcgagttt accactccct
       61 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
      121 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
      181 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      241 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      301 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg
      361 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
      421 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
      481 ccgcggcccc gaattcgagc tcggtacccg gggatcctct agtcagctga cgcgccggtc
      541 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      601 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      661 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      721 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      781 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      841 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      901 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      961 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1021 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1081 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1141 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1201 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1261 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     1321 ttgctttatc tctagagctg agaacttcag ggtgagtttg gggacccttg attgttcttt
     1381 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
     1441 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
     1501 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
     1561 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
     1621 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
     1681 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
     1741 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt
     1801 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
     1861 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct
     1921 acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcac
     1981 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     2041 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     2101 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     2161 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     2221 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     2281 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     2341 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     2401 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     2461 actactccca gtcatagctg tccctcttct cttatggaga tccctcgact gcattaatga
     2521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3541 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3601 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3661 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3721 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3781 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3841 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3901 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3961 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4021 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4081 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     4141 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4201 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4261 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4321 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4381 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4441 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4501 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     4561 cgtcttcact cgacgcggcc agcttggtcg agctggatac ttcccgtccg ccagggggac
     4621 atgccggcga tgctgaaggt cgcgcgcatt cccgatgaag aggccggtta ctcgagcgca
     4681 ggcgcagtgg aaggaggatg gccgcgcgcg ctgccagccc agccccctct tctcgacgct
     4741 cggtggcaca gctgggccac agctgggcgg gggcggtgcc tccgggtggc ccgctcgccc
     4801 tcctattggc cggacgccaa agccccgccc cgtggctttt cctcccccaa ccctgattcg
     4861 gccgcttcgc atcccgctag ctcctcccag accttcggcc gcctccacac gcctccggat
     4921 tggcccgctg cggagcctcc ggcccacaac gcaaaccgcg gacactgtgg agtccagagc
     4981 tttgggcaga tggagggcct tttattcgcg agggtcgggg gtgggggtcc taggtgggga
     5041 cagacaatag ataccgagga atgtcggggt ctcagtgcat ccaaaacgtg gattggggtt
     5101 gttgggggtc ctgtagcctg tcagcgagtc ggaggacgag gtcaataaat atccaaaccg
     5161 ccgaagcggg cggggccggc tggggctccg agagcagcgc aagtgaggag gggggcgcgg
     5221 gatccccgaa aaagcgggtt tggcaaaagc aaatttcccg agtaagcagg cagagatcgc
     5281 gccagacgct ccccagagca gggcgtcatg cacaagaaag ctttgcactt tgcgaaccaa
     5341 cgataggtgg gggtgcgtgg aggatggaac acggacggcc cggcttgctg ccttcccagg
     5401 cctgcagttt gcccatccac gtcagggcct cagcctggcc gaaagaaaga aatggtctgt
     5461 gatcccccgt cgagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     5521 agcagcagca gcagtcgagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     5581 gcagcagcag cagcagcagt cgagcagcag cagcagcagc agcagcagca gcagcagcag
     5641 cagcagcagc agcagcagca gcagtcgagc agcagcagca gcagcagcag cagcagcagc
     5701 agcagcagca gcagcagcag cagcagcagt cgagcagcag cagcagcagc agcagcagca
     5761 gcagcagcag cagcagcagc agcagcagca gcagtcgagc agcagcagca gcagcagcag
     5821 cagcagcagc agcagcagca gcagcagcag tcgagcagca gcagcagcag cagcagcagc
     5881 agcagcagca gcagcagcag cagcagcagc agcagtcgag cagcagcagc agcagcagca
     5941 gcagcagcag cagcagcagc agcagcagca gcagcagcag tcgagcagca gcagcagcag
     6001 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagtcgag cagcagcagc
     6061 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag tcgagcagca
     6121 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagtcgag
     6181 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
     6241 tcgagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     6301 agcagtcgag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     6361 gcagcagcag tcgagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
     6421 cagcagtcga gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     6481 agcagcagca gtcgagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     6541 gcagcagcag cagcagtcga gcagcagcag cagcagcagc agcagcagca gcagcagcag
     6601 cagcagcagc agcagcagca gtcgagcagc agcagcagca gcagcagcag cagcagcagc
     6661 agcagcagca gcagcagcag cagcagtcga gcagcagcag cagcagcagc agcagcagca
     6721 gcagcagcag cagcagcagc agcagcagca gtcgagcagc agcagcagca gcagcagcag
     6781 cagcagcagc agcagcagca gcagcagcag cagcagtcga gcagcagcag cagcagcagc
     6841 agcagcagca gcagcagcag cagcagcagc agcagcagca gtcgagcagc agcagcagca
     6901 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagtcgagca gcagcagcag
     6961 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagtc gagcagcagc
     7021 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagtcgagca
     7081 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     7141 gcagcagtcg agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
     7201 cagtcgagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     7261 agcagcagtc gagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     7321 gcagcagcag cagtcgagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
     7381 cagcagcagc agcagcagtc gagcagcagc agcagcagca gcagcagcag cagcagcagc
     7441 agcagcagca gcagcagcag cagtcgagca gcagcagcag cagcagcagc agcagcagca
     7501 gcagcagcag cagcagcagt cgagcagcag cagcagcagc agcagcagca gcagcagcag
     7561 cagcagcagc agcagcagca gcagtcgagc agcagcagca gcagcagcag cagcagcagc
     7621 agcagcagca gcagcagcag cagcagcagt cgagcagcag cagcagcagc agcagcagca
     7681 gcagcagcag cagcagcagc agcagcagca gcagtcgagc agcagcagca gcagcagcag
     7741 cagcagcagc agcagcagca gcagcagcag cagcagcagt cgagcagcag cagcagcagc
     7801 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagtcgagc agcagcagca
     7861 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagt cgagcagcag
     7921 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagtcgag cagcagcagc
     7981 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag tcgagcagca
     8041 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagtcgag
     8101 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagtcgagc
     8161 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagt
     8221 cgagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     8281 gcagcagtcg agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
     8341 cagcagcagc agtcgacatt cccggctaca aggacccttc gagccccgtt cgccggccgc
     8401 ggacccggcc cctccctccc cggccgctag ggggcgggcc cggatcacag gactggagct
     8461 gggcggagac ccacgctcgg agcggttgtg aactggcagg cggtgggcgc ggcttctgtg
     8521 ccgtgccccg ggcactcagt cttccaacgg ggccccggag tcgaagacag ttctagggtt
     8581 cagggagcgc gggcggctcc tgggcggcgc cagactgcgg tgagttggcc ggcgtgggcc
     8641 accaacccaa tgcagcccag ggcggcggca cgagacagaa caacggcgaa caggagcagg
     8701 gaaagcgcct ccgataggcc aggcctaggg acctgcgggg agagggcgag gtcaacaccc
     8761 ggcatgggcc tctgattggc tcctgggact cgccccgcct acgcccatag gtgggcccgc
     8821 actcttccct gcgccccgcc cccgccccaa cagcctacag ctgttgttag tccactcgca
     8881 cgcctcgaat cccgtccgaa ctcgtcattg gctgcttcct agcggcctgt gttgattggc
     8941 tgcccgaaga tccgccctcc tgccgtgggc ccagccccgc aaatgcgcag ctaagcgggt
     9001 ggcacggggc gggtggagcg cggggcgcga cggcggaggg gggcgtgggc agccggacgt
     9061 accctggcag ggagcagcag gtggcggcgg tgcatggggc ctggccccac cagcgggcac
     9121 tggcccacag ccacggccgg ggggccatct agctggagag agaagggaca ggtgacccga
     9181 tcggagccca gcccagccct cagcggtggg gcgagagaca gcgaggggaa tcgaggttgg
     9241 ggaggttatc tagggagatc ccggagggaa tctggtgagg cctgaacgga gggagatctg
     9301 gggctgaata aagggcttct gccctctaaa gtcgcaaaga cgtagggtga gccctatatc
     9361 tggacgggga gaccaggagc cagggagggg atctgcagaa tgggcagcag gtctgaggca
     9421 ggggaaagag aggggtctta catgggaagg tggatccgtg gcccggggac tggggacccc
     9481 cgtgacagct ggaaggagaa gaaagaggca tagggcgcgt ggaggggcga aggagggcgg
     9541 tggcgcggcg tgccccagcg tgggtccctt ccctcctcca ggtgtctata cacgccccgc
     9601 ggagcagacg gcccacctcc tcccggtcct ccggggaagg ggacacatga gggactcacc
     9661 tgtggctccc tctgcctgca gcaactccat ccgctcctgc aactgccgga cgtgtgcctc
     9721 taggtcccgg ttccgagcct ctgcctcgcg tagttgactg tggggaggta aggacggtga
     9781 gtccgtccgg gccggacgag aggggctgcc aagggttgcc accggcccgc atcccggccc
     9841 cggccccggc cccgatcccg acctggcgaa gttctggttg tccgtgcgga tggcctccat
     9901 ctcccggctc aggctctgcc gggtgagcac ctcctcctcc agggcttcct ggagctcccg
     9961 cagcgtcacc tcggcctcag cctgatctga gtccggtagc tcgacgcggc cgctgcagga
    10021 attcggggcc gcggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg
    10081 gatggcgtct cc
//
